We narrowed to 7,460 results for: Lif

1. pLV-mCherry-FKBP-TIAM1(DHPH)

   Plasmid

   #176138

   Purpose

   Heterodimerization with FRB (rapamycin), Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5

   Plasmid

   #125234

   Purpose

   Expresses SPARK oβ2AR transmembrane component in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5
   

   Use

   AAV

   Tags

   Flag, V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-mCherry-mCherry-FLARE-Cameleon

   Plasmid

   #123352

   Purpose

   Red-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   mCherry-mCherry-FLARE-Cameleon
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CAG-DIO-erHRP(N175S mutant)

   Plasmid

   #79906

   Purpose

   Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

   Depositor

   Joshua Sanes

   Article

   Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

   Insert

   erHRP(N175S mutant)
   

   Use

   AAV and Cre/Lox

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   N175S mutation

   Promoter

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ncam1_1.18.1-AP-His

   Plasmid

   #71962

   Purpose

   Expresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ncam1_1.18.1 (Ncam1 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ncam1_0.0.0-AP-His

   Plasmid

   #71960

   Purpose

   Expresses the extracellular region of the NCAM1, isoform 0.0.0 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ncam1_0.0.0 (Ncam1 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sema3a(L, del13)-AP-His

   Plasmid

   #72011

   Purpose

   Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 13), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3a (Sema3a Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV HA hRB delta CDK + S780

   Plasmid

   #58915

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S780 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pLKO.1-shRBFOX1-3261

   Plasmid

   #115458

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   shRBFOX1-3261 (RBFOX1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-shRBFOX1-3260

    Plasmid

    #115457

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shRBFOX1-3260 (RBFOX1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-22HT-MSI2(8-193)

    Plasmid

    #60356

    Purpose

    Express the RBD of human MSI2 in bacterial cells for protein purification

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    human MSI2 (8-193) (MSI2 Human)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    contains amino acids 8-193 of MSI2

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

    Plasmid

    #123346

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-CKAR
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-mCherry-SPASTIN, F124D

    Plasmid

    #180648

    Purpose

    Lentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Starts at M87, F124D mutation, has siRNA resistan…

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL007_PB_pCMV-H2B-mIFP-T2A-rTetR-ratKRAB-zeo

    Plasmid

    #179438

    Purpose

    Expresses rTetR-KRAB(rat) for gene silencing, integration by Piggybac

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    H2B-mIFP-T2A-rTetR-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Sema7a-AP-His

    Plasmid

    #72049

    Purpose

    Expresses the extracellular region of the Sema7A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema7a (Sema7a Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Plxna1-AP-His

    Plasmid

    #71996

    Purpose

    Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna1 (Plxna1 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC

    Plasmid

    #114376

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP, Kv2.1C, and TlcnC

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV HA hRB delta CDK + S811

    Plasmid

    #58919

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S811 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S811 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-GFP-GFP-FLARE-AKAR

    Plasmid

    #123332

    Purpose

    Green-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    GFP-GFP-FLARE-AKAR
    

    Use

    Tags

    6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac_His-ZZ-TEV-Lis1-SNAPf

    Plasmid

    #133073

    Purpose

    Plasmid for insect cell expression of human full-length LIS1 with an N terminal His-ZZ-LTLT tag and C terminal SNAPf tag

    Depositor

    Simon Bullock, Andrew Carter

    Article

    Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

    Insert

    Lis1 (PAFAH1B1 Human)
    

    Use

    Tags

    8x His, Linker-TEV-linker_TEV, SNAPf, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV HA hRB delta CDK + S807

    Plasmid

    #58918

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S807 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S807 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pCMV HA hRB delta CDK + S788

    Plasmid

    #58916

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S788 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S788 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pCMV HA hRB delta CDK + T356

    Plasmid

    #58911

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T356 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T356 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pCMV HA hRB delta CDK + T821

    Plasmid

    #58920

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T821 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T821 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pKrox24(MapErk)DsRed

    Plasmid

    #200114

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MapErk sequence in minimal promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    5 copies of designed MapErk sequence, no other pr…

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MLS-mir-17-92-Mut92

    Plasmid

    #64094

    Purpose

    expresses a mutated version of mir-17-92 (mutation of miR-92 seed)

    Depositor

    Lin He

    Article

    Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

    Insert

    miR-17-92 (MIR17HG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutation of miR-92 seed sequence

    Promoter

    MSCV

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pCMV HA hRB delta CDK + T826

    Plasmid

    #58921

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T826 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T826 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pLNCX2-mCherry-CHMP2A-siRNAres

    Plasmid

    #115332

    Purpose

    expresses siRNA-resistant mCherry-CHMP2A in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mutated to be resistant to siRNA knockdown

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Sema4c-AP-His

    Plasmid

    #72030

    Purpose

    Expresses the extracellular region of the Sema4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4c (Sema4c Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Dscam1_7.27.30 EC10-Fc-His

    Plasmid

    #72190

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.30
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge