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Showing: 4201 - 4250 of 13557 results

  3. pKS7107

    Plasmid
    #89051
    Purpose
    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9
    Depositor
    Ervin Welker
    Article
    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.
    Inserts
    Nm crRNA
    Nm tracrRNA
    hNmCas9
    Use
    CRISPR
    Tags
    HA tag, NLS, and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a and human U6
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    89051-plasmid-map-sequence-id-176334

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  7. pUC19-G2ZYP2

    Plasmid
    #103110
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103110-plasmid-map-sequence-id-201819

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  12. pET28a-HsSENP6

    Plasmid
    #71469
    Purpose
    Encodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 6 (SENP6 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pETDuet-1-6xHis-TEV-Nsp8

    Plasmid
    #201022
    Purpose
    Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.
    Depositor
    Sharon Rozovsky
    Article
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Insert
    non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201022-plasmid-map-sequence-id-394460

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  17. pUC19-Q927P4

    Plasmid
    #103131
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103131-plasmid-map-sequence-id-201895

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  23. pCMV dCas9-MC (eGFP)

    Plasmid
    #89931
    Purpose
    Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.
    Depositor
    Carl Novina
    Article
    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.
    Insert
    dCas9-MC
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deactivated Cas9, fragment of M.SssI (residues 27…
    Promoter
    CMV
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89931-plasmid-map-sequence-id-177995

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  29. pAAV-hSyn-sbGLuc-CheRiff-EGFP

    Plasmid
    #114108
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.
    Insert
    Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    114108-plasmid-map-sequence-id-252563

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  30. pMuLE ENTR SV40-hCas9 L3-L2

    Plasmid
    #62133
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    human codon optimized Cas9
    Use
    CRISPR; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62133-plasmid-map-sequence-id-101417

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  50. pET-dHeFSpCas9-VP64-6xHis

    Plasmid
    #92119
    Purpose
    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, NLS, and VP64
    Expression
    Bacterial
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    T7
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92119-plasmid-map-sequence-id-194893

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Showing: 4201 - 4250 of 13557 results