We narrowed to 14,145 results for: TIM
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Plasmid#71345PurposeExpress Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian celDepositorInserthOTC_Cyan Nano-lantern(Ca2+)
ExpressionMammalianAvailable SinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-DRE-1-pcDNA3.1-
Plasmid#52517Purposeexpresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminusDepositorAvailable SinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPD95.75-cYAP-1
Plasmid#66857PurposeGenerate transgenic C.elegans with cYAP-1 fused to GFPDepositorInsertC.elegans YAP-1 (yap-1 Nematode)
TagsGFPExpressionWormMutationSerine104 is mutated to alaninePromoterC. elegans 5'UTRAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPD95.75-cYAP-1 S104A
Plasmid#66858PurposeGenerate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanineDepositorAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NES-CAGEc-AF9c (Npu)
Plasmid#241413PurposeProximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.DepositorInsertNES-CAGEc-AF9c (Npu) (MLLT3 Human, Synthetic)
Tags3xFLAG and HAExpressionMammalianPromoterCMVAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NES-CAGEc-ETOc (Npu)
Plasmid#241409PurposeProximity-triggered Protein Trans-Splicing to generate the splice product AML1-ETO, Extended Figure 3 in Nature Chemical Biology 20.10 (2024): 1353-1360.DepositorInsertNES-CAGEc-ETOc (Npu) (RUNX1T1 Human, Synthetic)
Tags3xFLAG and HAExpressionMammalianPromoterCMVAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NES-CAGEc-AF9c (Nrdj1)
Plasmid#241414PurposeProximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Figure 2 & Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.DepositorInsertNES-CAGEc-AF9c (Nrdj1) (MLLT3 Human, Synthetic)
Tags3xFLAG and HAExpressionMammalianPromoterCMVAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIDTsmart-RC2-delVP12-VP12-454TAG
Plasmid#240061PurposeExpress AAV2 capsid with ncAA mutant at site 454 of VP1 and VP2DepositorInsertsRC2-DelVP12
VP1-454-TAG
VP2-454-TAG
UseAAVExpressionMammalianAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIDTsmart-RC2-KO-delVP1-VP1-454TAG
Plasmid#240062PurposeExpress AAV2-KO (R585A,R588A) capsid with ncAA mutant at site 454 of VP1DepositorInsertsRC2-DelVP1-R585,588A
VP1-454-TAG-R585,588A
UseAAVExpressionMammalianAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(B)
Plasmid#236041PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
UseCRISPRPromoterquorum sensing promoterAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(A)
Plasmid#236039PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
UseCRISPRPromoterquorum sensing promoterAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIN3 (gpdA-cTerm-Citrine2-ptrA)
Plasmid#188737PurposeExpresses Citrine2 for C-terminal taggingDepositorInsertgdpA-Citrine2 PtrA
UseFilamentous fungal expressionTagsCitrine2Available SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIN4 (gpdA-nTerm-Citrine2-ptrA)
Plasmid#188738PurposeExpresses Citrine2 for N-terminal taggingDepositorInsertgdpA-Citrine2 PtrA
UseFilamentous fungal expressionTagsCitrine2Available SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIN5 (gpdA-cTerm-mTurqoise2-ptrA)
Plasmid#188739PurposeExpresses mTurqoise2 for C-terminal taggingDepositorInsertgdpA-mTurquoise PtrA
UseFilamentous fungal expressionTagsmTurquoiseAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIN6 (gpdA-nTerm-mTurqoise2-ptrA)
Plasmid#188740PurposeExpresses mTurqoise2 for N-terminal taggingDepositorInsertgdpA-mTurqoise2 PtrA
UseFilamentous fungal expressionTagsmGreenlanternAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUt-MONARCH 1.0_crEGFP
Plasmid#224790PurposeLPUtopia matching RMCE donor plasmid with MONARCH 1.0 circuit with crRNA targeting EGFP. Use BlastR for positive and HSV-TK for negative selectionDepositorInsertcrEGFP
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterCMV-d2iAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUt-MONARCH 2.0_crEGFP
Plasmid#224793PurposeLPUtopia matching RMCE donor plasmid with MONARCH 2.0 circuit with crRNA targeting EGFP.DepositorInsertscrEGFP
Triplex stablier
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterCMV-d2iAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-2O-crBFP_EF1a-BFP
Plasmid#224783PurposeBFP-targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and target BFP protein expressed from EF1a promoterDepositorInsertcrBFP
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhU6-2xTetOAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-2O-crEGFP_EF1a-BFP
Plasmid#224784PurposeEGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoterDepositorInsertcrEGFP
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhU6-2xTetOAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-crBFP_EF1a-BFP
Plasmid#224785PurposeBFP targeting crRNA for RfxCas13d expressed from hU6 promoter and target BFP protein expressed from EF1a promoterDepositorInsertcrBFP
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhU6 and hU6-2xTetOAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-CrEGFP_EF1a-BFP
Plasmid#224786PurposeEGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoterDepositorInsertcrBFP
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhU6 and hU6-2xTetOAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIV-Streptomyces
Plasmid#226250PurposeExpresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIV-Streptomyces)
TagsHis6ExpressionBacterialAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B2-ELP-HIS-ybbR
Plasmid#169134PurposeE. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgß-FIVAR-ELP-HIS-ybbR
Plasmid#169136PurposeE. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertFIVAR (found in various architectures)
Tags3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…ExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-VHH-ddFLN4-HIS-ybbR
Plasmid#169137PurposeE. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertVHH
Tags6x His tag, ddFLN4 tag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B1-ELP-HIS-ybbR
Plasmid#169133PurposeE. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperior.retro.neo GFP shDNM1L
Plasmid#220356PurposeRetroviral expression vector for an shDNM1LDepositorAvailable SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-56
Plasmid#225073PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-56)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2C
Plasmid#225070PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2C)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2A
Plasmid#225068PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2A)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2B
Plasmid#225069PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2B)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-57
Plasmid#225074PurposeExpresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coliDepositorInsertLanA(A) (LanII-57)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII-6
Plasmid#225076PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coliDepositorInsertLanA(A) (LanIII-6)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2E
Plasmid#225072PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2E)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-23
Plasmid#225067PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-23)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2D
Plasmid#225071PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2D)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-PD-L2-His10
Plasmid#223578PurposeProtein purification of human PD-L2 with SNAP and 10xHis tags in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4316
Plasmid#200171PurposePflp-18 LoxP EBFP (stop) LoxP twk-40(gf) GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40(gf) GFPDepositorAvailable SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH4018
Plasmid#200043PurposePrig-3 FRT let858 (stop) FRT zif-1 SL2 EBFP unc-54 3' UTR C.elegans AVA and other neurons expression of zif EBFPDepositorAvailable SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro
Plasmid#189634PurposeLentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs librariesDepositorInserthU6 Cas9-Cas12a gRNA cassette, PGK puro cassette
UseCRISPR and LentiviralExpressionMammalianAvailable SinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF366
Plasmid#155332PurposeExpression of FLuc under the control of AtAP1 promoterDepositorInsertPAtAP1-FLuc-TSV40
UseLuciferase and Synthetic BiologyExpressionPlantAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pShuttle.Cre
Plasmid#192930PurposeGateway compatible vector with BsaI cloning site and Cre expressionDepositorTypeEmpty backboneUseGateway-compatible cloning vectorPromoterCMVAvailable SinceDec. 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pRB116.2
Plasmid#181946PurposeaTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129DepositorInsertTagsmNeonGreenExpressionBacterialPromoterTorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106Available SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB120.2
Plasmid#181947PurposeaTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129DepositorInsertTagsmNeonGreenExpressionBacterialPromotermNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106Available SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB133.2
Plasmid#181950PurposeaTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirKDepositorInsertPhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)
TagsmNeonGreenExpressionBacterialPromoterPhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106Available SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0-EGFP-HRasC20
Plasmid#187252PurposeLenticiral expression of EGFP-HRasC20DepositorAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMERVK9c_1 (inducible)
Plasmid#189957PurposeshRNA mediated knockdownDepositorInsertERV_MMERVK9c
UseLentiviralAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMERVK10c_2 (inducible)
Plasmid#189956PurposeshRNA mediated knockdownDepositorInsertERV_MMERVK10c
UseLentiviralAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMETn_2 (inducible)
Plasmid#189954PurposeshRNA mediated knockdownDepositorInsertERV_MMETn
UseLentiviralAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only