We narrowed to 2,496 results for: 1186

1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pDD317 (Pmyo-2::GFP + SEC)

   Plasmid

   #73344

   Purpose

   SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

   Insert

   Pmyo-2::GFP + SEC
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Promoter

   myo-2

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMPE-ePPEplus

   Plasmid

   #205242

   Purpose

   For multiplex plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PxylA

   Plasmid

   #55178

   Purpose

   Xylose-inducible promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   xylA promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Promoter

   xylA

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQTEV- PRDX2

   Plasmid

   #31338

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   PRDX2 (PRDX2 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDT-sgRNA-XMAS-1x

   Plasmid

   #164413

   Purpose

   Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOCC167

   Plasmid

   #118909

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNTI661 pRPR1(TetO)-sgRNA

   Plasmid

   #139475

   Purpose

   Vector for tet-regulated sgRNA expression in yeast

   Depositor

   Nicholas Ingolia

   Article

   McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

   Insert

   single guide RNA scaffold
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   pRPR1(TetO)

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQTEV-PTD004

   Plasmid

   #34798

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   GTP-binding protein PTD004 (OLA1 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-neo-Strep_Flag_Cterm

   Plasmid

   #102645

   Purpose

   Epitope tag c-terminus with SBP-Flag moiety

   Depositor

   Michael E. Wright

   Article

   Hsiao et al BMC Cancer. 2015 Mar 31;15:204. doi: 10.

   Type

   Empty backbone

   Tags

   SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

   Expression

   Mammalian

   Available Since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS1ClacZ

    Plasmid

    #55171

    Purpose

    lacZ-reporter vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

11. BS-TRP1-TetOff(7repeats)-GFP-Pho8

    Plasmid

    #207011

    Purpose

    Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a(+)::MBP-NL3F10H

    Plasmid

    #141291

    Purpose

    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    MBP-NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3), His (x10), and MBP

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PTEF1-yEGFPCLN2PEST-pRS406

    Plasmid

    #64406

    Purpose

    TEF promoter driven GFP expression

    Depositor

    Claudia Vickers

    Article

    Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

    Insert

    TEF1 promoter (TEF1 Budding Yeast)
    

    Tags

    yEGFPCLN2PEST (destabilized yeast EGFP)

    Expression

    Yeast

    Promoter

    TEF1 promoter

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pILGFPI7

    Plasmid

    #83555

    Purpose

    Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    CUP1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    CUP1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDECKO_GFP

    Plasmid

    #72619

    Purpose

    Expresses two gRNAs targeting GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward GFP
    

    Expression

    Mammalian

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pILGFPB5A

    Plasmid

    #83556

    Purpose

    Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    GAL1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pILGFP91

    Plasmid

    #83548

    Purpose

    Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    PGK1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    PGK1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGM4723:TpCC_Urease

    Plasmid

    #85982

    Purpose

    Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Inserts

    Cas9
    Urease-targeting gRNA 1
    Urease-targeting gRNA 2
    

    Use

    CRISPR; Diatom (t. pseudonana)

    Tags

    NLS and YFP

    Promoter

    FCP and U6

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-EF1adCas9VP64T2ABsd-W

    Plasmid

    #112919

    Purpose

    Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    dCas9VP64-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human EF1a promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLck-mVenus-C1

    Plasmid

    #84337

    Purpose

    Expresses a mVenus tagged with a membrane localization sequence

    Depositor

    Dorus Gadella

    Article

    van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

    Insert

    Lck (MGCVCSSNPELPVAT)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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