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Plasmid#214324PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationV47N_I48NPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-18_rat
Plasmid#214333PurposeProtein overexpression in bacteriaDepositorInsertIL18 (Il18 Rat)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-BirA-FLAG GFP minimal CMV pCDNA5
Plasmid#212087PurposeLR vector for integration of GFP into N2a FRT rtTA3 expression cells (BioID vector)DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSynW pHluorin-SYT9 IRES mRuby3
Plasmid#195698PurposeLentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorInsertSyt9 (Syt5 Rat)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt9 IRES mRuby3
Plasmid#195700PurposeLentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationencodes only the first 68 amino acids of Tac1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW SYT9 D197, 199N IRES GFP
Plasmid#195701PurposeLentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationfull-length mouse SYT9 with D197, 199N mutationsPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW SYT9 D330, 332N IRES GFP
Plasmid#195702PurposeLentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationfull-length mouse SYT9 with D330, 332N mutationsPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2AB D197,199,330,332N
Plasmid#195706PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutationsDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 104-386 of mouse SYT9 with D1…PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-Best1-Txnip(C247S_LL351-352AA)
Plasmid#206344PurposeAAV plasmid expressing mutant TXNIP in retinal pigment epitheliumDepositorInsertTxnip (Txnip Mouse)
UseAAVTagsExpressionMutationC247S, L351A, L352APromoterhuman Best1Available sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-1 (b)
Plasmid#204429PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
UseTagsEGFPExpressionMammalianMutationE626A T630A K668A E671APromoterCMVAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Palpha6 and Interface-4 (a)
Plasmid#204434PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
UseTagsEGFPExpressionMammalianMutationF860A Y861A Y862A Y863A Q864A R865A H866A F867APromoterCMVAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Palpha2
Plasmid#204441PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
UseTagsEGFPExpressionMammalianMutationW771A K772A K773A R774A W775A L776A Y777A W778A K…PromoterCMVAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA11-pMa-sgTS3-17xMS2V5-sgTS3
Plasmid#199442PurposeCRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology armsDepositorInsertHITI donor template
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Camk2a
Plasmid#186818PurposeFluorescent reporter for Camk2a expressionDepositorInsertCamk2a promoter (Camk2a Mouse)
UseLuciferaseTagsExpressionMutationPromoterCamk2a promoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Camk2b
Plasmid#186819PurposeFluorescent reporter for Camk2b expressionDepositorInsertCamk2b promoter (Camk2b Mouse)
UseLuciferaseTagsExpressionMutationPromoterCamk2b promoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGl3-Camk2g
Plasmid#186821PurposeFluorescent reporter for Camk2g expressionDepositorInsertCamk2g promoter (Camk2g Mouse)
UseLuciferaseTagsExpressionMutationPromoterCamk2g promoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
Plasmid#176310PurposeExpresses CreN59-nMag-NLS with mCherry marker.DepositorInsertCreN59_nMag_NLS_P2A_mCherry_IRES_puroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman EF1alphaAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS-4x(tRNA::axed{sgRNA})
Plasmid#187883PurposeGal4/UAS sgRNA expression targeting axedDepositorInsert4 sgRNAs targeting axed
UseTagsExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-4x(tRNA::hiw{sgRNA})
Plasmid#187884PurposeGal4/UAS sgRNA expression targeting hiwDepositorInsert4 sgRNAs targeting hiw
UseTagsExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-4x(tRNA::dsarm{sgRNA})
Plasmid#187885PurposeGal4/UAS sgRNA expression targeting dsarmDepositorInsert4 sgRNAs targeting dSarm
UseTagsExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABR mCherry-Bcd-LEXY
Plasmid#182596PurposeDrosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertBcd (bcd Fly)
UseTagsLEXY and mCherryExpressionInsectMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Arkadia C946S H948L
Plasmid#180373Purposeexpressing mouse Arkadia (isoform 3) C946S H948L E3 ligase dead mutant proteinsDepositorInsertArkadia (Rnf111 Mouse)
UseRetroviralTagsExpressionMammalianMutationC946S H948LPromoterLTR promoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIN-Skil-gRNA1
Plasmid#180370Purposetargeting mouse Skil/SnoN geneDepositorInsertSkil targeting gRNA (Skil Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIN-Ski-gRNA1
Plasmid#180368Purposetargeting mouse Ski geneDepositorInsertSki targeting gRNA (Ski Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_RalA(G23V,full)-P2A-Hygro_Barcode
Plasmid#170249PurposeBarcoded lentiviral vector to express RalA(G23V, full) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertRALA (RALA Human)
UseLentiviralTagsExpressionMutationG23VPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 ZUC
Plasmid#169964PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND L370G
Plasmid#169970PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationL370GPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND L368G
Plasmid#169969PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (L368G)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationL368GPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM
Plasmid#169975PurposepDONR221 for Gateway shuttling. Contains CARD8 (no stop codon) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPAMPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND R394E
Plasmid#169971PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationR394EPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only