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Halo-TNFa-RUSH

Plasmid

#166901

Purpose

RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-Halo

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

HaloTag

Expression

Mammalian

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-TfR-RUSH

Plasmid

#166905

Purpose

RUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-Halo

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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2xMyc-tdMCP-SNAP (tdMCP standard)

Plasmid

#226166

Purpose

For transient expression of the tdMCP-protein standard. Used in LABEL-seq abundance assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

2xMyc-tdMCP-SNAPtag

Use

Lentiviral

Tags

2xMyc-tdMCP and EGFP-IRES-BlastR

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EBFP_Donor

Plasmid

#178089

Purpose

HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EBFP (T65S/F145Y)

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-PGC1

Plasmid

#4

Depositor

Bruce Spiegelman

Article

Puigserver et al Cell 1998 Mar 20;92(6):829-39.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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fos-tTA

Plasmid

#34856

Depositor

Mark Mayford

Article

Reijmers et al Science. 2007 Aug 31;317(5842):1230-3.

Insert

Fos promoter/first intron -764/+918 (Fos Mouse)

Use

Mouse Targeting

Tags

Fos 3' UTR (~1500bp) and tTA (tetracycline-c…

Expression

Mammalian

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-UBF

Plasmid

#17656

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

UBF (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT

Plasmid

#80951

Purpose

Expresses Gsdmd-NT in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-cIAP1/pRK5

Plasmid

#27972

Depositor

Xiaolu Yang

Article

Hu et al J Biol Chem. 2003 Mar 21;278(12):10055-6

Insert

CIAP1 (BIRC2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only
pEM705 Gsi (Ser)

Plasmid

#109352

Purpose

GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsi chimera

Tags

None

Expression

Mammalian

Mutation

Changed Cys at position 391 to Serine (Pertussis …

Promoter

CAG

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-PE2-C

Plasmid

#164908

Purpose

Expresses the C-terminal split-intein fragment of PE2

Depositor

Wen Xue

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

PE2-C

Use

AAV

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 HSPA6

Plasmid

#19513

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 6 (HSPA6 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn

Plasmid

#159487

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(1)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-EGFP

Plasmid

#107721

Purpose

For expression of sgRNA from the U6 promoter with EGFP expression simultaneously

Depositor

Xingxu Huang

Article

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRGE32

Plasmid

#63159

Purpose

Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

Depositor

Yinong Yang

Article

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Rice snoRNA U3 promoter for gRNA/PTG expression a…

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DMC1

Plasmid

#222541

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DMC1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DMC1 (DMC1 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Gi-c001

Plasmid

#173078

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GNAI1 (GNAI1 Human)

Tags

mCitrine

Expression

Bacterial

Mutation

codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-UBF

Plasmid

#26672

Depositor

Sui Huang

Article

Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

Insert

UBF1 (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
NPM1_mNeonGreen_Donor

Plasmid

#178093

Purpose

HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

NPM1-mNeonGreen HDR donor

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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