We narrowed to 157,076 results for: Des

1. pDEST17-ScChd1(118-1274)

   Plasmid

   #221207

   Purpose

   ScChd1-aa118-1274 chromatin remodeler

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   ScChd1 (aa118-1274)
   

   Tags

   6xHis-tag (Precission protease cleavable)

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-117 and 1275-1468

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT/FLAG/HA-DEST EIF2C2

   Plasmid

   #19888

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   Eukaryotic translation initiation factor 2C, 2 (AGO2 Human)
   

   Tags

   FLAG/HA

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti X2 Zeo DEST (749-3)

   Plasmid

   #21562

   Purpose

   3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR insertion, Zeo

   Depositor

   Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. JN302: pMVP/SB/Puro-DEST

   Plasmid

   #121865

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-DHFR F[1,2]-N (TRP1)

   Plasmid

   #177797

   Purpose

   Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Tags

   DHFR F[1,2]

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. IZ501: pMVP/Lenti-DEST

   Plasmid

   #121848

   Purpose

   pMVP destination vector, empty Lentivirus vector backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Lentiviral; Pmvp gateway destination vector

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LB701: pMVP/PB/mCherry-DEST

   Plasmid

   #121871

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-CMV GFP-GABARAPL1 WT

   Plasmid

   #123117

   Purpose

   Expresses EGFP-GABARAPL1 wild-type in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-GST-FBW7 alpha

   Plasmid

   #197456

   Purpose

   The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 (FBXW7 Human)
   

   Use

   In vitro translation

   Tags

   GST

   Expression

   Bacterial

   Mutation

   See depositor comments below

   Promoter

   T7

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LC101: pMVP/PB/Neo-DEST

    Plasmid

    #121875

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LC301: pMVP/PB/Zeo-DEST

    Plasmid

    #121877

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. 370 pTol2-lmo2:CherryDest

    Plasmid

    #73489

    Purpose

    Tol2 vector with lmo2 promoter upstream of Cherry, attR1/R2 cassette, and late SV40 pA signal

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    lmo2 promoter:mCherry-attR1-attR2
    

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST40-2XFL-Src-S4-S32-KD

    Plasmid

    #140299

    Purpose

    Expresses Flag-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    lacks the UD region

    Promoter

    CMV

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST40-2XFL-Src-S32-KD

    Plasmid

    #140311

    Purpose

    Expresses Flag-tagged human Src-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Contains only the SH3, SH2, and KD regions

    Promoter

    CMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST40-2XHA-Src-Y419F

    Plasmid

    #140316

    Purpose

    Expresses HA-tagged human Src-Y419F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Y419F

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT/FLAG/HA-DEST LIN28A

    Plasmid

    #43797

    Depositor

    Thomas Tuschl

    Article

    Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

    Insert

    LIN28A (LIN28A Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

    Plasmid

    #172535

    Purpose

    Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    -3.5ubb:plin2-tdtomato
    

    Mutation

    S80R, A81S, E144D, I148F and R415H mutations obse…

    Promoter

    zebrafish ubb

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAdCMV/V5-DEST-STAT3C-3xFlag

    Plasmid

    #99264

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Mutation

    A662C N664C

    Promoter

    CMV

    Available Since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRT/FLAG/HA-DEST LIN28B

    Plasmid

    #43798

    Depositor

    Thomas Tuschl

    Article

    Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

    Insert

    LIN28B (LIN28B Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCK033 p5E desma MCS

    Plasmid

    #195948

    Purpose

    Gateway p5E vector

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements (desma Zebrafish)
    

    Use

    Other

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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