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Plasmid#108840PurposeInsert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only
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pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpA
Plasmid#108841PurposeInsert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsPYLExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpA
Plasmid#108847PurposeInsert protein fragment into KpnI/EcoRI to fuse to pMag tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagspMagExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA
Plasmid#108836PurposeInsert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpA
Plasmid#108842PurposeInsert protein fragment into MluI/BspEI to fuse to GAI/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGAI,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1310_pCAG-PV1-NTD-ENTRY-L1-PYL-NLS-BGHpA
Plasmid#108838PurposeInsert protein fragment into MluI/BspEI to fuse to PYL/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsPYL,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA
Plasmid#108839PurposeInsert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsABIExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1049_pCAG-PV1-NTD-ENTRY-L1-FKBP-NLS-BGHpA
Plasmid#108834PurposeInsert protein fragment into MluI/BspEI to fuse to FKBP/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFKBP,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA
Plasmid#108835PurposeInsert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-mIFITM3-deltaY20
Plasmid#58459PurposeExpresses murine IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsmycExpressionMammalianMutationTyrosine 20 deletedPromoterCMV IEAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-mIFITM3-Y20A
Plasmid#58460PurposeExpresses murine IFITM3-Y20A with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsmycExpressionMammalianMutationTyrosine 20 to alaninePromoterCMV IEAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse eIF2C1
Plasmid#50353PurposeMouse eIF2C1 cDNA cloneDepositorInserteIF2C1 (Eif2c1 Mouse)
UseTagsExpressionBacterialMutationPromoterT3Available sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
TAPBPL pEGFP-C3 (1765)
Plasmid#62055Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertTAPBPL (TAPBPL Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s
Plasmid#100020PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.DepositorInsertsfMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2A1
Plasmid#67076PurposeStandard for cell-free expression benchmarking.DepositorInsertGTF2A1 (GTF2A1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-CTRLg2 (CG510)
Plasmid#139456PurposeLentiviral vector with non-targeting gRNA and puromycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW2066_pCAG-PV1-NTD-ENTRY-L1-GID1-NLS-BGHpA
Plasmid#108844PurposeInsert protein fragment into MluI/BspEI to fuse to GID1/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 BTF3
Plasmid#67059PurposeStandard for cell-free expression benchmarking.DepositorInsertBTF3 (BTF3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 SMAD2
Plasmid#67095PurposeStandard for cell-free expression benchmarking.DepositorInsertSMAD2 (SMAD2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA
Plasmid#133347Purposeexpression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)DepositorInsertsgRNA_cpaA and sgRNA_blaA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only