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We narrowed to 7,853 results for: 11

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Showing: 4301 - 4350 of 7853 results

  8. PatoM-LOG241

    Plasmid
    #72847
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. LOG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoM
    Expression
    Bacterial
    Promoter
    PatoM
    Available Since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72847-plasmid-map-sequence-id-133613

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  9. Cx26-sfGFP

    Plasmid
    #69026
    Purpose
    Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to non-monomerized superfolder GFP. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx26 (Gjb2 Rat)
    Tags
    non-monomerized superfolder GFP (V206)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69026-plasmid-map-sequence-id-124714

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  10. MTK0_012

    Plasmid
    #123967
    Purpose
    Encodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    hRosa26-BxBI landing pad
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123967-plasmid-map-sequence-id-251720

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  13. sfGFP-Cx43K258stop

    Plasmid
    #69025
    Purpose
    Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with sfGFP (V206-version) on the N-terminus. CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Tags
    super folder GFP
    Expression
    Mammalian
    Mutation
    non-monomerized sfGFP fused in frame to N-terminu…
    Promoter
    CMV
    Available Since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69025-plasmid-map-sequence-id-127523

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  19. JPF0522

    Plasmid
    #124013
    Purpose
    Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124013-plasmid-map-sequence-id-252424

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  21. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid
    #84631
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    ERKsub(TA)DD-Venus-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Mutated target Thr residue to Ala.
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    84631-plasmid-map-sequence-id-175151

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  28. pClneoFH-let60 G12V

    Plasmid
    #66860
    Purpose
    Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells
    Depositor
    Yutaka Hata
    Article
    Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do
    Insert
    Let-60 (let-60 Nematode)
    Tags
    FLAG-His6
    Expression
    Mammalian
    Mutation
    Glycine 12 is mutated to valine
    Promoter
    CMV
    Available Since
    Aug. 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  29. MTK0_001

    Plasmid
    #123924
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    TU-sgRNA - L1/RE
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123924-plasmid-map-sequence-id-250078

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  39. PatoG-OG241

    Plasmid
    #72839
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoG
    Expression
    Bacterial
    Promoter
    PatoG
    Available Since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72839-plasmid-map-sequence-id-133603

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  42. pLJSRSF7-T7-6xHis-SUMO-MRT-CBD

    Plasmid
    #253350
    Purpose
    For the expression of group II intron encoded reverse transcriptase MarathonRT with N-terminal SUMO-tag and C-terminal CBD-tag in E. coli.
    Depositor
    Peter Sarin
    Article
    Pedor et al Bio-protocol 2026, 16(11): e5703. DOI: 1
    Insert
    6xHis-SUMO-MarathonRT with added C-terminal CBD-tag originating from the backbone.
    Tags
    6xHis-SUMO and CBD
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    253350-plasmid-map-sequence-id-507308

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  43. pM3_PLux*R1CI*_PLTetO1R1CI*_PLCR1E2Cr_PJ23102R1LuxR

    Plasmid
    #250890
    Purpose
    LuxR expressed under BBa_J23102; E2-Crimson under PLC promoter; mutated cI* under PLTetO1; and cI* under Plux*; part of BNeuAS5; pUC origin of replication
    Depositor
    Sangram Bagh
    Article
    Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534
    Inserts
    E2-Crimson
    LuxR
    Mutated cI
    Mutated cI
    Use
    Synthetic Biology
    Mutation
    Frame shifted cI protein and Frame shifted cI pro…
    Promoter
    BBa_J23102, PLC, PLTetO1, and Plux
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    250890-plasmid-map-sequence-id-502190

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Showing: 4301 - 4350 of 7853 results