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We narrowed to 4,416 results for: Pet2

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Showing: 4301 - 4350 of 4416 results

  5. NmMetQ C20A

    Plasmid
    #172112
    Purpose
    Expression of Methionine Binding Protein (MetQ) with C20A mutation in signal sequence
    Depositor
    Douglas Rees
    Article
    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:
    Insert
    Methionine Binding Protein
    Tags
    10x Histidine Tag and N.meningitidis signal seque…
    Expression
    Bacterial
    Mutation
    Changed Proline 22 to Alanine on MetQ and Cystein…
    Promoter
    T7 Promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172112-plasmid-map-sequence-id-344822

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  7. hzHDAC10

    Plasmid
    #225542
    Purpose
    Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.
    Depositor
    David Christianson
    Article
    Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21
    Insert
    Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Mutation
    Mutated A24E and D94A
    Promoter
    T7
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225542-plasmid-map-sequence-id-448447

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  12. Pdpy-30-miniCAFE

    Plasmid
    #170115
    Purpose
    Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.
    Depositor
    Zhili Rong
    Article
    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617
    Insert
    MiniCAFE
    Use
    CRISPR
    Mutation
    D8A, HNH-truncation (Δ495–609 aa) in CjCas9
    Promoter
    Dpy-30
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170115-plasmid-map-sequence-id-336055

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  15. pET-EIN-AlkBH5(66-292)

    Plasmid
    #125732
    Purpose
    Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.
    Depositor
    Vincenzo Venditti
    Article
    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055
    Insert
    AlkBH5(66-292) (ALKBH5 Human)
    Tags
    6xHis and EIN
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125732-plasmid-map-sequence-id-244619

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  16. pBJG1 T7-8xHis-OGT K842M

    Plasmid
    #154272
    Purpose
    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Lysine 842 changed to Methionine- bacterial codon…
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154272-plasmid-map-sequence-id-304833

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  17. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

    Plasmid
    #62373
    Purpose
    Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells
    Depositor
    David Liu
    Article
    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do
    Insert
    (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
    Tags
    (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…
    Expression
    Bacterial
    Mutation
    Y67S amino acid substitution in (-30)GFP renders …
    Promoter
    T7
    Available Since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    62373-plasmid-map-sequence-id-263816

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  23. pBJG1 T7-8xHis-OGT D554N

    Plasmid
    #154279
    Purpose
    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Aspartate 554 changed to Asparagine- bacterial co…
    Available Since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154279-plasmid-map-sequence-id-304834

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  33. His6-SUMO-SPMc-SpyCatcher003-Ctag

    Plasmid
    #246648
    Purpose
    Expresses His6-SUMO-SPMc-SpyCatcher003-Ctag in bacterial cells for calcium-induced NeissLock protein ligation
    Depositor
    Mark Howarth
    Article
    Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021
    Insert
    His6-SUMO-SPMc-SpyCatcher003-Ctag
    Tags
    Ctag, His-tag, SUMO, and SpyCatcher003
    Expression
    Bacterial
    Mutation
    The SPMc comprises the C-terminal fragment of th…
    Promoter
    T7
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    246648-plasmid-map-sequence-id-491284

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  35. Ce iPGM-C-HiBiT

    Plasmid
    #207120
    Purpose
    Bacterially expressed iPGM-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    C. elegans-iPGM HiBIT His10 (ipgm-1 Nematode)
    Tags
    HiBiT Tag and Thrombin cleavage site + 10x His Ta…
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207120-plasmid-map-sequence-id-417169

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  36. E. coli Lig-N-HiBiT

    Plasmid
    #215437
    Purpose
    Bacterially expressed E. coli Lig-N-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    NAD-dependent DNA ligase LigA [Escherichia coli], N-HiBiT, N-10XHis.Thrombin
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215437-plasmid-map-sequence-id-428719

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  37. E. coli Lig-C-HiBiT

    Plasmid
    #215438
    Purpose
    Bacterially expressed E. coli Lig-C-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    NAD-dependent DNA ligase LigA [Escherichia coli], C-HiBiT, C-10XHis.Thrombin
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215438-plasmid-map-sequence-id-428720

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Showing: 4301 - 4350 of 4416 results