We narrowed to 11,348 results for: Mpl
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Plasmid#204291PurposeFor inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.DepositorInsertie1-DsRed
UseCRISPRPromoterie1Available SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACYC-TtCas6-4xcrRNA4.5
Plasmid#127764PurposeFor expression of T. thermophilus Csm complex in E. coli (crRNA)DepositorInsertsSynthetic CRISPR array containing four identical T. thermophilus spacers
Cas6A
ExpressionBacterialMutationCodon optimized for expression in E. coliPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO CBX1-APEX2
Plasmid#129642PurposeExpress heterochromatin-localized CBX1-APEX2 fusion in mammalian cellsDepositorAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
CD55-bio-His
Plasmid#53152PurposeExpresses full-length CD55 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCD55 (CD55 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL
Plasmid#111389PurposeAAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsChETA-mRuby2
W3SL
UseAAVTagsmRuby2ExpressionMammalianMutationW3SL is built from a shortened WPRE, an upstream …PromoterhSynapsinAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAT17
Plasmid#204298PurposeFor introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.DepositorInsert3XP3-EYFP
UseCRISPRPromoter3XP3Available SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-eGFP-FHA-SHLD2
Plasmid#114121PurposeLentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatinDepositorInsertSHLD2 (SHLD2 Human)
UseLentiviral; Doxycycline inducibleTagsRNF8 FHA domain (aa 2-160) and eGFPExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
His-NavMs
Plasmid#100004PurposeElectrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFPDepositorInsertHis-NavMs, Voltage gated sodium channel
TagsHexa-His tag followed by a thrombin cleavage siteExpressionMammalianMutationC-terminus from a codon-optimized synthetic gene,…PromoterCMVAvailable SinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag Depdc5 pLJM1
Plasmid#46336DepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsFlagExpressionMammalianMutationDiffers from Depdc5 isoform 1 by 10 amino acids …Available SinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS6-ZF(VEGFA)-StaPL(AI)-YFP-VPR
Plasmid#111502PurposeExpresses a zinc finger specific to the human VEGFA locus, which is linked to a VPR transcriptional activation domain via a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].DepositorInsertZF(VEGFA)-StaPL(AI)-YFP-VPR
ExpressionMammalianMutationThe HCV NS3 protease carries V36M, T54A, and S122…PromoterCMVAvailable SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLC-255
Plasmid#124292PurposeExpression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
ExpressionBacterialAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pet-MBP
Plasmid#209184PurposeExpression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coliDepositorTypeEmpty backboneTagsHisX6ExpressionBacterialPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z23
Plasmid#120066PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with HaloDepositorInsertHalo
UsePcr templateTagsHaloPromoterNoneAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P06
Plasmid#120017PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with sfGFPDepositorInsertsfGFP
UsePcr templateTagssfGFPPromoterNoneAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Venus1-WTSTAT3
Plasmid#123164PurposeExpresses wild type STAT3 fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
TagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationnonePromoterpCMVAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4c/His-Xpress-HA-hBIG1(E793K)
Plasmid#79433PurposeExpresses N-terminally His6, Xpress and HA-tagged BIG1(E793K) in mammalian cellsDepositorInsertBIG1 (ARFGEF1 Human)
TagsHA, His6, and XpressExpressionMammalianMutationchanged Glutamic acid 793 to LysineAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-o-YFP
Plasmid#55776PurposeThis G protein alpha-o construct contains internal insertions of YFP and the EE epitopeDepositorInsertG protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
TagsEE epitope was introduced internally with D167E a…ExpressionMammalianMutationA BglII site was removed from the 5' untrans…PromoterCMVAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJZC42
Plasmid#66564PurposesgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherryDepositorInsertssgRNA + 1XPP7
PCP-VP64 IRES mCherry
ExpressionMammalianPromoterCMV and U6Available SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
A1-20B1(P004)
Plasmid#184056PurposeThis plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.DepositorInsertA1-20B1 preamplifier
UseSynthetic BiologyAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only