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We narrowed to 7,223 results for: ALP

Showing: 4351 - 4400 of 7223 results

  1. pX551-miniCMV-SpCas9D10A nickase

    Plasmid
    #216735
    Purpose
    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    T7 and mini-CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216735-plasmid-map-sequence-id-430321

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  5. pVRC8400-SARS-CoV-2-RBD-SD1-B.1.1.529-AVI

    Plasmid
    #181704
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.1.529 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181704-plasmid-map-sequence-id-354499

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  8. pVRC8400-SARS-CoV-2-RBD(aa330-526)-B.1.1.529-AVI

    Plasmid
    #182930
    Purpose
    Expresses SARS-CoV-2 RBD (aa324-526) domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD(aa324-526)-B.1.1.529 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …
    Promoter
    Available since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    182930-plasmid-map-sequence-id-358143

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  9. pVRC8400-SARS-CoV-2-RBD(aa335-526)-B.1.1.529-AVI

    Plasmid
    #181701
    Purpose
    Expresses SARS-CoV-2 RBD domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-B.1.1.529 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181701-plasmid-map-sequence-id-354500

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  11. CBP core-dCas9-CBP core

    Plasmid
    #179560
    Purpose
    encodes S. pyogenes dCas9 with both n-terminal and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with both n-terminal and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    179560-plasmid-map-sequence-id-365385

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  15. Myc-gamma2/deltaILTM4

    Plasmid
    #119735
    Purpose
    GABAA receptor expression (chimeric rat rat gamma 2 subunit whose large intracellular loop, TM4 and C-term have been replaced with the equivalent domains of the rat delta subunit) C-myc near N-term
    Depositor
    Angel de Blas
    Article
    Wu et al J Biol Chem. 2012 Aug 10;287(33):27417-3
    Insert
    GABRG2/GABRD (Gabrg2, Gabrd Rat)
    Use
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    119735-plasmid-map-sequence-id-229652

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  17. pVRC8400-SARS-CoV-2-NTD-B.1.1.529-AVI

    Plasmid
    #181698
    Purpose
    Expresses SARS-CoV-2 NTD domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-NTD-B.1.1.529 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    A67V, ∆H69, ∆V70, T95I, G142D, ∆V143, ∆Y144, ∆Y14…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181698-plasmid-map-sequence-id-354496

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  18. pCMVtrunc msfGFPΔC10-Utr28-222

    Plasmid
    #231558
    Purpose
    Expresses N- and C-terminally truncated Utrophin calponin homology domain fused to C-terminally truncated msfGFP for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Utrophin calponin homology domain (UTRN Human)
    Use
    Tags
    msfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMVtrunc
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231558-plasmid-map-sequence-id-459698

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  22. pVRC8400-SARS-CoV-2-RBD-BQ.1.1-AVI

    Plasmid
    #196618
    Purpose
    Expresses SARS-CoV-2 RBD protein from the BQ.1.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-BQ.1.1
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, R346T, S371F, S373P, S375F, T376A, D405N, …
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196618-plasmid-map-sequence-id-385182

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  23. pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)

    Plasmid
    #192704
    Purpose
    Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Use
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPm…
    Expression
    Mammalian
    Mutation
    meGFPm: gluatmic acid 142 to asparagine and histi…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192704-plasmid-map-sequence-id-379659

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  24. pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

    Plasmid
    #192784
    Purpose
    Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Use
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192784-plasmid-map-sequence-id-379372

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  27. pVRC8400-SARS-CoV-2-RBD-SD1-BA.2-AVI

    Plasmid
    #184534
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-SD1-BA.2 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …
    Promoter
    Available since
    June 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    184534-plasmid-map-sequence-id-364434

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  28. pVRC8400-SARS-CoV-2-S2P-BA.2-AVI

    Plasmid
    #184531
    Purpose
    Expresses SARS-CoV-2 S2P protein from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-BA.2 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19I, L24-, P25-, P26-, A27S, G142D, V213G, G339D…
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    184531-plasmid-map-sequence-id-369456

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  29. pVRC8400-SARS-CoV-2-RBD-SD1-K417N-L452R-T478K-AVI

    Plasmid
    #176457
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain containing K417N-L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-SD1-K417N-L452R-T478K (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    K417N, L452R, T478K
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176457-plasmid-map-sequence-id-347378

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  30. pVRC8400-SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y-AVI

    Plasmid
    #176450
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain containing K417N-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176450-plasmid-map-sequence-id-347372

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  31. pVRC8400-SARS-CoV-2-RBD-SD1-K417T-E484K-N501Y-AVI

    Plasmid
    #176451
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain containing K417T-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-SD1-K417T-E484K-N501Y (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    K417T, E484K, N501Y
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176451-plasmid-map-sequence-id-347374

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  32. pVRC8400-SARS-CoV-2-S2P-B.1.617-E154K-L452R-E484Q-D614G-P681R-AVI

    Plasmid
    #176331
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.617 variant containing E154K-L452R-E484Q-D614G-P681R mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.617 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    E154K, L452R, E484Q, D614G, P681R
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176331-plasmid-map-sequence-id-347140

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  33. GFP-Cstem(7A)-Tac(5A)

    Plasmid
    #162499
    Purpose
    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A).
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    CD8a stem region with mutations and Tac full length with mutated stem region (IL2RA, CD8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five theronine and two serine residues are mu…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162499-plasmid-map-sequence-id-323491

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  37. p300 core-dCas9-CBP core

    Plasmid
    #179559
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) & c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179559-plasmid-map-sequence-id-354771

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  38. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168123-plasmid-map-sequence-id-332865

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  39. pGP-AAV-syn-FLEX-Volton2-ST-WPRE

    Plasmid
    #172907
    Purpose
    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence
    Depositor
    GENIE Project
    Article
    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-
    Has Service
    AAV1
    Insert
    Voltron2-ST
    Use
    AAV and Cre/Lox
    Tags
    soma localization targeting sequence
    Expression
    Mammalian
    Mutation
    A122D
    Promoter
    hSynapsin1
    Available since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    172907-plasmid-map-sequence-id-353194

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  42. pQ-Elo-HB-C-A(1-630)

    Plasmid
    #171086
    Purpose
    Co-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26aa and C-term 142aa for mutant e…
    Promoter
    T5 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171086-plasmid-map-sequence-id-335555

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  45. GCN5 core-dCas9-p300 core

    Plasmid
    #179553
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179553-plasmid-map-sequence-id-354783

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  46. pVRC8400-SARS-CoV-2-RBD-XBB.1.5-AVI

    Plasmid
    #198466
    Purpose
    Expresses SARS-CoV-2 RBD protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-XBB.1.5 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339H, R346T, L368I, S371F, S373P, S375F, T376A, …
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198466-plasmid-map-sequence-id-388113

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  48. pQ-Elo-HB-C-A(400-772)

    Plasmid
    #171087
    Purpose
    Co-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26+399 amino acids for mutant expr…
    Promoter
    T5 promoter and T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171087-plasmid-map-sequence-id-335508

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  49. pQ-Elo-HB-C-A(1-772)

    Plasmid
    #171085
    Purpose
    Co-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26 amino acids for expression purp…
    Promoter
    T5 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171085-plasmid-map-sequence-id-335501

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Showing: 4351 - 4400 of 7223 results