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YY229: pAAV_TRE::GFP- GEMINI(A)-P1N4_hSyn::rtTA3G

Plasmid

#228889

Purpose

AAV plasmid expressing the destabilized GFP-GEMINI(A) under the control of a doxycycline-inducible promotor, and rtTA3G under the control of a hSyn promotor

Depositor

Dingchang Lin

Article

Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-

Insert

GFP-GEMINI(A); rtTA3G

Use

AAV

Tags

gfp

Promoter

TRE; hsyn

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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PLX306 iCre tetO tomato rtTA

Plasmid

#158050

Purpose

lenti-viral construct with Cre recombinase and doxycycline inducible expression of tdTomato

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

tdTomato

Tags

none

Expression

Mammalian

Promoter

tetO promoter

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-ZNF281

Plasmid

#222923

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

ZNF281 (ZNF281 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLVXT-mTcf3

Plasmid

#121935

Purpose

doxycycline-inducible mouse Tcf3

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Transcription Factor 3 (Tcf3 Mouse)

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PB-Lmo2

Plasmid

#60666

Purpose

PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Lmo2 (Lmo2 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_TRE-mScarlet

Plasmid

#229796

Purpose

Bxb1-GT donor plasmid for doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mScarlet

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCarCBAE

Plasmid

#117755

Purpose

Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE

Expression

Bacterial

Promoter

PLac(UV5)

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-cyclin E

Plasmid

#216508

Purpose

Expresses cyclin E1 in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CCNE1 (CCNE1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTS101 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOEu-MCS

Plasmid

#199354

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOEu-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-SUMOEu

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Olig2

Plasmid

#64842

Purpose

A lentiviral vector for the expression of mouse Olig2 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Olig2 (Olig2 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-CDC25A

Plasmid

#216511

Purpose

Expresses CDC25A in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CDC25A (CDC25A Human)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TRE3G-SOX17

Plasmid

#104541

Purpose

PiggyBac vector encoding doxycycline-inducible human SOX17

Depositor

David Vereide

Article

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

SRY-box 17 (SOX17 Human)

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-HOXA9

Plasmid

#51302

Purpose

Expression of HOXA9 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

homeobox A9 (HOXA9 Human)

Use

Lentiviral

Tags

n/a

Mutation

n/a

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pEasiLV-MCS-coICP0

Plasmid

#235545

Purpose

To express ICP0 (doxycycline inducible)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ICP0

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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XLone-SOX18

Plasmid

#163822

Purpose

expresses human SOX18 upon doxycycline treatment

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

SOX18 (SOX18 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9GFP-IRES-Neo

Plasmid

#102907

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTS113 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-Alfa-P2A-TagBFP

Plasmid

#199366

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOEu-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-puro_TAOK3

Plasmid

#209778

Purpose

doxycycline-inducible expression of TAOK3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

TAOK3 (TAOK3 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TetON-Bmpr1A-ca

Plasmid

#217536

Purpose

AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

constitutively active BMP receptor 1a (BMPR1A Human)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

Tet operon (TetO)

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pTS100 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-TEV-MCS

Plasmid

#199353

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-TEV-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-TEV

Expression

Mammalian

Mutation

WT

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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