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pFA6a-GFP(S65T)-His3MX6

Plasmid

#41598

Depositor

John Pringle

Publication

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Yeast

Promoter

TEF

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26

Plasmid

#92143

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSUMO2

Plasmid

#52259

Purpose

Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-2 (SUMO2 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Flag-HA-EIF3S3

Plasmid

#22545

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

EIF3S3(1-348aa) (EIF3H Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST17-UbE2D3

Plasmid

#15784

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D3 (UBE2D3 Human)

Tags

His

Expression

Bacterial

Mutation

see Depositor Comments below

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Flag-Daxx/pRK5

Plasmid

#27974

Depositor

Xiaolu Yang

Publication

Tang et al Nat Cell Biol. 2006 Aug . 8(8):855-62.

Insert

Daxx (DAXX Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pLCRcala (p53)

Plasmid

#12136

Depositor

James Sherley

Publication

Sherley et al J Biol Chem. 1991 Dec 25. 266(36):24815-

Insert

p53 (Trp53 Mouse)

Expression

Mammalian

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pEF-Bos-FLAG RIG-I MIII

Plasmid

#52877

Purpose

Expresses RIG-I with a mutation to motif III in mammalian cells

Depositor

Curt Horvath

Publication

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

DDX58 (RIGI Human)

Tags

FLAG

Expression

Mammalian

Mutation

T409A, S411A

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFosCreER

Plasmid

#46388

Depositor

Liqun Luo

Publication

Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

Inserts

Fos (Fos Mouse)
CreERT2

Use

Mouse Targeting

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HA-FOXO3a WT-ER

Plasmid

#8355

Depositor

Michael Greenberg

Publication

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

mouse estrogen receptor (G525R) on C-term

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MBP-hnRNPA2_FL_WT

Plasmid

#98662

Purpose

Expresses MBP-tagged full length hnRNPA2

Depositor

Nicolas Fawzi

Publication

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBECAb-apr

Plasmid

#122001

Purpose

A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

Depositor

Quanjiang Ji

Publication

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Type

Empty backbone

Use

CRISPR

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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human MMP-9 promoter

Plasmid

#53434

Purpose

promoter activity assay

Depositor

Etty Benveniste, Douglas Boyd

Publication

Ma et al J Immunol. 2001 Nov 1;167(9):5150-9.

Insert

MMP-9 promoter (MMP9 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

MMP9

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PB-TAC-OSKM

Plasmid

#80481

Purpose

piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Publication

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pdCas9-DNMT3A-PuroR (ANV)

Plasmid

#71684

Purpose

Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA

Depositor

Vlatka Zoldos

Publication

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, Human, S. pyogenes)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

CBh

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

Plasmid

#134858

Purpose

Expresses EGFP-tagged early endosome marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Ras-related protein 5A

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCI-neo-hApg5-HA

Plasmid

#22948

Depositor

Noboru Mizushima

Publication

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)

Tags

3xHA

Expression

Mammalian

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles
pCAG G-CEPIA1er

Plasmid

#105012

Purpose

ER calcium sensor

Depositor

Franck Polleux

Publication

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

G-CEPIA1er

Expression

Mammalian

Promoter

pCAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pME phluorin2-gpi

Plasmid

#73793

Purpose

ratiometric pHluorin2 with a gpi linkage sequence in a middle entry clone

Depositor

David Raible

Publication

Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

Insert

ratiometric pHluorin2-gpi

Use

Middle entry clone

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

Plasmid

#115268

Purpose

Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

EiCsm6

Tags

SUMO

Expression

Bacterial

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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