We narrowed to 22,307 results for: CRISPR

1. pLenti-dSaCas9-KRAB-blast

   Plasmid

   #191654

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS SK mCherryROSAbsr

   Plasmid

   #54321

   Purpose

   Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

   Depositor

   Silvestro Conticello

   Article

   Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

   Insert

   mCherry and BlasticidinS-resistance
   

   Use

   CRISPR, Mouse Targeting, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAdSh.U6.gRNAGFP

   Plasmid

   #58255

   Purpose

   U6 promoter-driven single guide RNA complementary to eGFP

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAGFP cassette
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBY3_ligD-HygR

   Plasmid

   #104625

   Purpose

   CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.

   Depositor

   Sheng Yang

   Article

   Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

   Insert

   FnCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   FnCpf1 codon-optimized C.glutamicum

   Promoter

   hsp60

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA1_Tet-inducible Luciferase reporter

   Plasmid

   #64161

   Purpose

   Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1 for Tet-inducible Luciferase reporter
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179051

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolus

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-RPC19-Link-Scarlet(rfp)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LbCas12a

   Plasmid

   #188493

   Purpose

   Expresses FLAG tagged LbCas12a

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2CT-His-MBP-Lbu_C2c2_R1079A

   Plasmid

   #83494

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R1079A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R1079A

   Promoter

   T7

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSMVP-Cas9C-P2A-turboGFP

   Plasmid

   #80935

   Purpose

   Plasmid that expresses Cas9C in mammalian cells; co-expresses turboGFP.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA3_GAL4UAS-Luciferase reporter

    Plasmid

    #64159

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA3 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZeo-Cas9-gate

    Plasmid

    #113744

    Purpose

    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    Cas9
    

    Use

    CRISPR; Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Z. Mays ubiquitin promoter, 35S promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSAG1::CAS9-U6::sg290860-6

    Plasmid

    #59855

    Purpose

    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

    Depositor

    David Sibley

    Article

    Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

    Insert

    CRISPR sg290860-6
    

    Use

    CRISPR; ; toxoplasma gondii

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbdCas9S_Psyn-sgRNA500

    Plasmid

    #149640

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b-NmCas9-Flag

    Plasmid

    #109030

    Purpose

    Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC155-pCR8-sgExpression

    Plasmid

    #49045

    Purpose

    U6-driven sgRNA expressing cassette on a gateway donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAdSh.U6.gRNAS1

    Plasmid

    #58252

    Purpose

    U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    U6.gRNAS1 cassette
    

    Use

    Adenoviral and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFYF1327 EGFP Site#2

    Plasmid

    #47512

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

    Plasmid

    #83484

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R1048A_H1053A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R1048A, H1053A

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. U6-spTRE3G-CMV-mTagBFP2

    Plasmid

    #102854

    Purpose

    A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-TRE3G-sgRNA; mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBK83 pHomL-RPL18Bp-ERG6-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179067

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Scarlet(rfp), a marker for the lipid droplet

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-ERG6-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBBK82 pHomL-RPL18Bp-Scarlet(rfp)-Link-ATG8-SSA1t-HomR (AmpR)

    Plasmid

    #179066

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-ATG8, a marker for the autophagosome

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-ATG8
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBBK76 pHomL-RPL18Bp-PIL1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179060

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Scarlet(rfp), a marker for the eisosomes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-PIL1-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBBK70 pHomL-RPL18Bp-Scarlet(rfp)-Link-TUB1-SSA1t-HomR (AmpR)

    Plasmid

    #179054

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-TUB1, a marker for the microtubules

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-TUB1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. Oct4-Cr1

    Plasmid

    #66989

    Purpose

    Expresses gRNA that targets stop codon for OCT4

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

    Insert

    gRNA for Crispr targeting the OCT4 stop codon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

25. Sox1/2/3-2373/+3>nls::Cas9::nls

    Plasmid

    #59989

    Purpose

    Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Sox1/2/3 (SoxB1) -2373/+3

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. p2CT-His-MBP-Lbu_C2c2_R472A_H477A

    Plasmid

    #83483

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBBK51 pHomL-RPL18Bp-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

    Plasmid

    #179035

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SCS2tmh, a marker for the endoplasmic reticulum

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Neon(gfp)-Link-SCS2tmh
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PX458_NFIL3_2

    Plasmid

    #86349

    Purpose

    Encodes gRNA for 3' target of human NFIL3

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against NFIL3 (NFIL3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PX458_NFIL3_1

    Plasmid

    #86348

    Purpose

    Encodes gRNA for 3' target of human NFIL3

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against NFIL3 (NFIL3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBBK86 pHomL-RPL18Bp-preCOX4-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179070

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Scarlet(rfp), a marker for the mitochondria

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-preCOX4-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge