We narrowed to 4,857 results for: Mela

1. pHD-T2A-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199204

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[21-881]-C-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti6/V5-DEST-ASF1B

   Plasmid

   #31205

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   ASF1B (ASF1B Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

3. 20XUAS IVS ChrimsonR mCherry_tr

   Plasmid

   #111546

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR_mCherry_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJC-repo1.9kb-TALE4-VP64-P10

   Plasmid

   #104612

   Purpose

   Expresses TALE4 under the control of a 1.9kb repo enhancer element

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   1.9kb repo enhancer element (repo Fly)
   

   Use

   TALEN

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available Since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. VP-FKBP51

   Plasmid

   #89097

   Purpose

   mammalian expression of FKBP5 also referred to as FKBP51 with N-terminal VP16 activation domain

   Depositor

   Elizabeth Wilson

   Article

   Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

   Insert

   FKBP5 (FKBP5 Human)
   

   Use

   Tags

   FLAG and VP16

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SiriusGFP

   Plasmid

   #123201

   Purpose

   We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76

   Insert

   SiriusGFP
   

   Use

   Mouse Targeting

   Tags

   N/A

   Expression

   Bacterial and Mammalian

   Mutation

   eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-Y1175F-VEGFR2-mCherry

   Plasmid

   #139608

   Purpose

   Expresses a mutant (Y1175F) VEGFR2-mCherry fusion protein in mammalian cells

   Depositor

   Michael Welsh

   Article

   Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

   Insert

   KDR (KDR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBABR IRFP-Bcd-LEXY

   Plasmid

   #182595

   Purpose

   Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

   Depositor

   Jared Toettcher

   Article

   Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

   Insert

   Bcd (bcd Fly)
   

   Use

   Tags

   IRFP and LEXY

   Expression

   Insect

   Mutation

   Promoter

   maternal tubulin

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. His Puc (aa 1-393)

   Plasmid

   #52295

   Purpose

   Bacterial expression of His tagged Drosophila Puc fragment

   Depositor

   Brenda Schulman

   Article

   Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

   Insert

   Puc (puc Fly)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pCDF1-PTPRD-G446E

    Plasmid

    #25643

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

    Insert

    Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    tumor-derived mutation created by site-directed m…

    Promoter

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

11. 10XUAS IVS ChrimsonR mCherry_tr

    Plasmid

    #111547

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR_mCherry_trafficked
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-Y951F-VEGFR2-mCherry

    Plasmid

    #139607

    Purpose

    Expresses a mutant (Y951F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL410-BRN2p

    Plasmid

    #110733

    Purpose

    Luciferase reporter for the human BRN2 promoter

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    BRN2 promoter luciferase (POU3F2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

    Plasmid

    #199197

    Purpose

    Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int-BlastR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. VP-ARF

    Plasmid

    #89122

    Purpose

    mammalian expression of p14-hARF linked to VP16 activation domain

    Depositor

    Elizabeth Wilson

    Article

    Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

    Insert

    p14-hARF (CDKN2A Human)
    

    Use

    Tags

    VP16

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAFW-Ago2

    Plasmid

    #50554

    Purpose

    Expression of FLAG tagged Ago2 in S2 cell

    Depositor

    Yukihide Tomari

    Article

    Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

    Insert

    Argonaute2 (AGO2 Fly)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

    Plasmid

    #113039

    Purpose

    AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCrysβ:ECFP-LexOP:mCherry-HrasG12V

    Plasmid

    #89887

    Purpose

    Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-HRasG12V
    

    Use

    Unspecified

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-Y1214F-VEGFR2-mCherry

    Plasmid

    #139609

    Purpose

    Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

    Plasmid

    #89886

    Purpose

    Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-NRasQ61K
    

    Use

    Unspecified

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSUPER-retro-puro-shOrai1

    Plasmid

    #89933

    Purpose

    Knockdown human Orai1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    Orai1 shRNA (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUAST-attB-3xHA-V5-RpL10Ab

    Plasmid

    #125223

    Purpose

    3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

    Depositor

    Herman Dierick

    Article

    Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

    Insert

    RpL10Ab (RpL10Ab Fly)
    

    Use

    Tags

    3x HA, V5

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDropIn-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199196

    Purpose

    Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAc-y1sgRNA-Cas9

    Plasmid

    #49331

    Purpose

    Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    y1 sgRNA (y Fly)
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLNCX2-STIM1

    Plasmid

    #89817

    Purpose

    Express HUMAN STIM1

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    NO TAG

    Expression

    Mutation

    Promoter

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSUPER-retro-puro-shSTIM1

    Plasmid

    #89816

    Purpose

    Knockdown human STIM1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    STIM1 shRNA (STIM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL3-DICER-Prom

    Plasmid

    #25851

    Depositor

    David E. Fisher

    Article

    Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

    Insert

    Dicer promoter (DICER1 gene ID 23405, Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLVX-Phi3-Zsgreen-PGK-mCherry

    Plasmid

    #153320

    Purpose

    Expresses zsGreen and mCherry in mammalian cells.

    Depositor

    Robert Judson-Torres

    Article

    McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

    Insert

    zsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. KHBD00476

    Plasmid

    #39585

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5838 (Dref Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

30. UAS-dsarm(E)

    Plasmid

    #187870

    Purpose

    Gal4/UAS expression of dSarm(isoE)

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    dSarm isoform E (Sarm Fly)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GAL-FKBP51

    Plasmid

    #89132

    Purpose

    mammalian expression of FKBP5 also referred to as FKBP51 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    FKBP5 (FKBP5 Human)
    

    Use

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

    Plasmid

    #79259

    Purpose

    Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

    Insert

    DmeIF4G (eIF4G1 Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    V5-His6 and lambdaN-HA

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. prk5-MFSD12-TEV-3xFLAG-3xHA

    Plasmid

    #149504

    Purpose

    Transient expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Transient expression

    Tags

    TEV-3XHA-3XFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized Sequence

    Promoter

    CMV

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Hy_pMT Laccase2 Exons 1-3

    Plasmid

    #91799

    Purpose

    Drosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

    Insert

    Laccase2 (stw Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAc-GFP-dSUMO

    Plasmid

    #85696

    Purpose

    expression of GFP-SUMO1 in Drosophila cells

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

36. YW249_Pzipt17-loxP-H2BGFP-miniMOS

    Plasmid

    #196063

    Purpose

    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

    Depositor

    Wenxing Yang

    Article

    Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

    Inserts

    H2B::GFP
    NeoR
    Minimal Mos1
    zipt17p::zipt17::zipt17 3UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTYF-PRSx8-AstR-p2A-GFP

    Plasmid

    #159632

    Purpose

    Lentiviral expression of the Allatostatin receptor, P2A GFP under the PRSx8 promoter

    Depositor

    Andrew Allen

    Article

    Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

    Insert

    AstR (AstA-R1 Fly)
    

    Use

    Lentiviral

    Tags

    GFP and P2A

    Expression

    Mammalian

    Mutation

    Promoter

    PRSx8

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRSFDuet-1 IntS6 AA 1035-1284

    Plasmid

    #196904

    Purpose

    Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 6 (IntS6 Fly)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CAG-Brn2-DBD-EnR

    Plasmid

    #19714

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    Fly engrailed 36240 (Pou3f2, en Fly, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mouse Brn2 DNA binding domain-Drosophila engraile…

    Promoter

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

40. KHBD00044

    Plasmid

    #34206

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG17117 (hth Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only