We narrowed to 27,841 results for: UNK

1. pMT001

   Plasmid

   #64758

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a V5 epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLNHA-C1-HseIF4A3_I

   Plasmid

   #146578

   Purpose

   Mammalian Expression of HseIF4A3

   Depositor

   Elisa Izaurralde

   Article

   Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

   Insert

   HseIF4A3 (EIF4A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1 human A3C

   Plasmid

   #105047

   Purpose

   Overexpression of APOBEC3C in mammalian cell lines

   Depositor

   Carsten Münk

   Article

   Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

   Insert

   APOBEC3C (A3C) (APOBEC3C Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSD103D

   Plasmid

   #218134

   Purpose

   For competition during selection with hybrid circuit. Expresses MBP fused to evolved degron SD55

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD55
   

   Use

   Tags

   MBP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJM189A

   Plasmid

   #218135

   Purpose

   For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to ZF degron SD0.

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD0
   

   Use

   Tags

   rpoZ

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry

   Plasmid

   #101282

   Purpose

   Lentiviral expression of shRNA and mouse E-cadherin -mCherry

   Depositor

   Alpha Yap

   Article

   Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

   Insert

   E-cadherin
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT7-EGFP-C1-HseIF4A3_I

   Plasmid

   #146581

   Purpose

   Mammalian Expression of HseIF4A3

   Depositor

   Elisa Izaurralde

   Article

   Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

   Insert

   HseIF4A3 (EIF4A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-6P-1-HsRCK_E

   Plasmid

   #146210

   Purpose

   Bacterial Expression of HsRck

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   HsRck (DDX6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-Akt-Scramble

   Plasmid

   #175014

   Purpose

   Negative-control construct for Akt-STOPS.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   mCherry-Akt-Scramble
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-link-GFPEnvy-SpHis5

    Plasmid

    #60782

    Purpose

    Yeast C-terminal tagging vector, which creates gene fusions with GFPEnvy using SpHis5 selection

    Depositor

    Linda Huang

    Article

    Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

    Insert

    GFPEnvy
    

    Use

    Yeast integrating

    Tags

    Expression

    Mutation

    S30R Y39N T65C S72A F99S N105T Y145F M153T V163A …

    Promoter

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHLP700

    Plasmid

    #203742

    Purpose

    C-terminal tagging of target proteins with AID*/3xHA degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain codons 71-114
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET15b/bPanK

    Plasmid

    #73619

    Purpose

    Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

    Depositor

    Suzanne Jackowski, T. J. Kappock

    Article

    Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

    Insert

    pantothenate kinase (coaA Escherichia coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    relative to MG1655 sequence, changes glutamate-5 …

    Promoter

    T7

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-H2A-Akt-STOPS

    Plasmid

    #175010

    Purpose

    Akt-STOPS targeted to the nucleus via histone H2A.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    H2A-mCherry-Akt-STOPS
    

    Use

    Tags

    Histone H2A and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHD157

    Plasmid

    #84033

    Purpose

    Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafish

    Depositor

    Didier Stainier, Daniel Hesselson

    Article

    Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

    Inserts

    Cre
    Venus
    

    Use

    Cre/Lox; Zebrafish transgenesis

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Crystalin and fabp10

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBK1290-AAV-dSaCas9

    Plasmid

    #223139

    Purpose

    AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-EGFP-C1-HsEDC4_S

    Plasmid

    #147455

    Purpose

    Mammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.

    Depositor

    Elisa Izaurralde

    Article

    Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

    Insert

    HsEDC4 (EDC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p5078 pcDNA4c hBrd4 (1-470)

    Plasmid

    #14454

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 (1-470) (BRD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    amino acids 1-470

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

18. Hox D4a

    Plasmid

    #36469

    Depositor

    Robert Ho

    Article

    Prince et al Development. 1998 Feb . 125(3):407-20.

    Insert

    Hox D4a (hoxd4a Zebrafish)
    

    Use

    Riboprobe synthesis

    Tags

    Expression

    Bacterial

    Mutation

    Q100H, A101T, G125C and R189W

    Promoter

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-AktAR2-NES

    Plasmid

    #175011

    Purpose

    Cytosol-targeted AktAR2.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    AktAR-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTol1:uas-myc-act-map2k2b-2a-tdTomato

    Plasmid

    #104721

    Purpose

    UAS-driven expression of activated MEK

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    activated map2k2b (map2k2b Zebrafish)
    

    Use

    Zebrafish transposon

    Tags

    2A-tdTomato and myc tag

    Expression

    Mutation

    serines 221 and 225 to aspartic acid

    Promoter

    10xUAS

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFlag-CMV2-Brd4 del ET

    Plasmid

    #21938

    Depositor

    Eric Verdin

    Article

    Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1-1362 only, ET domain deleted

    Promoter

    Available since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

22. pSB3K3 containing mRFP1

    Plasmid

    #118083

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, mRFP1
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKV014

    Plasmid

    #64765

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, 3xHA to 3xT7-mRFP tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSB1K3 containing mRFP1

    Plasmid

    #118082

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, mRFP1
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-AktAR2-NLS

    Plasmid

    #175012

    Purpose

    Nuclear-targeted AktAR2.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    AktAR-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Tmem214 pmRFP-N2 (1348)

    Plasmid

    #62047

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem214 (TMEM214 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3-Lyn-Akt-STOPS

    Plasmid

    #175009

    Purpose

    Plasma membrane-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    Lyn-mCherry-Akt-STOPS
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJM195A

    Plasmid

    #218125

    Purpose

    Hybrid circuit displaying hsCRBN-CTD bait and driving expression of gIII

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    CRBN_CTD
    M13 pIII
    

    Use

    Tags

    434cI_RR69

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJM194A

    Plasmid

    #218124

    Purpose

    Hybrid circuit displaying hsCRBN-CTD bait and driving expression of luxAB

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    CRBN_CTD
    luxAB
    

    Use

    Tags

    434cI_RR69

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHL 1075

    Plasmid

    #53004

    Purpose

    pCon:fhlA::mCherry, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    fhlA
    sodB
    

    Use

    Synthetic Biology

    Tags

    GFP and mCherry

    Expression

    Bacterial

    Mutation

    contains −309 to +96 bp relative to start codon a…

    Promoter

    pCon and pLlacO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

31. pKV015

    Plasmid

    #64766

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA-GFP to a T7-RFP tag, invariable V5 tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1-MS2-HA-HsSmg7_T

    Plasmid

    #147556

    Purpose

    Mammalian Expression of HsSmg7

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg7 (SMG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutation G216A, A1521G and one non sil…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. asPink chromoprotein promoter-less

    Plasmid

    #118005

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, asPink
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pT7-EGFP-C1-HsXRN1_1-1173_K

    Plasmid

    #146785

    Purpose

    Mammalian Expression of HsXRN1_1-1173

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    HsXRN1_1-1173 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pT7-EGFP-C1-HsMago_I

    Plasmid

    #146582

    Purpose

    Mammalian Expression of HsMago

    Depositor

    Elisa Izaurralde

    Article

    Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

    Insert

    HsMago (MAGOH Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation Y123C compared to the seq…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pShuttle

    Plasmid

    #192871

    Purpose

    Gateway compatible vector with BsaI cloning site

    Depositor

    Thorsten Stiewe

    Article

    Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

    Type

    Empty backbone

    Use

    Gateway-compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. p5077 pcDNA4c hBrd4 (1-594)

    Plasmid

    #14453

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 (1-594) (BRD4 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    amino acids 1-594

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Flag-TRIM24-C840W

    Plasmid

    #28136

    Depositor

    Michelle Barton

    Article

    Tsai et al Nature. 2010 Dec 16. 468(7326):927-32.

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    A489V and C840W

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pT7-EGFP-C1-HsNot10_K

    Plasmid

    #146775

    Purpose

    Mammalian Expression of HsNot10

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsNot10 (CNOT10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y

    Plasmid

    #148087

    Purpose

    Insect Expression of DmPAN3-W707AG711E-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN3-W707AG711E-dsRNAres (CG11486 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge