We narrowed to 7,223 results for: ALP

1. pDONR223-ZAK

   Plasmid

   #23709

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ZAK (MAP3K20 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pVRC8400-SARS-CoV-2-S2P-B.1.1.529-AVI

   Plasmid

   #181695

   Purpose

   Expresses SARS-CoV-2 S2P protein from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-B.1.1.529 (S )
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   A67V, ∆H69, ∆V70, T95I, G142D, ∆V143, ∆Y144, ∆Y14…

   Promoter

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GCN5 FL-dCas9-CBP core

   Plasmid

   #179556

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GCN5 core-dCas9-CBP core

   Plasmid

   #179555

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pVRC8400-SARS-CoV-2-S2P-XBB-AVI

   Plasmid

   #196622

   Purpose

   Expresses SARS-CoV-2 S2P protein from the XBB variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-XBB (S )
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAdeasy-CMV-AE-EF1α-R2

   Plasmid

   #194928

   Purpose

   expressing two fusion proteins of Ag85B-ESAT6 and Rv2031c-Rv2626c from multi-stage antigens of Mycobacterium tuberculosis

   Depositor

   Limei Wang

   Article

   Wang et al Vaccines (Basel). 2024 Sep 6;12(9):1022.

   Inserts

   AE
   EEF1A1 (EEF1A1 Human)
   R2
   

   Use

   Adenoviral

   Tags

   Ag85B-ESAT6, EF1a, and Rv2031c-Rv2626c

   Expression

   Mutation

   a 48 bp linker (Gly3ser)4 was added between the t…

   Promoter

   CMV and EF1a

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pVRC8400-SARS-CoV-2-S2P-BQ.1.1-AVI

   Plasmid

   #196621

   Purpose

   Expresses SARS-CoV-2 S2P protein from the BQ.1.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-BQ.1.1 (S )
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T19I, L24-, P25-, P26-, A27S, H69-, V70-, G142D, …

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pVRC8400-SARS-CoV-2-S2P-BJ.1-AVI

   Plasmid

   #196620

   Purpose

   Expresses SARS-CoV-2 S2P protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-BJ.1 (S )
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. IL2-AHA740D, E758K-2A-Cherry-RhoAQ63L in pmCherryN1

   Plasmid

   #187288

   Purpose

   Express pmCherry-IL2-AH A740D, E758K-2A-RhoA Q63L

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   AH A740D, E758K, RhoA Q63L

   Promoter

   CMV

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVRC8400-SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D-AVI

    Plasmid

    #176332

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.617 variant containing G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CBP core-dCas9-p300 core

    Plasmid

    #179558

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) & c-terminal fusion of human p300 core (aa 1048-1664) (CREBBP, EP300 Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GCN5 FL-dCas9-p300 core

    Plasmid

    #179554

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid

    #213030

    Purpose

    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

    Plasmid

    #209082

    Purpose

    NFIB and SOX9 mediated astrocye induction

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid

    #213031

    Purpose

    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

    Depositor

    Donna Romero, Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    

    Use

    Luciferase

    Tags

    Firefly luciferase antisense intron (3' UTR)

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE bidirectional tet-on

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid

    #213026

    Purpose

    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

    Plasmid

    #209079

    Purpose

    Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    sfGFP
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

    Plasmid

    #213025

    Purpose

    Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (1-1275)
    

    Use

    Tags

    3C-3xFlag (ORF2p)

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

    Plasmid

    #209086

    Purpose

    Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    TRE4G and hSyn

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVRC8400-SARS-CoV-2-S2P-XBB.1.5-AVI

    Plasmid

    #198465

    Purpose

    Expresses SARS-CoV-2 S2P protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-XBB.1.5 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE

    Plasmid

    #172908

    Purpose

    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Has Service

    AAV1

    Insert

    Ace2N-4AA-mNeon-ST A122D
    

    Use

    AAV and Cre/Lox

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    hSynapsin1

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRK5-mEGFP-RUNX1-MUT

    Plasmid

    #194575

    Purpose

    Mammalian Expression of mEGFP-RUNX1 Mutant (G363Afs*229) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    G363Afs*229

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

    Plasmid

    #209085

    Purpose

    Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    TRE4G and hSyn

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213027

    Purpose

    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    D702Y (RT- reverse transcriptase catalytic dead)

    Promoter

    CMV

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBiT3.1-C_PPP2R2A:1-447

    Plasmid

    #211321

    Purpose

    Mammalian expression of full-length human PPP2R2A. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    PPP2R2A:M1-N447 (PPP2R2A Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBiT3.1-N_PPP2R2A:1-447

    Plasmid

    #211247

    Purpose

    Mammalian expression of full-length human PPP2R2A. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    PPP2R2A:M1-N447 (PPP2R2A Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

    Plasmid

    #209080

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    A53T

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

    Plasmid

    #209078

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    3K mutant: E35K, E46K, E61K

    Promoter

    TRE4G

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)

    Plasmid

    #209081

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    A53T, NAC deletion (aa60-95)

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPMS-2272

    Plasmid

    #232292

    Purpose

    Protein Expression

    Depositor

    Jeremy Nathans

    Insert

    Alpha bungarotoxin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Quas:GFPpA;Betacrystallin:CFP

    Plasmid

    #81181

    Purpose

    Replaces #61376. QUAS driving GFP with beta crystalline CFP eye marker

    Depositor

    Marnie Halpern

    Insert

    QUAS:GFP;beta crys:CFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LSS-SGFP2-aTub

    Plasmid

    #112936

    Purpose

    Visualization of microtubules

    Depositor

    Dorus Gadella

    Insert

    alpha tubulin
    

    Use

    Tags

    LSS-SGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-EGFP-SnxA

    Plasmid

    #232460

    Purpose

    Lentiviral EGFP lipid sensor for PI(3,5)P2

    Depositor

    Ken-Ichi Takemaru

    Insert

    EGFP-SnxA
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HA-Clover

    Plasmid

    #163366

    Purpose

    Backbone for generating N-terminal or C-terminal clover constructs

    Depositor

    Violaine Sée

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDH-EF1-N-cG-Blast

    Plasmid

    #124981

    Purpose

    Express Nanoluc, copGFP

    Depositor

    Kazuhiro Oka

    Insert

    Nanoluc, copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDH-EF1-N-cG-Hph

    Plasmid

    #124980

    Purpose

    Express Nanoluc, copGFP

    Depositor

    Kazuhiro Oka

    Insert

    Nanoluc, copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDH-EF1-N-cG-Zeo

    Plasmid

    #124979

    Purpose

    Express Nanoluc, copGFP

    Depositor

    Kazuhiro Oka

    Insert

    Nanoluc, copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMJA252

    Plasmid

    #128541

    Purpose

    Acceptor vector for alpha and beta TCR

    Depositor

    Marcos Alcocer

    Insert

    truncated human TCR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    bidirectional CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. HP0421_(7-383)_pET21a

    Plasmid

    #29790

    Depositor

    SeWon Suh

    Insert

    hp0421 (HP0421 H. pylori)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-6 and 384-389

    Promoter

    Available since

    March 27, 2012

    Availability

    Academic Institutions and Nonprofits only

42. pCDH-EF1-Luc2-cG-BSD

    Plasmid

    #124982

    Purpose

    Express Luc2, copGFP

    Depositor

    Kazuhiro Oka

    Insert

    Luc2, copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCDH-EF1-N-cG-Neo

    Plasmid

    #124978

    Purpose

    Express Nanoluc, copGFP

    Depositor

    Kazuhiro Oka

    Insert

    Nanoluc, copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. EYFP-Cx43-7

    Plasmid

    #56587

    Depositor

    Michael Davidson

    Insert

    Cx43
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. Halo_aTubulin-pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221901

    Purpose

    Expresses Halo7 fused to alpha tubulin

    Depositor

    Janelia Integrative Imaging

    Insert

    Halo7-aTubulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pENN.AAV.EF1a.eGFP.WPRE.rBG

    Plasmid

    #105547

    Purpose

    AAV expression of EGFP from EF1a promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV8

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3.1-CnA-H151A

    Plasmid

    #191956

    Purpose

    Expression of phosphatase dead mutant of calcineurin A (CnA-H151A).

    Depositor

    Luca Scorrano

    Insert

    Calcineurin subunit alpha H151A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H151A

    Promoter

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET28b(+)_S. cerevisiae Hbs1(1-611)

    Plasmid

    #39336

    Depositor

    SeWon Suh

    Insert

    Elongation factor 1 alpha-like protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

49. AV0.1-BWS-copGFP

    Plasmid

    #124967

    Purpose

    Express copGFP under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    copGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221899

    Purpose

    Expresses alpha tubulin fused to mStayGold

    Depositor

    Janelia Integrative Imaging

    Insert

    mStayGold-aTubulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge