We narrowed to 2,366 results for: clu

1. PB-EEA-g1-PGK-Puro

   Plasmid

   #102908

   Purpose

   PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-guide1-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS2356_Tier3(PB)-PuroR

   Plasmid

   #169655

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRPBSA

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21c-IF3mt

   Plasmid

   #31078

   Depositor

   Linda Spremulli

   Article

   Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

   Insert

   Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes coding sequence for the mature form. Ami…

   Promoter

   Available since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA-NES-ZapCmR2

   Plasmid

   #59015

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
   

   Use

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pPEE17

   Plasmid

   #128361

   Purpose

   Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.

   Depositor

   James Fraser

   Article

   Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

   Insert

   Acetamidase (amdS)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   TEF1

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET14b_tev_PSGU

   Plasmid

   #59738

   Purpose

   Expresses a.a. 417-887 of human ZO-1

   Depositor

   Alan Fanning

   Article

   Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

   Insert

   zonula occludens-1 (TJP1 Human)
   

   Use

   Tags

   HIS tag and thrombin clvg

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

7. PA-RL-mCherry

   Plasmid

   #78861

   Purpose

   DULIP negative control (bait) to exclude unspecific background binding

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   mCherry
   

   Use

   Expression vector

   Tags

   Protein A, Renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDH3

   Plasmid

   #83789

   Purpose

   Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   CFP
   

   Use

   Tags

   CFP

   Expression

   Yeast

   Mutation

   F64L, Y66W, N146I, M153T, V163A, N164H mutations …

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLLX13

   Plasmid

   #79825

   Purpose

   Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

   Depositor

   Cammie Lesser, Stephen Lory

   Article

   Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

   Type

   Empty backbone

   Use

   Yeast and bacteria shuttle vector, contains origi…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dppa1-T2A-H2B-Halo donor

    Plasmid

    #113118

    Purpose

    Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Dppa1 donor region including homology arms and T2A-H2B-Halo
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-NES-ZapCmR1.1

    Plasmid

    #59014

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pCXLE-dCas9GFP-shP53

    Plasmid

    #102906

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9GFP-shP53
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CD44I-c001

    Plasmid

    #175448

    Purpose

    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLY134

    Plasmid

    #130962

    Purpose

    Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson promoter J23100

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. psecBFP2

    Plasmid

    #62230

    Purpose

    encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.

    Depositor

    Erik Snapp

    Article

    Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;

    Insert

    secBFP2
    

    Use

    Tags

    secbfp2

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. UAS::PTPRDb-DN-GFP

    Plasmid

    #37112

    Depositor

    Alvaro Sagasti

    Article

    Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

    Insert

    UAS::PTPRDb-DN-GFP (ptprdb Zebrafish)
    

    Use

    Tol2 gateway destination vector

    Tags

    EGFP

    Expression

    Mutation

    deleted cytodomain, including phosphatase domains…

    Promoter

    UAS

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJLI-SUP35 (delta)2-5

    Plasmid

    #1138

    Depositor

    Susan Lindquist

    Article

    Liu et al Nature 1999 Aug 5;400(6744):573-6.

    Insert

    sup35 delta 2-5 mutant, 5' and 3' flanking regions (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    last four repeats of sup35 N domain deleted, but …

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJUMP18-cat_TU

    Plasmid

    #127051

    Purpose

    Basic Part Whole TU; Codes for chloramphenicol resistance; including promoter and terminator. Broad host range. From Bacillus/E.coli shuttle pSEVA3b61.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part Cat_TU
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLSLZ

    Plasmid

    #51495

    Purpose

    pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    Zif268:FokI
    3kb filler sequence
    

    Use

    Plant t-dna plasmid

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. PHMcc(delta16 PHM 356s)

    Plasmid

    #25455

    Depositor

    Betty Eipper

    Article

    Kolhekar et al Biochemistry. 1997 Sep 9. 36(36):10901-9

    Insert

    PHM (Pam Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the catalytic core of PHM, including aa …

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only