We narrowed to 5,820 results for: pcas

1. pCAGGS-FLAG-BMAL1-S516A

   Plasmid

   #186833

   Purpose

   Expresses FLAG-BMAL1-S516A in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 516 to Alanine

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-FLAG-BMAL1-S519A

   Plasmid

   #186834

   Purpose

   Expresses FLAG-BMAL1-S519A in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 519 to Alanine

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-Flag-S1pr1-R120A

   Plasmid

   #203707

   Purpose

   Overexpression of FLAG-S1PR1-R120A mutant.

   Depositor

   Timothy Hla

   Insert

   Flag-S1PR1-R120A (S1PR1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R120A

   Available Since

   Dec. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA-T-intron(Neo)-G(LNL)

   Plasmid

   #36908

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   T (i.e., ATG, start codon as a first exon)
   GFP
   beta-globin intron
   Neo
   

   Expression

   Mammalian

   Mutation

   deleted nucleotides before nucleotide 275 and ins…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available Since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-GBP-dCas9-mRFP

   Plasmid

   #102933

   Purpose

   Expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

   Depositor

   Sebastian Bultmann

   Article

   Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   GBP (GFP binding protein) and mRFP

   Expression

   Mammalian

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pCAGGS-pac-T2A-iCre

   Plasmid

   #113849

   Purpose

   Puromycin resistance gene and iCre recombinase

   Depositor

   Mario Capecchi

   Insert

   pac-T2A-iCre
   

   Expression

   Mammalian

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-Flag-eSpCas9-D10A

   Plasmid

   #80449

   Purpose

   enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   ehSpCas9_D10A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCASP-SptP167-3G124-HilA

   Plasmid

   #153322

   Purpose

   Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP167-3G124 DARPin
   

   Use

   Salmonella expression

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCASP-HilA empty vector

   Plasmid

   #153333

   Purpose

   Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Type

   Empty backbone

   Use

   Salmonella expression

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMS24: pCascade(PmcCAST)_entry

    Plasmid

    #168157

    Purpose

    Inducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST minimal CRISPR array
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

    Plasmid

    #107305

    Purpose

    Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2x NLS and FKBP

    Expression

    Mammalian

    Mutation

    R1335K and fused to FKBP

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCASP-SptP120-3m160-HilA

    Plasmid

    #153325

    Purpose

    Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-3m160 DARPin
    

    Use

    Salmonella expression

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Trex2-bpA-EF1BFP

    Plasmid

    #111144

    Purpose

    Expression of mouse Trex2 and BFP reporter in mammalian cells

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Inserts

    mouse Trex2 coding region (Trex2 Mouse)
    BFP (Rnf112 )
    

    Expression

    Mammalian

    Promoter

    CAG and EF1

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCasper-pNos-PCP-eGFP

    Plasmid

    #180274

    Purpose

    to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

    Depositor

    Hernan Garcia

    Article

    Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

    Insert

    PCP-eGFP
    

    Tags

    eGFP

    Expression

    Insect

    Promoter

    nanos

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-Flag-eSpCas9-H840A

    Plasmid

    #80454

    Purpose

    enhanced SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    ehSpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9-Puro HLAG-2B-sgRNA

    Plasmid

    #182552

    Purpose

    Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

    Depositor

    Santiago Miriuka

    Article

    Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

    Insert

    hSpCas9-2A-Puro-HLAG-2B-sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCa4B2G-QUAS-DSCP-FLPo

    Plasmid

    #24359

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    QUAS-DSCP-FLPo
    

    Expression

    Insect

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. Osa-pCAMBIA-1301-STTM159

    Plasmid

    #84195

    Purpose

    Disturb the expression of miR159 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR159
    

    Expression

    Bacterial, Plant, and Yeast…

    Promoter

    2x35S

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

    Plasmid

    #148855

    Purpose

    Mammalian Expression of HsAGO3-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO3-sgRNA (AGO3 S.pyogenes)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-SRGAP2A-FBAR-mRFP

    Plasmid

    #168502

    Purpose

    Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

    Depositor

    Franck Polleux

    Article

    Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

    Insert

    SRGAP2A-FBAR (SRGAP2 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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