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Plasmid#212611PurposeFor expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.DepositorInsertTornado-CVB3-nLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDawn
Plasmid#43796PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 sgATG7
Plasmid#211534PurposeDeletes ATG7DepositorInsertsgATG7
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-CDK4KTR
Plasmid#126680PurposeFluorescent reporter for CDK4/6 activityDepositorInsertRB cterm
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pREDawn-AmpR-MCS
Plasmid#188978PurposeFor red light-induced bacterial expression of gene of interest (AmpR)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSFVCs-lacZ
Plasmid#92076PurposeThis is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.DepositorInsertC-lacZ
UseC-lacz expression plasmid, for run-off transcript…TagsExpressionMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
HyperNova/pRSETB
Plasmid#221456PurposeFast-maturating photosensitizing fluorescent protein for chromophore-assisted light inactivationDepositorInsertHyperNova
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MscL(G22S)-c-term-cpGFP-ERexp
Plasmid#195346PurposeControl plasmid for the MscL tension biosensorDepositorInsertMscL(G22S)-c-term-cpGFP-ERexp
UseLentiviralTagsExpressionMammalianMutationPromoterCMVd2Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIGII-TCR-E
Plasmid#194391PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACλCI-β-flap (831-1057)
Plasmid#53733PurposeExpression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertλCI-β-flap fusion
UseTagsExpressionBacterialMutationPromoterlac UV5Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL1-GFP
Plasmid#180420PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-CaM-V5-sTb(N)
Plasmid#219783PurposesTb(N) half of CaST for neuron expressionDepositorInsertCaM-V5-sTb(N)
UseTagsV5ExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265_OoA3GX2
Plasmid#225147PurposeGateway entry clone (attL1 & attR5) OoA3GX2-CBE_V01 for C-T base editingDepositorInsertOoA3GX2-zCas9(D10A)-UGI
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CaM-V5-sTb(N)
Plasmid#219779PurposesTb(N) half of CaST for HEK cell expressionDepositorInsertCaM-V5-sTb(N)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pD441_WT_hi
Plasmid#128857PurposeHigh copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FKBP-mCherry-MCS
Plasmid#178863PurposeMammalian expression of a protein of interest fused to the C-terminus of FKBP-mCherryDepositorInsertFKBP-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFN18A-HaloTag-SpyCatcher
Plasmid#206041PurposeHaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTagDepositorTypeEmpty backboneUseTagsHaloTag and SpyCatcher-8HisExpressionBacterialMutationPromoterT7Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDusk
Plasmid#43795PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -