1,878 results

1. pET_MBP-RGG-RGG

   Plasmid

   #124946

   Purpose

   Expression of MBP-RGG-RGG

   Depositor

   Daniel Hammer, Matthew Good, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   MBP-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Tags

   His and MBP

   Expression

   Bacterial

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJW1659

   Plasmid

   #154314

   Purpose

   NT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::AID*::3xFLAG for NT slot
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BiP 2: C. elegans BiP (hsp-4)-GFP

   Plasmid

   #21896

   Depositor

   David Ron

   Article

   Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

   Insert

   BiP (hsp-4 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Mutation

   Fusion between the GFP and hsp-4 takes place at A…

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDD284

   Plasmid

   #66825

   Purpose

   TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   TagRFP-T-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized TagRFP-T

   Expression

   Worm

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2

   Plasmid

   #182357

   Purpose

   Positive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   GFP
   

   Expression

   Worm

   Promoter

   Peft-3

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159845

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJW1821

   Plasmid

   #163092

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   mScarlet^SEC (Lox511I)^::3xMyc
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJJR82

   Plasmid

   #75027

   Purpose

   EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Article

   Ramalho et al Development. 2020 Jul 22;147(14):dev1880

   Insert

   EGFP^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized EGFP

   Expression

   Worm

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFJ352 - MCS(ttTi4348, I)

   Plasmid

   #34868

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   ttTi4348 targeting
   

   Use

   Worm targeting

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJJR83

    Plasmid

    #75028

    Purpose

    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Article

    Ramalho et al Development. 2020 Jul 22;147(14):dev1880

    Insert

    mCherry^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized mCherry

    Expression

    Worm

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBN1

    Plasmid

    #86710

    Purpose

    C. elegans injection marker

    Depositor

    Peter Askjaer

    Article

    Rodenas et al Mol Biol Cell. 2012 Mar;23(5):930-44. do

    Insert

    Plmn-1::mCherry::his-58::pie-1 3'UTR
    

    Expression

    Worm

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mg319

    Plasmid

    #73367

    Purpose

    unc-70 tension sensor

    Depositor

    Miriam Goodman

    Article

    Krieg et al Nat Cell Biol. 2014 Mar;16(3):224-33. do

    Insert

    unc-70::TSMod
    

    Promoter

    unc-70p

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJW1816

    Plasmid

    #154320

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSEM237 - pEXP[Prpl-3 | histamine | rpl-3 UTR]

    Plasmid

    #159796

    Purpose

    Histamine based negative selection marker to paralyze animals with arrays

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

    Insert

    pEXP[Prpl-3 | histamine | rpl-3 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TU#867 (Punc-119sid-1)

    Plasmid

    #25811

    Depositor

    Martin Chalfie

    Article

    Calixto et al Nat Methods. 2010 Jul;7(7):554-9. doi: 1

    Insert

    systemic RNA interference-deficient 1 (sid-1 Nematode)
    

    Expression

    Worm

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pCFJ1415

    Plasmid

    #84828

    Purpose

    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 introns

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    C. elegans codon optimized GFP
    

    Expression

    Bacterial and Worm

    Promoter

    Peft-3

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET_RGG

    Plasmid

    #124929

    Purpose

    Expression of RGG domain from LAF-1 gene

    Depositor

    Daniel Hammer, Matthew Good, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    LAF-1 RGG domain (laf-1 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    RGG domain

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDD315 (mTurquoise2^SEC^2xHA)

    Plasmid

    #73343

    Purpose

    mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

    Insert

    mTurquoise2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    2xHA and C. elegans codon-optimized mTurquoise2

    Expression

    Worm

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXF121

    Plasmid

    #139028

    Purpose

    MosSCI SapTrap assembly: Cassette 1 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    short mex-5 promoter (mex-5 Nematode)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus

    Plasmid

    #182344

    Purpose

    sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Spacer 4
    

    Expression

    Worm

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge