1,862 results

1. pXF89

   Plasmid

   #139030

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   halotag (no STOP codon, PATC introns)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. KG#908

   Plasmid

   #110914

   Purpose

   Expresses the plant proteins TIR1 (necessary for Auxin-Inducible Degradation) pan-neuronally in C. elegans

   Depositor

   Kenneth Miller

   Inserts

   rab-3 promoter
   TIR1 cDNA
   unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEY139

   Plasmid

   #223486

   Purpose

   tph-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   tph-1 (tph-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSX2719

   Plasmid

   #200763

   Purpose

   Expresses mCherry fused MS2 coat protein and 24xGCN4 (Suntag) with the tissue-specific promoter semo-1 in C. elegans.

   Depositor

   Suhong Xu

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   MCP-24xGCN4
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   semo-1

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSX2720

   Plasmid

   #200764

   Purpose

   Expresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans.

   Depositor

   Suhong Xu

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   scFv-sfGFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   col-19

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEY82

   Plasmid

   #191077

   Purpose

   flp-7 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   flp-7 (flp-7 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCZGY847

   Plasmid

   #135093

   Purpose

   Transcriptional mcherry reporter of acr-2 (cholinergic) expressing neurons in C. elegans

   Depositor

   Yishi Jin

   Article

   Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

   Insert

   Pacr-2
   

   Use

   Tags

   mcherry and unc-10 3'UTR

   Expression

   Worm

   Mutation

   Promoter

   Pacr-2

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSEM272 - MCS mosTI Pmlc-2_GFP target

   Plasmid

   #159834

   Purpose

   Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   MCS mosTI Pmlc-2_GFP target
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr

   Plasmid

   #19326

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Pmyo-3::twk-18(cn110)cDNA
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK2014

    Plasmid

    #171931

    Purpose

    To express sygl-1 fused to 24 copies of MS2 loops.

    Depositor

    Judith Kimble

    Article

    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

    Insert

    Psygl-1::24XMS2 loops::3X Flag::sygl-1 ORF::sygl-1 3'UTR
    

    Use

    Tags

    N-terminal Tag 24X MS2 loops

    Expression

    Worm

    Mutation

    Promoter

    -

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. JH02

    Plasmid

    #73511

    Purpose

    mCherryo_PH pCFJ150 - contians C. elegans sequence optimized mCherry

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::mCherryo::PH::tbb-2
    

    Use

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    pmex-5

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. punc-119c

    Plasmid

    #21595

    Depositor

    Al Fisher

    Article

    Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

    Insert

    unc-119 (unc-119 Nematode)
    

    Use

    Adds unc-119 marker to amp resistant plasmids

    Tags

    Expression

    Worm

    Mutation

    N/A

    Promoter

    Available since

    Aug. 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJR019

    Plasmid

    #198834

    Purpose

    Inactivation of G protein subunits through Pertussis toxin (PTX)

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::PTX-SL2-GFP::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCZGY546

    Plasmid

    #90466

    Purpose

    Pmec-4::mcherry, Expresses mcherry red fluorescence in C. elegans touch neurons

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pmec-4-mcherry
    

    Use

    Tags

    mcherry

    Expression

    Worm

    Mutation

    Promoter

    Pmec-4

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJL44 - Phsp16.48::MosTase::glh-2utr

    Plasmid

    #19333

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Phsp-16::Mos1 transposase
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJW2086

    Plasmid

    #154335

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP::AID*::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDD285

    Plasmid

    #66826

    Purpose

    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mKate2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized mKate2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

18. KG#62

    Plasmid

    #110874

    Purpose

    Expresses the C. elegans acy-1 cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

    Inserts

    rab-3 promoter
    acy-1 cDNA
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJR022

    Plasmid

    #198820

    Purpose

    C. elegans optimized version of mScarlet

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::wrmScarlet::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCZGY2850

    Plasmid

    #87758

    Purpose

    Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pcol-19-PH-miniSOG(Q103L)
    

    Use

    Tags

    PH

    Expression

    Worm

    Mutation

    Q103L

    Promoter

    Pcol-19

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge