We narrowed to 259 results for: apex2

1. pAAV-EF1a-DIO-LckAPEX-P2A-EGFP

   Plasmid

   #182826

   Purpose

   Cre-dependent expression of membrane localized APEX

   Depositor

   Yevgenia Kozorovitskiy

   Article

   Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

   Insert

   LckAPEX-P2A-EGFP
   

   Use

   AAV

   Tags

   EGFP reporter (P2A)

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS614_pTetR-P2A-BFPnls/sgNS

   Plasmid

   #108650

   Purpose

   Non-specific Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Non-specific sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS613_pTetR-P2A-BFPnls/sgTelo

   Plasmid

   #108649

   Purpose

   Telomere-targeting Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Telomere-targeting sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SOPP3-HA-nes

   Plasmid

   #229071

   Purpose

   Expresses SOPP3 in the cytosol of mammalian cells

   Depositor

   Weijun Pan

   Article

   Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

   Insert

   Express SOPP3 in the cytosol of mammalian cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTXB1-TP5

   Plasmid

   #160088

   Purpose

   Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA

   Depositor

   Pier Pandolfi

   Article

   Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

   Insert

   Tn5 transposase/APEX2 peroxidase fusion protein
   

   Use

   Tags

   FLAG and Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTXB1-TP3

   Plasmid

   #160086

   Purpose

   Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA

   Depositor

   Pier Pandolfi

   Article

   Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

   Insert

   Tn5 transposase/APEX2 peroxidase fusion protein
   

   Use

   Tags

   FLAG and Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS887_pTetR-P2A-BFPnls/sgAlpha

   Plasmid

   #108651

   Purpose

   Alpha satellite repeats targeting Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Alpha satellite repeats targeting sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTXB1-TP4

   Plasmid

   #160087

   Purpose

   Bacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4

   Depositor

   Pier Pandolfi

   Article

   Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

   Insert

   Tn5 transposase/APEX2 peroxidase fusion protein
   

   Use

   Tags

   FLAG and Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTXB1-TP1

   Plasmid

   #160084

   Purpose

   Bacterial expression of Tn5-APEX2 fusion protein, no linker

   Depositor

   Pier Pandolfi

   Article

   Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

   Insert

   Tn5 transposase/APEX2 peroxidase fusion protein
   

   Use

   Tags

   FLAG and Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTXB1-TP2

    Plasmid

    #160085

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Use

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid

    #167288

    Purpose

    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    APEX2 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Inducible Caspex expression

    Plasmid

    #97421

    Purpose

    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

    Depositor

    Steven Carr, Samuel Myers

    Article

    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

    Inserts

    Caspex
    T2A-Gfp
    

    Use

    CRISPR

    Tags

    3xFLAG and V5

    Expression

    Mammalian

    Mutation

    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

    Promoter

    TRE3G

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2 LRP6-eGFP

    Plasmid

    #180143

    Purpose

    to make movies of LRP6 vesicles

    Depositor

    Edward De Robertis

    Article

    Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

    Insert

    Human LRP6 (LRP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2-LRP6-3XFlag

    Plasmid

    #180144

    Purpose

    flag tagged LRP6

    Depositor

    Edward De Robertis

    Article

    Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

    Insert

    Human LRP6 (LRP6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-AP-SpyCatcher

    Plasmid

    #223636

    Purpose

    Protein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His10, AP fragment of split APEX2 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS L30 Flag-Apex-Rab7A

    Plasmid

    #135652

    Purpose

    Rab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Tags

    Apex2 and FLag

    Expression

    Mammalian

    Mutation

    Promoter

    L30

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pML433

    Plasmid

    #177190

    Purpose

    Expresses recombinant HyPro enzyme in E. coli

    Depositor

    Eugene Makeyev

    Article

    Yap et al Mol Cell. 2021 Nov 2. pii: S1097-2765(21

    Insert

    HyPro enzyme
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 tdAPX W41F

    Plasmid

    #40863

    Purpose

    Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

    Insert

    tandem dimer pea APX W41F
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    W41F mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57 APEX

    Plasmid

    #40306

    Purpose

    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

    Insert

    pea APEX (Synthetic)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    K14D, W41F, E112K (relative to wild-type)

    Promoter

    Available since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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