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Showing: 321 - 360 of 548 results

  11. pTetON-DHFRdd-SpvB; CMV-mCherry

    Plasmid
    #89463
    Purpose
    Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)
    Depositor
    Brad Zuchero
    Article
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Insert
    DeAct-SpvB (spvB Salmonella enterica)
    Use
    Tags
    E. coli DHFRdd destabilization domain and mCherry…
    Expression
    Mammalian
    Mutation
    Truncation spanning SpvB amino acids 375-591 (mon…
    Promoter
    TRE3G
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    89463-plasmid-map-sequence-id-176808

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  13. pDONR221-SLC11A2_STOP

    Plasmid
    #161050
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC11A2 (SLC11A2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161050-plasmid-map-sequence-id-362755

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  14. pThermoCas9i_ctrl

    Plasmid
    #100982
    Purpose
    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    changed aspartate 8 to alanine and histidine 582 …
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100982-plasmid-map-sequence-id-392326

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  15. pET28a_DmKHC[1-421]-1xGFP-6xHis

    Plasmid
    #196972
    Purpose
    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag
    Depositor
    Lukas Kapitein
    Article
    Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    6xHis and GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196972-plasmid-map-sequence-id-388918

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  27. pEXSISERS_SurfaceHaloTag_FRT-PuroR

    Plasmid
    #177869
    Purpose
    Cloning Backbone for Surface-HaloTag-based EXSISERS containing FRT-sites flanked PuroR cassette; clone homology arms via BbsI (or BpiI).
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d
    Insert
    Surface-HaloTag-based EXSISERS
    Use
    CRISPR, Synthetic Biology, and Unspecified
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    177869-plasmid-map-sequence-id-358833

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  28. pCjedCas9

    Plasmid
    #172216
    Purpose
    Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells
    Depositor
    John van der Oost
    Article
    Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi
    Insert
    catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
    Use
    Tags
    Histag
    Expression
    Mutation
    changed Asp 8 to Ala and His 559 to Ala
    Promoter
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    172216-plasmid-map-sequence-id-338133

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  29. pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK

    Plasmid
    #177868
    Purpose
    Cloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d
    Insert
    mGreenLantern-based EXSISERS
    Use
    CRISPR, Synthetic Biology, and Unspecified
    Tags
    OLLAS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    177868-plasmid-map-sequence-id-358838

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Showing: 321 - 360 of 548 results