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Showing: 401 - 450 of 11379 results

  4. pLL3.7-EF-EYFP-YAP1_WW-PolyA

    Plasmid
    #112290
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDA
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Human YAP1-2gamma with mutations in the WW domain…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112290-plasmid-map-sequence-id-213664

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  6. mScarlet-I-T2A-LSS-SGFP2

    Plasmid
    #112946
    Purpose
    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
    Depositor
    Dorus Gadella
    Insert
    mScarlet-I and LSS-SGFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112946-plasmid-map-sequence-id-223584

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  8. LifeAct-LSS-SGFP2-N1

    Plasmid
    #112938
    Purpose
    Visualization of F-actin
    Depositor
    Dorus Gadella
    Insert
    LifeAct
    Use
    Tags
    LSS-SGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112938-plasmid-map-sequence-id-223583

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  13. pOP2HA

    Plasmid
    #112598
    Purpose
    Used for expression of protein in E. coli as non-cleavable C-terminal 8His-Avi tag fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-Avi tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112598-plasmid-map-sequence-id-220206

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  14. CvcE in pGEX4t

    Plasmid
    #112977
    Purpose
    For protein expression and purification of Drosophila CvcE
    Depositor
    Terry Orr-Weaver
    Insert
    cycE (CycE Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    112977-plasmid-map-sequence-id-217475

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  15. pTFE3-donor

    Plasmid
    #112377
    Purpose
    CRISPR donor plasmid to tag human transcription factor TFE3 with GFP
    Depositor
    Kevin White
    Insert
    TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112377-plasmid-map-sequence-id-229742

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  21. pOP1

    Plasmid
    #112595
    Purpose
    Used for expression of protein in E. coli as non-fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112595-plasmid-map-sequence-id-220200

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  23. pGDP3 rmtB

    Plasmid
    #112880
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    rmtB (RmtB Escherichia coli)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112880-plasmid-map-sequence-id-219870

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  25. LUC56 (Luc/UGA/SP)

    Plasmid
    #112147
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Photinus pyralis, Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258mutated to selenocysteine (U)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112147-plasmid-map-sequence-id-214894

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  26. pGDP4 ermC

    Plasmid
    #112884
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    ermC (ermM Staphylococcus epidermis)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Plac
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112884-plasmid-map-sequence-id-219861

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  28. pOP3GT

    Plasmid
    #112607
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and GST fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Avi - 8His-GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112607-plasmid-map-sequence-id-220236

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  29. mScarlet-I-LSS-SGFP2

    Plasmid
    #112945
    Purpose
    Produces a fusion between LSS-GFP2 and mScarlet-I. It can be used as a positive control for FRET
    Depositor
    Dorus Gadella
    Insert
    LSS-GFP2 mScarlet-I Fusion
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112945-plasmid-map-sequence-id-223563

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  30. pOP5BT

    Plasmid
    #112609
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-GB1 and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112609-plasmid-map-sequence-id-220239

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  32. rat APOBEC1 E63A

    Plasmid
    #112861
    Purpose
    plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    APOBEC1 (Apobec1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Glutamic acid 63 to Alanine
    Promoter
    CMV promoter
    Available since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    112861-plasmid-map-sequence-id-296442

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  33. pJUNB-donor

    Plasmid
    #112313
    Purpose
    CRISPR donor plasmid to tag human transcription factor JUNB with GFP
    Depositor
    Kevin White
    Insert
    JUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:12792992:C>G, hg38:19:12793018:T>G,…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112313-plasmid-map-sequence-id-228468

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  36. pTfR-mTurquoise2

    Plasmid
    #112953
    Purpose
    In vivo visualization of Transferrin receptors (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Insert
    pTfR
    Use
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    112953-plasmid-map-sequence-id-223490

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  37. pLL3.7-EF-EYFP-YAP1_delta5C-PolyA

    Plasmid
    #112289
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the protein
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Human YAP1-2gamma lacking the last 5 Amino acids …
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112289-plasmid-map-sequence-id-213521

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  38. RhoA sensor (LSS-SGFP2-Scarlet-I)

    Plasmid
    #112947
    Purpose
    RhoA FRET biosensor
    Depositor
    Dorus Gadella
    Insert
    RhoA sensor
    Use
    Tags
    LSS-SGFP2 and mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112947-plasmid-map-sequence-id-220211

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  39. pLL3.7-EF-EYFP-YAP1_Y3F-PolyA

    Plasmid
    #112288
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoform
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    3 Tyrosines (341, 357 and 394 corresponding to ty…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112288-plasmid-map-sequence-id-213531

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  40. pHEY1.1.0-gDNA

    Plasmid
    #112393
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HEY1
    Depositor
    Kevin White
    Insert
    HEY1 (HEY1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112393-plasmid-map-sequence-id-230239

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  41. pHEY1-donor

    Plasmid
    #112297
    Purpose
    CRISPR donor plasmid to tag human transcription factor HEY1 with GFP
    Depositor
    Kevin White
    Insert
    HEY1 homology arms flanking EGFP-IRES-Neo cassette (HEY1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:8:79765311:G>A
    Promoter
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    112297-plasmid-map-sequence-id-225585

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  43. pGDP3 arr

    Plasmid
    #112890
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    arr
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112890-plasmid-map-sequence-id-219900

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  44. pNAS1b split mCherry NEDD4 WW2 pos ctrl

    Plasmid
    #112029
    Purpose
    Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
    Use
    Tags
    Split mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112029-plasmid-map-sequence-id-213508

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  47. pcDNA3.1-hRIPK1-Flag-K377R

    Plasmid
    #112489
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K377R
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112489-plasmid-map-sequence-id-214536

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  48. rd-his

    Plasmid
    #112911
    Purpose
    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag and non-cleavable C-terminal 6-his tag
    Depositor
    C. James McKnight
    Article
    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20
    Insert
    dematin (DMTN Human)
    Use
    Tags
    6-his tag, Glutathione-S-Transferase, and preciss…
    Expression
    Bacterial
    Mutation
    89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAG…
    Promoter
    T7
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112911-plasmid-map-sequence-id-220214

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  49. pSNAI2-donor

    Plasmid
    #112378
    Purpose
    CRISPR donor plasmid to tag human transcription factor SNAI2 with GFP
    Depositor
    Kevin White
    Insert
    SNAI2 homology arms flanking EGFP-IRES-Neo cassette (SNAI2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112378-plasmid-map-sequence-id-229744

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Showing: 401 - 450 of 11379 results