We narrowed to 12,598 results for: 112
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pAAV.CAG-FLEX.iGABASnFR.F102Y.Y137L
Plasmid#112168PurposeGABA SensorDepositorInsertiGABASnFR.F102Y.Y137L
UseAAVMutationF102Y.Y137LPromoterCAGAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FRB-myc-RagA
Plasmid#112740Purposeexpress FRB-myc-RagADepositorAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP5MP
Plasmid#112612PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminual 8His and MBP tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneTags8His-MBP and AviExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-2HMG-eGFP
Plasmid#112737PurposeExpress 2HMG-eGFP in E. coliDepositorInsert2HMG-eGFP
Tags6xHisExpressionBacterialPromoterT7 PromoterAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
ITPKA_3xmTurquoise
Plasmid#112961PurposeIn vivo visualization of F-actin (can be used for colocalization studies)DepositorInsertITPKA
Tags3x mTurquoise2ExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP5GT
Plasmid#112611PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneTags8His-GST and AviExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR-Blunt_hNANOG
Plasmid#112639PurposePlasmid for PCR amplification of hNANOG DNA template for in vitro transcriptionDepositorAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1665
Plasmid#112039PurposeIntegrating plasmid for expression of ER localized mCherry at TRP1 locusDepositorInsertER-mCherry
ExpressionYeastPromoterTPIAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAZ-donor
Plasmid#112359PurposeCRISPR donor plasmid to tag human transcription factor MAZ with GFPDepositorInsertMAZ homology arms flanking EGFP-IRES-Neo cassette (MAZ Human)
UseCRISPRMutationhg38:16:29809765:G>AAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRNATU6.1 HDAC3 shRNA3
Plasmid#112272PurposeHDAC3 KDDepositorAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAT5
Plasmid#112586PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal 6His-tag fusionsDepositorTypeEmpty backboneTags6HisExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag RagB
Plasmid#112755Purposeexpress Flag-RagBDepositorAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-2HMG-mCherry
Plasmid#112739PurposeExpress 2HMG-mCherry in E. coliDepositorInsert2HMG-mCherry
Tags6xHisExpressionBacterialPromoterT7 PromoterAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV Gsk3 sgRNA/GFP
Plasmid#112733PurposeGsk3b targeting gRNA cloned into px552 (SpGuide) plasmid.DepositorInsertGFP
UseAAV and CRISPRExpressionMammalianAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
LPA2R-P2A-mCherry
Plasmid#112932PurposePlasmid co-producing the LPA2 receptor and a mCherryDepositorInsertLPA2 receptor
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pERF.1.0-gDNA
Plasmid#112395PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ERFDepositorAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pERF-donor
Plasmid#112299PurposeCRISPR donor plasmid to tag human transcription factor ERF with GFPDepositorInsertERF homology arms flanking EGFP-IRES-Neo cassette (ERF Human)
UseCRISPRAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP2S
Plasmid#112597PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal Strep Tag fusionsDepositorTypeEmpty backboneTagsStrep tagExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAB11A-BFP HDRT Source (pTR 145)
Plasmid#112014PurposeDNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFPDepositorInsertRAB11A-BFP HDRT (RAB11A Human)
UseCRISPR and Synthetic BiologyAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAT
Plasmid#112582PurposeUsed for expression of protein in E. coli as non-cleavable N-terminal 6His-tag fusionsDepositorTypeEmpty backboneTags6HisExpressionBacterialAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_WW-PolyA
Plasmid#112290PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDADepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with mutations in the WW domain…PromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-APIGH
Plasmid#112804Purposegateway cloning destination vector to express enhancer driven Gal4 in DrosophilaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSopB2xHA
Plasmid#112513PurposePsopB driven sopB-2xHA and sigEDepositorInsertsopB-2xHA/sigE operon
Tags2xHAExpressionBacterialMutationSopB C-terminal 2xHAPromoterPsopBAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FRB-myc-RagC
Plasmid#112741Purposeexpress FRB-myc-RagCDepositorAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExp-DsbA(SS)
Plasmid#112563PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-DsbA(SS) and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
mScarlet-I-T2A-LSS-SGFP2
Plasmid#112946PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmScarlet-I and LSS-SGFP2
ExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1667
Plasmid#112041PurposeCEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URADepositorInsertERG5
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1Available SinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-APIC-LexAGAD
Plasmid#112807Purposegateway cloning destination vector to express enhancer driven LexAGAD in DrosophilaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1660
Plasmid#112034PurposeC-terminal tag with AID*(AtIAA17_71-114)-HA marked with LEUDepositorInsertIAA17
TagsHAExpressionYeastMutationonly amino acids 71-114PromoterNoneAvailable SinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-LSS-SGFP2-N1
Plasmid#112938PurposeVisualization of F-actinDepositorInsertLifeAct
TagsLSS-SGFP2ExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCL6.1.0-gDNA
Plasmid#112485PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor BCL6DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-NM
Plasmid#1127DepositorAvailable SinceMarch 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R49A)
Plasmid#112726PurposeArg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R49A)
Tags6His-TEVExpressionBacterialMutationR49APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH
Plasmid#112212PurposeTargeted DNA methylationDepositorAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOP2HA
Plasmid#112598PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal 8His-Avi tag fusionsDepositorTypeEmpty backboneTags8His-Avi tagExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1
Plasmid#112646PurposeExpression S.cerevisiaeDepositorInsertGcs1
ExpressionYeastPromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)3x
Plasmid#112057PurposeACTB mRNA tagged with 3 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
TagsRiboglow RNA tagExpressionMammalianMutation3 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)2x
Plasmid#112056PurposeACTB mRNA tagged with 2 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
TagsRiboglow RNA tagExpressionMammalianMutation2 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
CvcE in pGEX4t
Plasmid#112977PurposeFor protein expression and purification of Drosophila CvcEDepositorInsertcycE (CycE Fly)
ExpressionBacterialAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box
Plasmid#112257PurposeExpresses RGS component of opto-RGS2 in mammalian cellsDepositorInsertCRY2-mCherry-(GGGGS)3-hRGS2box
UseLentiviralTagsmCherryExpressionMammalianPromoterCMVAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-3xflagGREB1b
Plasmid#112222Purposemammalian expression of flag tagged GREB1bDepositorAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1aMS2p65HSF1hyg-W
Plasmid#112920PurposeLentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoterDepositorInsertMS2p65HSF1-T2Ahyg
UseCRISPR and LentiviralExpressionMammalianPromoterHuman EF1a promoterAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOP3GT
Plasmid#112607PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and GST fusionsDepositorTypeEmpty backboneTagsAvi - 8His-GSTExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP5BT
Plasmid#112609PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneTags8His-GB1 and AviExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/FFT-LL5BIP
Plasmid#112830Purposetransient/stable overexpressionDepositorAvailable SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSopBWT
Plasmid#112512PurposePsopB driven sopB and sigEDepositorInsertsopB/sigE operon
ExpressionBacterialPromoterPsopBAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
rat APOBEC1 E63A
Plasmid#112861Purposeplasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cellsDepositorArticleInsertAPOBEC1 (Apobec1 Rat)
ExpressionMammalianMutationchanged Glutamic acid 63 to AlaninePromoterCMV promoterAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_delta5C-PolyA
Plasmid#112289PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the proteinDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma lacking the last 5 Amino acids …PromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1673
Plasmid#112047PurposeCEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URADepositorInsertSCT1
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only
