We narrowed to 426 results for: AAVS1

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pSLQ11474

Plasmid

#183624

Purpose

PB-STAT6RE-minCMV-BFP-Triplex-crAAVS1_6-SV40pA

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crAAVS1_6)

Use

CRISPR

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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MTK234_032

Plasmid

#123995

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA1 -hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_033

Plasmid

#123996

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA2 -hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_034

Plasmid

#123997

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA3 -hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK7_013

Plasmid

#123880

Purpose

Encodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RA_hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK8b_011

Plasmid

#123898

Purpose

Encodes the 3' 500 bp hAAVS1 locus homology arm as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LA_hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPPC019.AAV

Plasmid

#171147

Purpose

Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

hAAVS1 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 and J1-BBa_J23117

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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MTK0_051

Plasmid

#123948

Purpose

Encodes the hAAVS1 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hAAVS1 landing pad destination vector

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_057

Plasmid

#123950

Purpose

Encodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BxBI LP on hAAVS1 (Hygro::mRuby)

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pPPC018.AAV

Plasmid

#171146

Purpose

Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

hAAVS1 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 and J1-BBa_J23117

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC010.AAV

Plasmid

#171175

Purpose

Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

hAAVS1 scRNA
Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

BBa_J23119 and Sp.pCas9, BBa_J23107

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Donor-attp-u6-blank-sgrna-backbone

Plasmid

#234076

Purpose

Blank donor for Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

Depositor

Gary Hon

Article

Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

Insert

sgRNA-Blank

Use

Lentiviral

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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reporter-gT1

Plasmid

#47320

Purpose

TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

minCMV-dTomato_pA

Use

CRISPR

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only

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reporter-gT2

Plasmid

#47321

Purpose

TF reporter for gRNA-AAVS1_T2 (RNA-guided dTomato expression)

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

minCMV-dTomato_pA

Use

CRISPR

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
LT0

Plasmid

#210551

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT0::mCherrySSM

Expression

Plant

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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LT1

Plasmid

#210552

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT1::mCherrySSM

Expression

Plant

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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LT2

Plasmid

#210553

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT2::mCherrySSM

Expression

Plant

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMVS184

Plasmid

#176294

Purpose

Reporter_ZIFUAS_minCMVpromoter_GFP_AAVS1_Puromycin

Depositor

Max Staller

Article

Staller et al Cell Syst. 2022 Apr 20;13(4):334-345.e5.

Insert

Promoter and GFP

Tags

GFP

Expression

Mammalian

Promoter

4x Zinc finger binding sites and mini CMV promoter

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR-SP-Cas9 reporter

Plasmid

#62733

Purpose

Mammalian CRISPR-SP-Cas9 reporter

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

CRISPR-SP-Cas9 target seq + tdTomato (out of frame)

Use

CRISPR and Lentiviral

Tags

CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

Expression

Mammalian

Promoter

EF1-Alpha

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCLV3Cas-LT0

Plasmid

#210548

Purpose

Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT0::mCherrySSM

Expression

Plant

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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ipYUC2Cas-LT1

Plasmid

#210559

Purpose

Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by M. polymorpha YUC2 promoter.

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pMpEF1::XVE::UBQ10t::LexA::SpCas9::PEA3AT::pU6::AAVS1gRNA::pMpEF1::LT1::Hygromycin

Expression

Plant

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1Cas-LT1

Plasmid

#210556

Purpose

Binary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1p

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin

Expression

Plant

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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hAAVS-RPL23a-SNAP

Plasmid

#242187

Purpose

Express RPL23a-SNAP in mammalian cells

Depositor

Joshua Mendell

Article

Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

Insert

RPL23a (RPL23A Human)

Tags

SNAP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pbG-APRT-J-u32uni-PdTKmCh

Plasmid

#107279

Purpose

APRT c.407T>C (APRT*J) donor vector with unilateral microhomology

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Inserts

APRT (APRT Human)
puro-deltaTK; CAG::mCherry

Mutation

c.402A>T, c.407T>C

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pC13P-iCLHN

Plasmid

#66580

Purpose

CLYBL safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-C13 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Nanoluc-HaloTag

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pDONOR-MARINA

Plasmid

#223328

Purpose

DONOR vector for genomic integration of MARINA voltage sensitive indicator gene from Platisa et al., 2017 (10.1021/acschemneuro.6b00234).

Depositor

Jonathan Arias

Article

Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

Inserts

MARINA
KIR2.1 channel Golgi-to-plasma membrane trafficking signal

Use

CRISPR and Synthetic Biology

Tags

mCherry and super-ecliptic pHluorin

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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