We narrowed to 742 results for: GB1

1. GB1-His-TET1-878-911-L900A(pL91)

   Plasmid

   #124395

   Purpose

   E.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-L900A (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   L900A

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GB1-His-TET1-878-911-T898E(pL91)

   Plasmid

   #124394

   Purpose

   E.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-T898E (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   T898E

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GB1-His-TET1-878-911-T898A(pL91)

   Plasmid

   #124393

   Purpose

   E.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-T898A (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   T898A

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GB1-His-TET1-878-911-L897A(pL91)

   Plasmid

   #124392

   Purpose

   E.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-L897A (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   L897A

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GB1-His-TET1-878-911-I894A(pL91)

   Plasmid

   #124391

   Purpose

   E.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-I894A (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   I894A

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

   Plasmid

   #121966

   Purpose

   Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   superfold GFP-GB1-NLS-iLID

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)

   Plasmid

   #99343

   Purpose

   Bacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TAD

   Depositor

   Peter Wright

   Article

   Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

   Inserts

   Hif1a (776-826) (HIF1A Human)
   CBP (340-439)
   

   Use

   Tags

   His6GB1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)

   Plasmid

   #99342

   Purpose

   Bacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TAD

   Depositor

   Peter Wright

   Article

   Wojciak et al EMBO J. 2009 Apr 8;28(7):948-58. doi: 10

   Inserts

   STAT1 (710-750) (STAT1 Human)
   CBP (1764-1855)
   

   Use

   Tags

   His6GB1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pnEA-NpG-DmNanos-opt_50-236-del116-163-GB1opt_AB

   Plasmid

   #148330

   Purpose

   Bacterial Expression of DmNanos-opt_50-236-del116-163

   Depositor

   Elisa Izaurralde

   Article

   Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

   Insert

   DmNanos-opt_50-236-del116-163 (nos Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pnEA-NpM-DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676-GB1_AD

    Plasmid

    #148512

    Purpose

    Bacterial Expression of DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676 (eIF4G1 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. H6GB1-CITED2(216-269APAA) + CBP TAZ1(340-439)

    Plasmid

    #189963

    Purpose

    Coexpression of CBP TAZ1 and CITED2 in E coli

    Depositor

    Peter Wright

    Article

    Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

    Inserts

    CITED2 (216-269) (CITED2 Human)
    CBP TAZ1 (340-439) (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L243A, E245A, L246A

    Promoter

    t7

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. H6Gb1-CITED2(216-246)+CBP TAZ1(340-439)

    Plasmid

    #189962

    Purpose

    Expression of CBP TAZ1 and CITED2 in E coli

    Depositor

    Peter Wright

    Article

    Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

    Inserts

    CITED2 (216-246) (CITED2 Human)
    CBP TAZ1 (340-439) (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    t7

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GB1-PRD(703-868, P801G, T805C, C813S)-Strep

    Plasmid

    #169749

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, T805C, C813S)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Proline 801 to Glycine, Threonine 805 to Cysteine…

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GB1-PRD(703-868, P801G, G808C, C813S)-Strep

    Plasmid

    #169750

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Proline 801 to Glycine, Glycine 808 to Cysteine, …

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GB1-PRD(703-868, S712C, P801G, C813S)-Strep

    Plasmid

    #164445

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

    Insert

    GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Serine 712 to Cysteine, Proline 801 to Glycine, C…

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GB1-PRD(703-868, A756C, P801G, C813S)-Strep

    Plasmid

    #164446

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

    Insert

    GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Alanine 756 to Cysteine, Proline 801 to Glycine, …

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GB1-PRD(703-868, P801G, C813S, S863C)-Strep

    Plasmid

    #164447

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Proline 801 to Glycine, Cysteine 813 to Serine, S…

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)

    Plasmid

    #78507

    Purpose

    Construct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labeling

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Gads SH3 SH2 SH3 domain (GRAP2 Human)
    

    Use

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. cmv-sfGFP-GB1-scAB-ubc-nls-pcp-10xSunTag(SRv1.1-2P)

    Plasmid

    #185801

    Purpose

    one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.1

    Depositor

    Robin Lee

    Article

    Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

    Insert

    cmv-sfGFP-GB1-scAB--ubc-nls-pcp-10xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P)

    Plasmid

    #185802

    Purpose

    one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2

    Depositor

    Robin Lee

    Article

    Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

    Insert

    cmv-sfGFP-GB1-scAB--ubc-nls-pcp-12xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. cmv-sfGFP-GB1-scAB-ubc-nls-pcp-5xSunTag(SRv1-2P)

    Plasmid

    #185800

    Purpose

    one plasmid encoding scFv GFP and pcp-5xSunTag components for SunRISER SRv1

    Depositor

    Robin Lee

    Article

    Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

    Insert

    cmv-sfGFP-GB1-scAB--ubc-nls-pcp-5xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR

    Plasmid

    #170817

    Purpose

    Lentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Insert

    NLS-mNeonGreen-P2A-BlastR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pnYC-NpM-DmRoquin_702-819-del725-755-del790-812-GB1His6_AE

    Plasmid

    #148621

    Purpose

    Bacterial Expression of DmRoquin_702-819-del725-755-del790-812

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin_702-819-del725-755-del790-812 (roq Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pW401-lenti-sg1-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #189950

    Purpose

    Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg1-Itgb1) with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

    Insert

    NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pW402-lenti-sg2-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #189951

    Purpose

    Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg2-Itgb1) with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

    Insert

    NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pW403-lenti-sg3-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #189952

    Purpose

    Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg3-Itgb1) with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

    Insert

    NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGS403_scFv-GCN4-sfGFP-GB1-HRV3C-CENP-T[1-242 T11A, T85A] in AAVS donor

    Plasmid

    #211888

    Purpose

    CENP-T[1-242] transgene integration at AAVS1 safe harbor locus

    Depositor

    Iain Cheeseman

    Insert

    CENP-T[1-242] (CENPT Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    T11A, T85A

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c

    Plasmid

    #210508

    Purpose

    expresses NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c
    

    Use

    Tags

    GB1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pM162

    Plasmid

    #173221

    Purpose

    Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Inserts

    ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
    MCP-mCherry-GFP11-GB1
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMMDuet19

    Plasmid

    #45594

    Depositor

    Hideo Iwai

    Article

    Ellila et al FEBS Lett. 2011 Oct 10.

    Insert

    NpuDnaB mini intein
    

    Use

    Tags

    GB1 and H6-GB1

    Expression

    Bacterial

    Mutation

    removal of endonuclease domain

    Promoter

    T7

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSADuet502

    Plasmid

    #46373

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    NpuDnaB intein
    

    Use

    Tags

    GB1 and H6-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pICSL30028

    Plasmid

    #174587

    Purpose

    Level 0 N-terminal tag module carrying 6xHIS-GB1

    Depositor

    Mark Banfield

    Insert

    6xHIS-GB1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSEDuet40

    Plasmid

    #46378

    Depositor

    Hideo Iwai

    Article

    Ellila et al FEBS Lett. 2011 Oct 10.

    Insert

    TvoVMA intein
    

    Use

    Tags

    GB1 and H6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pOP3BT

    Plasmid

    #112603

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and GB1 fusions

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    Avi - 8His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pOP3BP

    Plasmid

    #112602

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and GB1 fusions

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    Avi - 8His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSABAD25-74

    Plasmid

    #46375

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    TvoVMA intein
    

    Use

    Tags

    H6-GB1 and SH3

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pOP5BT

    Plasmid

    #112609

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-GB1 and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pOP5BP

    Plasmid

    #112608

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-GB1 and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSABAD518

    Plasmid

    #46374

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    TvoVMA intein, inactive
    

    Use

    Tags

    H6-GB1 and SH3

    Expression

    Bacterial

    Mutation

    N186A, T+1A mutations, inactive

    Promoter

    pBAD

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMT1-24X-NOG(AtUBI10)

    Plasmid

    #202016

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Inserts

    dCas9-24XGP41
    NbGP41P-sfGFP-VP64-GB1
    

    Use

    Tags

    GB1 and sfGFP

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMT1-24X-NOG(CmYLCV)

    Plasmid

    #202014

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Inserts

    dCas9-24XGP41
    NbGP41P-sfGFP-VP64-GB1
    

    Use

    Tags

    GB1 and sfGFP

    Expression

    Plant

    Mutation

    Promoter

    CmYLCV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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42. MT-AvrXa10-FT

    Plasmid

    #234373

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

    Depositor

    Michael Smanski

    Article

    Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

    Inserts

    NbGP41-GFP-AvrXa10-GB1
    dCas9-24XGP41
    gRNAs targeting FT locus
    RFP
    

    Use

    Synthetic Biology

    Tags

    GB1, GFP, and GP41

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSKDuet16

    Plasmid

    #41684

    Depositor

    Hideo Iwai

    Article

    Ellila et al FEBS Lett. 2011 Oct 10.

    Insert

    NpuDnaE intein
    

    Use

    Tags

    GB1 and His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET30-GBFusion1

    Plasmid

    #133438

    Purpose

    Streptococcus GB1 domain in vector for bacterial expression.

    Depositor

    Gerhard Wagner

    Article

    Zhou et al J Biomol NMR. 2001 May . 20(1):11-4.

    Insert

    GB1 domain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    This fragment encodes only aa 1 to 56.

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pSADuet760

    Plasmid

    #45613

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    MjaTFIIB mini intein
    

    Use

    Tags

    GB1 and H6-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBHDuet40

    Plasmid

    #124358

    Purpose

    IPTG-inducible vector encoding the N-terminal gp41-1 split fragment with N-terminally His-tagged GB1

    Depositor

    Hideo Iwai

    Article

    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

    Insert

    gp41-1 N-intein
    

    Use

    Tags

    His6-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pBHBAD42

    Plasmid

    #124359

    Purpose

    Arabinose-inducible vector encoding the C-terminal gp41-1 split fragment fused to GB1

    Depositor

    Hideo Iwai

    Article

    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

    Insert

    gp41-1 C-intein
    

    Use

    Tags

    GB1 added during cloning

    Expression

    Bacterial

    Mutation

    Promoter

    Para

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. pHYDuet216A

    Plasmid

    #45611

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    NpuDnaE delta variant
    

    Use

    Tags

    GB1 and H6-GB1

    Expression

    Bacterial

    Mutation

    deleted T76, V97, D98, L100 from NpuDnaE intein

    Promoter

    T7

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. GEV 2

    Plasmid

    #12616

    Depositor

    John Louis

    Article

    Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

    Insert

    GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)
    

    Use

    Tags

    G protein and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

50. pPEPT1

    Plasmid

    #195001

    Purpose

    Production of peptides in E. coli as GB1 fusion

    Depositor

    Marko Hyvönen

    Article

    Pantelejevs et al Chem Sci. 2023 Nov 21;14(47):13915-13923

    Type

    Empty backbone

    Use

    Tags

    His8-tag and StrepTagII-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge