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pcDNA3-ΔN-RNMT
Plasmid#112708PurposeExpresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.DepositorInsertRNMT (RNMT Human)
UseTagsFLAG and HIV Rev nuclear export signal (NES)ExpressionMammalianMutationdeleted amino acids 2-120, mutated active-site D2…PromoterCMVAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
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HA tagged Sph ELL2
Plasmid#127270PurposeFor in vitro translation of human WT ELL2 with internal HA tagDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationWT Mets and HA tag at SphI site (8th Met)PromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
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HA tagged mid ELL2 M133, 138, 186ILeu
Plasmid#127276PurposeExpresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt functionDepositorInsertELL2 (ELL2 Human)
UseTagsExpressionMammalianMutationM133, 138, 186 to Ileu, HA tag at XbaI after Met …PromoterpEF1aAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
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cfSYFP2-N
Plasmid#110631PurposeCys-free yellow fluorescent protein for extracellular imaging. SYFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SYFP2.DepositorInsertcfSYFP2-N
UseTagsMulti-cloning sitesExpressionMammalianMutationCys48Ser and Cys70/MetPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3EF5a-PIK3CA H1047R
Plasmid#180034PurposeEntry vector of PIK3CA H1047R for gateway cloning.DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseEntry vectorTagsHA tagExpressionMammalianMutationH1047RPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-PIK3CA E545K
Plasmid#180032PurposeEntry vector of PIK3CA E545K for gateway cloning.DepositorInsertPIK3CA E545K (PIK3CA Human)
UseEntry vectorTagsHA tagExpressionMammalianMutationE545KPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJK687
Plasmid#71640PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met mutant (TdPurE2-K41M)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to methioninePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH-PIK3CA H1047R
Plasmid#180031PurposeThis plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseTagsHA tagExpressionMammalianMutationH1047RPromoterEF1aAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJK660
Plasmid#71635PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met/Phe79>Trp mutant (TdPurE2-K41M,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to methionine, phenylalanine-79…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK650
Plasmid#72400PurposeProduces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)DepositorInsertglutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
UseTagsExpressionBacterialMutationMutated tyrosine-397 to phenylalanine to block ad…PromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHM_YEAST
Plasmid#204231PurposeBacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM_YEAST (MDH1 Budding Yeast)
UseTags6X HisExpressionBacterialMutationDoes not include transit peptide and includes a M…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
HOXC6 (human) HIS-tag pET
Plasmid#8565DepositorInsertHOXC6 (HOXC6 Human)
UseTagsHis and T7ExpressionBacterialMutationThis protein starts at an internal Met initially …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHM1_ARATH
Plasmid#204236PurposeBacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM1_ARATH (mMDH1 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes done include the first 52 residues that are …PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHNP_ARATH
Plasmid#204238PurposeBacterial expression of Arabidopsis chloroplastic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHNP_ARATH (AT5G58330 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes not include the chloroplastic transit sequen…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT 6FLAG
Plasmid#182509PurposeC-SWAT compatible vector for C-terminal tagging with 6 FLAGDepositorInsert6 FLAG
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP
Plasmid#182510PurposeC-SWAT compatible vector for C-terminal tagging with GFPDepositorInsertGFP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_hRNMT_RAM
Plasmid#213528PurposeExpresses the human RNMT-RAM complexDepositorInsertsUseTagsHis6-sumoExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
HCMV UL97 KD
Plasmid#26688DepositorInsertUL97 (UL97 Human Cytomegalovirus (HHV-5))
UseTagsHAExpressionMammalianMutationKinase Deficient Mutant Lys 355 mutated to MetPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M1A(KaganF58)
Plasmid#52001PurposeExpresses the human MAVS CDS containing the point mutation M1ADepositorInsertMAVS
UseTagsExpressionMammalianMutationchanged Met 1 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET11a-dH2B
Plasmid#105501PurposeExpression of Drosophila histone H2B. An additional Ile codon was inserted after the initiating Met codon to enhance the protein expression levelDepositorInsertdH2B
UseTagsExpressionMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP CL1
Plasmid#182512PurposeC-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.DepositorInsertCL1 Degron
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HHV6 U69 KD
Plasmid#26690DepositorInsertU69 (U69 Human Herpes Virus 6)
UseTagsHAExpressionMammalianMutationKinase Deficient Mutant: Lys 218 mutated to MetPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT SMASh
Plasmid#182523PurposeC-SWAT compatible vector for C-terminal tagging with SMASh Degron. (Protease Cleavage Site: DEMEECSKHL).DepositorInsertSMASh Tag
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT 3MYC SMASh
Plasmid#182524PurposeC-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSKHL).DepositorInsertSMASh Tag
UseTags3MYCExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT 3MYC SMASh
Plasmid#182525PurposeC-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSQHL).DepositorInsertSMASh Tag
UseTags3MYCExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP DAmP
Plasmid#182511PurposeC-SWAT compatible vector for C-terminal tagging with GFP DAmP. GFP is immediately followed by the Hygromycin B (Hph) resistance cassette.DepositorInsertGFP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
UPR pBAM Ura3
Plasmid#45355DepositorInsertDHFR Ub Mek Ura3
UseTagsExpressionYeastMutationPromoterpCUP1Available sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
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C-SWAT AID*-GFP osTIR1
Plasmid#182514PurposeC-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.DepositorInsertAID*
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-MAVS-M142A(KaganF61)
Plasmid#51999PurposeExpresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"DepositorInsertMAVS
UseTagsHAExpressionMammalianMutationMet 142 to AlaPromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomSua1
Plasmid#107446PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertSua1
UseTagsV5-HisExpressionYeastMutationSee depositor comments below.PromoterGAL1Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTK084
Plasmid#171356PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTtRNA[Met] A.niger Pol-III terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK023
Plasmid#171295PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPtRNA[Met] P. rubens Pol-III promoter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-B0034-MV_RN
Plasmid#127017PurposeBasic Part RN- RBS+N-terminus; pET vector Ribosome Binding Site + Met-Val N-terminus start.DepositorInsertPart B0034m-MV_RN
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M1A-invitro(KaganE12)
Plasmid#52016PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation M1ADepositorInsertMAVS-M1A
UseTagsExpressionMammalianMutationchanged Met 1 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only
