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We narrowed to 1,640 results for: plasmid for e coli

Showing: 401 - 450 of 1640 results

  1. pBad-HPII TAG348

    Plasmid
    #105844
    Purpose
    expresses E. coli HPII catalase with TAG stop codon at site 348 and N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 348
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105844-plasmid-map-sequence-id-205537

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  2. pBad-HPII TAG283

    Plasmid
    #105843
    Purpose
    expresses E. coli HPII catalase with TAG stop codon at site 283 and N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 283
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105843-plasmid-map-sequence-id-205536

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  3. pBad-HPII TAG206

    Plasmid
    #105846
    Purpose
    expresses E. coli HPII catalase with TAG stop codon at site 206 and N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 206
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105846-plasmid-map-sequence-id-205551

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  4. pBad-HPII TAG568

    Plasmid
    #105845
    Purpose
    expresses E. coli HPII catalase with TAG stop codon at site 568 and N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 568
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105845-plasmid-map-sequence-id-205540

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  5. pSB3

    Plasmid
    #82027
    Purpose
    Hybrid McpC[1-350]-Tar[267-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-350]-Tar[267-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    82027-plasmid-map-sequence-id-175680

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  6. pSB2

    Plasmid
    #82026
    Purpose
    Hybrid McpC[1-286]-Tar[200-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-286]-Tar[200-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82026-plasmid-map-sequence-id-174389

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  7. pSB10

    Plasmid
    #82039
    Purpose
    Hybrid McpS[1-295]-Tar[200-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and P. putida chemoreceptor McpS: McpS[1-295]-Tar[200-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82039-plasmid-map-sequence-id-174336

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  8. pSB7

    Plasmid
    #82029
    Purpose
    Hybrid Tar[1-43]-CitA[55-173]-Tar[184-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: Tar[1-43]-CitA[55-173]-Tar[184-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82029-plasmid-map-sequence-id-174333

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  9. pSB9

    Plasmid
    #82038
    Purpose
    Hybrid ECA0434[1-385]-Tar[267-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and P. atrosepticum chemoreceptor ECA0434: ECA0434[1-385]-Tar[267-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82038-plasmid-map-sequence-id-174332

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  11. pDL14

    Plasmid
    #106901
    Purpose
    pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Inserts
    gam
    bet
    mutS
    lacI
    rhaR
    rhaS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Lysine 622 was replaced by Alanine
    Promoter
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    106901-plasmid-map-sequence-id-334155

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  12. pGBW-m4092694

    Plasmid
    #153907
    Purpose
    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153907-plasmid-map-sequence-id-307331

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  17. pMAL_dstβL

    Plasmid
    #73805
    Purpose
    Plasmid for selection for insertion and homodimerization
    Depositor
    Sarel Fleishman
    Article
    Elazar et al Elife. 2016 Jan 29;5. pii: e12125. doi:
    Inserts
    CAT Marker
    ToxR
    beta lactamase
    spectomycin resistance
    CLS TM domain
    Use
    Tags
    Part Of the dstbl chiemra, Part of the dsTbL chim…
    Expression
    Bacterial
    Mutation
    Promoter
    NaN and ToxR promotor
    Available since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pDEINO3

    Plasmid
    #179487
    Purpose
    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation
    Depositor
    Bogumil Karas
    Article
    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1
    Insert
    TetR/A
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    179487-plasmid-map-sequence-id-360433

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  22. pSEVA3b61

    Plasmid
    #58310
    Purpose
    Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.
    Depositor
    Tom Ellis
    Article
    Wright et al ACS Synth Biol. 2014 May 13.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    58310-plasmid-map-sequence-id-89822

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  24. p007ampGFP

    Plasmid
    #58535
    Purpose
    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T5 between two lac operators
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58535-plasmid-map-sequence-id-90574

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  25. p006kanGFP

    Plasmid
    #58534
    Purpose
    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T5 between two lac operators
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58534-plasmid-map-sequence-id-90573

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  26. pQE30del

    Plasmid
    #106902
    Purpose
    pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    106902-plasmid-map-sequence-id-327626

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  30. pSB1

    Plasmid
    #81227
    Purpose
    Hybrid Tar[1-43]-McpC[49-270]-Tar[184-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: Tar[1-43]-McpC[49-270]-Tar[184-553]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    81227-plasmid-map-sequence-id-174343

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  31. pDL17

    Plasmid
    #106483
    Purpose
    pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Inserts
    gam
    bet
    exo
    lacI
    rhaR
    rhaS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106483-plasmid-map-sequence-id-334156

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  38. pGBW-m4092686

    Plasmid
    #153895
    Purpose
    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Nearly native encoding;inactivating mutations in …
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153895-plasmid-map-sequence-id-307318

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  42. pSV014

    Plasmid
    #214740
    Purpose
    High copy thyA-infA plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation
    Depositor
    Tae Seok Moon
    Article
    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006
    Insert
    BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    214740-plasmid-map-sequence-id-427293

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  43. pSV013

    Plasmid
    #214739
    Purpose
    Medium copy thyA-infA plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation
    Depositor
    Tae Seok Moon
    Article
    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006
    Insert
    BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    214739-plasmid-map-sequence-id-427292

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  45. pTUM4

    Plasmid
    #230905
    Purpose
    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli
    Depositor
    Arne Skerra
    Article
    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-
    Inserts
    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    230905-plasmid-map-sequence-id-460506

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  48. AlkB-D135T

    Plasmid
    #160433
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Threonine; deleted t…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160433-plasmid-map-sequence-id-318950

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  49. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160434-plasmid-map-sequence-id-318954

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Showing: 401 - 450 of 1640 results