We narrowed to 10,200 results for: ESP

1. Ki67∆repeats-mNeonGreen-IRESpuro2

   Plasmid

   #183742

   Purpose

   Expresses Ki67∆repeats-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67(lacking repeats) (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   MKI67(lacking repeats)

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-deSpCas9-VP64-6xHis

   Plasmid

   #92117

   Purpose

   Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   T7

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC-H1-Esp3I-mU6-Kan

   Plasmid

   #213015

   Purpose

   Vector for Cloning of multiple gRNAs driven by distinct promoters (H1 and mU6)

   Depositor

   Stephanie Roessler

   Insert

   H1 promoter, mU6 promoter, gRNA scaffolds
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   H1 promoter

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRESpuro-Flag-Pol b(K72A)

   Plasmid

   #23258

   Depositor

   Robert Sobol

   Article

   Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

   Insert

   DNA polymerase beta (K72A) (POLB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K72A

   Promoter

   Available since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pX330-Flag-eSpCas9-D10A

   Plasmid

   #80449

   Purpose

   enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   ehSpCas9_D10A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-Flag-eSpCas9-H840A

   Plasmid

   #80454

   Purpose

   enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   ehSpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScalps eSpCas9_Zsgreen gRNA scaffold

   Plasmid

   #188449

   Purpose

   Expression of eSpCas9 tagged with Zsgreen

   Depositor

   Ajit Chande

   Article

   Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1-alpha

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-FLAG-eSpCas9-plus

   Plasmid

   #126774

   Purpose

   Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, R1060A; amino acids 1005-1013 replaced wit…

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

   Plasmid

   #141052

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   eSpCas9(1.1)-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mEmerald-Espin-like-ARD-C-18

    Plasmid

    #54088

    Purpose

    Localization: Actin Bundling, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Espin-like-ARD
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Ankyrin Repeat Domain

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pBP-P_TALEsp1_PUPsp1-lacI_Ptac-lacO

    Plasmid

    #204079

    Purpose

    Type 0 promoter part

    Depositor

    Mikhail Shapiro

    Insert

    TALEsp1-stabilized promoter driving lacI repressor; Ptac-lacO IPTG-inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJMC-L2-Reverse-Donor-Esp3I

    Plasmid

    #197844

    Purpose

    Donor vector to restore L2 destination via Esp3I (reverse orientation)

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. H2B-mNeonGreen_FRB(DmrC)_IRESpuro2

    Plasmid

    #182918

    Purpose

    Expesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2B

    Depositor

    Daniel Gerlich

    Article

    Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

    Insert

    pH2B (H2BC16P Human)
    

    Use

    Tags

    mNeonGreen-FRB(DmrC)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-L3-eSpCas9(1.1)-2A-EGFP-L2

    Plasmid

    #141051

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    eSpCas9(1.1)-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-R2L3-cmER-IRESpuro-pA

    Plasmid

    #139539

    Purpose

    Provides mouse ER protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    ER
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR-R2L3-cHA-IRESpuro-pA

    Plasmid

    #139537

    Purpose

    Provides HA protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-R2L3-cGR-IRESpuro-pA

    Plasmid

    #139538

    Purpose

    Provides GR protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    GR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459V2.0-eSpCas9(1.1)+K855A

    Plasmid

    #108298

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX459V2.0-eSpCas9(1.1)+K810A

    Plasmid

    #108297

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-Espin-Like-ARD-C-18

    Plasmid

    #62743

    Purpose

    Localization: Actin Bundling, C1 Cloning Vector, Excitation:587, Emission:610

    Depositor

    Michael Davidson

    Insert

    EspinLikeARD
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only