We narrowed to 16,808 results for: URE

1. BPK2101

   Plasmid

   #65770

   Purpose

   Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized SaCas9, and SaCas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS-3xFlag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYL112-19

   Plasmid

   #15459

   Depositor

   Chia Lee

   Article

   Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

   Insert

   S. aureus phage L54a integrase
   

   Use

   S.aureus-e. coli shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pSR1037

   Plasmid

   #208736

   Purpose

   Characterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)

   Depositor

   Lauren Andrews

   Article

   Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

   Insert

   PSarA1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSR1038

   Plasmid

   #208737

   Purpose

   Characterization plasmid for PCap (sfgfp expressed from PCap on the S. aureus pC194 replicon)

   Depositor

   Lauren Andrews

   Article

   Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

   Insert

   PCap
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCap

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-AsCas12f1-v5.1+T1-empty

   Plasmid

   #204977

   Purpose

   AsCas12f1-v5.1+T1-based Human cell genome editing

   Depositor

   Quanjiang Ji

   Article

   Wu et al Nature Catalysis (2023) 6, 695–709

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   N70Q/K103R/A104R/S118A/D364R

   Promoter

   PCMV,U6

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LacD_WT_pET21a

   Plasmid

   #29785

   Depositor

   SeWon Suh

   Article

   Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

   Insert

   Tagatose 1,6-diphosphate aldolase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

7. LacD_L165E_pET21a

   Plasmid

   #29786

   Depositor

   SeWon Suh

   Article

   Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

   Insert

   Tagatose 1,6-diphosphate aldolase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   L165E

   Promoter

   Available since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

8. LacD_L275S_pET21a

   Plasmid

   #29787

   Depositor

   SeWon Suh

   Article

   Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

   Insert

   Tagatose 1,6-diphosphate aldolase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   L275S

   Promoter

   Available since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pCasSA

   Plasmid

   #98211

   Purpose

   CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

   Depositor

   Quanjiang Ji

   Article

   Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTcI39-switch

    Plasmid

    #195557

    Purpose

    Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mRFP1
    mWasabi
    TcI39
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMPTY::sgRNA2

    Plasmid

    #165459

    Purpose

    Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

    Depositor

    Inigo Lasa

    Article

    Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

    Insert

    sgRNA2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SP01

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. BPK2139

    Plasmid

    #65776

    Purpose

    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJB38-NWMN2930

    Plasmid

    #84457

    Purpose

    pJB38 plasmid containing the S.aureus Newman genetic region between genes 29 and 30

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    Staphylococcus aureus NWMN genomic region NWMN29-30
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. LacD_L165E/L275S_pET21a

    Plasmid

    #29788

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    L165E and L275S

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

15. dCAS9-VP64_GFP

    Plasmid

    #61422

    Purpose

    Expresses dCAS9-VP64 activator with 2A GFP

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Concentrated Lentiviral Prep

    Insert

    dCAS9(D10A,H840A)-VP64_2A_GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    EF1A

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgRNA(MS2) cloning backbone

    Plasmid

    #61424

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. shRNA_circHUWE1_1

    Plasmid

    #215236

    Purpose

    Supression of shcircHUWE1(19,20)_1 expression

    Depositor

    Sefi Rosenbluh

    Article

    Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

    Insert

    circHUWE1 shRNA 1 (HUWE1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. shRNA_circHUWE1_2

    Plasmid

    #215235

    Purpose

    Supression of shcircHUWE1(19,20)_2 expression

    Depositor

    Sefi Rosenbluh

    Article

    Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

    Insert

    circHUWE1 shRNA 2 (HUWE1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-Flag-MIB1-IRES-Puro

    Plasmid

    #140240

    Purpose

    Lentiviral vector expressing flag-tagged MIB1

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    Flag-MIB1 (MIB1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut

    Plasmid

    #187154

    Purpose

    mammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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