We narrowed to 351 results for: lenticrispr v2

1. lentiCRISPR.v2.TetON_Cas13d_U6_DR

   Plasmid

   #196727

   Purpose

   Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.

   Depositor

   Bernhard Schmierer

   Insert

   RfxCas13d-T2A-miRFP670nano
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   TRE

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRv2_PROX1_1+3

   Plasmid

   #121176

   Purpose

   Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

   Depositor

   Timo Otonkoski

   Insert

   Cas9-P2A-puro and U6 promoters driven expression of gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TLCV2 sgAAVS1

   Plasmid

   #127099

   Purpose

   Encodes gRNA for human AAVS1.

   Depositor

   Adam Karpf

   Insert

   AAVS1 (PPP1R12C AAVS1 Human) (PPP1R12C Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPRv2_PROX1_1+4

   Plasmid

   #121177

   Purpose

   Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

   Depositor

   Timo Otonkoski

   Insert

   Cas9-P2A-puro and U6 promoters driven expression of gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPRv2R

   Plasmid

   #167932

   Purpose

   lentiCRISPRv2 variant with mScarlet-I fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPRv2-Opti

   Plasmid

   #163126

   Purpose

   LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiGuide-TagRFP-2A-BSD

   Plasmid

   #167930

   Purpose

   Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiGuide-TagBFP-2A-BSD

   Plasmid

   #167929

   Purpose

   Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-DAG1

   Plasmid

   #205149

   Purpose

   Overexpress DAG1

   Depositor

   Monkol Lek

   Insert

   Dystroglycan 1 (DAG1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Carries common missense variant c.41C>G (p.Ser…

   Promoter

   EF-1a

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPRv2-Blast-mU6

    Plasmid

    #206806

    Purpose

    pLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassette

    Depositor

    Jolanda Van Leeuwen

    Article

    Unlu et al Genome Med. 2023 Oct 12;15(1):78. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. GESTALT_pX330-v1

    Plasmid

    #103061

    Purpose

    plasmid px330 with guide targeting GESTALT v1 through V5 constructs

    Depositor

    Jay Shendure

    Article

    McKenna et al Science. 2016 May 26. pii: aaf7907.

    Insert

    integration of Cas9 with guide targeting GESTALT barcodes V1 to V5
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MCP-ADAR2-DD

    Plasmid

    #170124

    Purpose

    Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MCP-ADAR2-DD-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a core promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Neg. Ctrl_sgRNA

    Plasmid

    #111298

    Purpose

    CRISPR KO murine neg. ctrl.

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    NegCtrl sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MsCLYBL_sgRNA1

    Plasmid

    #111294

    Purpose

    CRISPR KO murine CLYBL

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    sgRNA1 against murine CLYBL
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. MsCLYBL_sgRNA6

    Plasmid

    #111295

    Purpose

    CRISPR KO murine CLYBL

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    sgRNA6 against murine CLYBL
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. MsMUT_sgRNA1

    Plasmid

    #111296

    Purpose

    CRISPR KO murine MUT

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    sgRNA1 against murine MUT
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. MsMUT_sgRNA3

    Plasmid

    #111297

    Purpose

    CRISPR KO murine MUT

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    sgRNA3 against murine MUT
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. TLCV2-APOBEC1-YTH

    Plasmid

    #178949

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTH-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. HiFi Cas9

    Plasmid

    #188490

    Purpose

    Expresses FLAG tagged HiFi Cas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R691A

    Promoter

    EF1a

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgCtr- LentiCRISPRv2

    Plasmid

    #107402

    Purpose

    Lentiviral expression of Cas9 and a control gRNA

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    control gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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