19,771 results

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pAAV-syn-iGluSnFR4s-NGR-WPRE

Plasmid

#234437

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Article

Aggarwal et al bioRxiv 2025.03.20.643984

Has Service

AAV1

Insert

iGluSnFR4s-NGR

Use

AAV

Tags

IgK chain and Myc epi tag-NGR GPI anchor

Expression

Mammalian

Promoter

Synapsin

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCAX APP 695

Plasmid

#30137

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein (APP Human)

Expression

Mammalian

Mutation

wild type splice variant 695

Available Since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-rM3D(Gs)

Plasmid

#45549

Purpose

Expresses rM3D (Gs) neuronal activator

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

rM3D (Gs)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST-eGFP-C1-VPS35

Plasmid

#163622

Purpose

human VPS35 cDNA C-terminally tagged with eGFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

human VPS35 (VPS35 Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-NPM1wt-IRES-mCherry

Plasmid

#131824

Purpose

Lentiviral plasmid expressing NPM1 wildtype (human) in mammalian cells upon transduction

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Lentiviral

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

Plasmid

#205565

Purpose

Express human NEDD4L (delta C2 isoform)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

NEDD4L (NEDD4L Human)

Use

Piggybac

Expression

Mammalian

Mutation

delta C2 isoform

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pER-mScarlet3_N1

Plasmid

#189772

Purpose

Construct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

ER-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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Flag_HsIRE1a_pBabePuro

Plasmid

#54337

Purpose

N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector

Depositor

David Ron

Article

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

The endogenous IRE1a signal sequence (aa1-18) was…

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7A

Plasmid

#28047

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Tags

2xHA and EGFP

Expression

Mammalian

Available Since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-mCherry-flex-dtA

Plasmid

#58536

Purpose

Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

Depositor

Naoshige Uchida

Article

Wu et al Nature. 2014 May 15;509(7500):325-30. do

Insert

dtA (DIP_RS18250 )

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-KRAB-dCas9-P2A-mCherry

Plasmid

#60954

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Insert

KRAB-dCas9-P2A-mCherry fusion

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, KRAB domain, and mCherry

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-1c

Plasmid

#26802

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

SREBP-1c (SREBF1 Human)

Tags

2XFLAG

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6Xhis-human SUMO 3

Plasmid

#133772

Purpose

express 6Xhis- SUMO 3 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 3 (SUMO3 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pKeima-LiveDrop

Plasmid

#199695

Purpose

lipophagy reporter system based on the Keima-fluorophore

Depositor

Tobias Walther

Article

Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110.

Insert

GPAT4 (Gpat4 Fly)

Tags

Keima

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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anti-FLAG M2 light chain

Plasmid

#175358

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 light chain

Depositor

Joost Snijder

Article

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 light chain

Tags

N-terminal secretion signal peptide (MEAPAQLLFLLL…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-puro-cOVA-IRES-BFP

Plasmid

#135074

Purpose

Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.

Depositor

Maria Castro

Insert

cOVA-IRES-BFP (OVAL Chicken)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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PB-TO-hNGN2

Plasmid

#172115

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

hNGN2 (NEUROG2 Human)
puroR-T2A-mycNLS-mTagBFP2

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-Blasticidin

Plasmid

#75085

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pPalmitoyl-mTurquoise2

Plasmid

#36209

Purpose

In vivo visualization of the membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

p63(1-29) (ARHGEF25 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pMRX-IP TFEB-sfGFP

Plasmid

#135402

Purpose

Expresses TFEB-sfGFP

Depositor

Eisuke Itakura

Article

Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

Insert

TFEB (TFEB Human)

Use

Retroviral

Tags

sfGFP

Expression

Mammalian

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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