We narrowed to 24,475 results for: crispr

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pYC2085

Plasmid

#158721

Purpose

CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

Sth1 sgRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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PX383 Lactobacillus johnsonii Cas9

Plasmid

#68320

Purpose

Lactobacillus johnsonii Cas9

Depositor

Feng Zhang

Insert

LjCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

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AAVi U6_gRNA CMV_SadCas9-KRAB

Plasmid

#214609

Purpose

All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

Depositor

Ralf Gilsbach

Article

Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

Inserts

gRNA
SadCas9

Use

AAV

Tags

NP NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, N580A

Promoter

CMV and U6

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS443-pCSDest2-AcrIIC3Nme

Plasmid

#85713

Purpose

Mammalian expression of Type II-C anti-CRISPR protein AcrIIC3Nme from Neisseria meningitidis

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type II-C anti-CRISPR AcrIIC3Nme

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

Plasmid

#149722

Purpose

Lentiviral expression of crRNA with GFP and a neomycin resistance gene

Depositor

Junwei Shi

Article

Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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scFv-sfGFP-DNMT3A1

Plasmid

#102278

Purpose

The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

Depositor

Margaret Goodell

Article

Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

Insert

DNMT3A1 (DNMT3A Human)

Use

Lentiviral

Tags

scFV-sfGFP

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAH243

Plasmid

#121147

Purpose

Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast

Depositor

Katsunori Tanaka

Article

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Inserts

humanized Streptococcus pyogenes Cas9
gRNA expression module

Use

CRISPR

Tags

Flag

Expression

Yeast

Promoter

S.pombe rrk1 and nmt41

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pPB9v1

Plasmid

#210028

Purpose

Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_053

Plasmid

#113591

Purpose

Constitutive expression of a single sgRNA and the fluorophore violet-excited GFP in immune cells

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Commun. 2019 Apr 10;10(1):1668. doi:

Insert

sgRNA cloning site

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

Plasmid

#83306

Purpose

Lentivirus vector to express guideRNA and dCas9 with puro resistant gene

Depositor

Tohru Kimura

Article

Murakami et al Nat Immunol. 2021 Mar;22(3):301-311. doi

Insert

sgRNA and dCas9 from pX330

Use

Lentiviral

Tags

FLAG and PA tags

Expression

Mammalian

Promoter

U6 for sgRNA and CBh for dCas9

Available Since

Dec. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA3717

Plasmid

#240491

Purpose

CRISPRi-mediated Knockdown of RPA3717

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA3701

Plasmid

#240490

Purpose

CRISPRi-mediated Knockdown of RPA3701

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA1580

Plasmid

#240488

Purpose

CRISPRi-mediated Knockdown of RPA1580

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-dCas9-T2A-GFP

Plasmid

#193781

Purpose

Tricistronic expression of dCas9-EGFP-BSD

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

dead Cas9

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

In frame with existing Cas9 promoter

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-3XGFPnls

Plasmid

#75385

Purpose

PCP-3XGFPnls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

PCP

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

EFS

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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FUCas9Cyan

Plasmid

#85554

Purpose

Expresses Cas9 with fluorescent Cyan reporter

Depositor

Marco Herold

Insert

humanised S.pyrogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

hUBC

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBBK23 pCAS-Tyr-[gRNA: 6=ARS416] (SplitHygR, AmpR)

Plasmid

#179007

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, AmpR)

Plasmid

#179006

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK17 pCAS-Tyr-[gRNA: 7=HIS3] (SplitKanR, AmpR)

Plasmid

#179001

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK16 pCAS-Tyr-[gRNA: 6=ARS416] (SplitKanR, AmpR)

Plasmid

#179000

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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