We narrowed to 11,985 results for: pcdna

1. pcDNA-dCas9-FLp300

   Plasmid

   #61356

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, Human, S. pyogenes)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT-HA-hM3D(Gq)

   Plasmid

   #45547

   Purpose

   Expresses hM3D (Gq) neuronal activator

   Depositor

   Bryan Roth

   Article

   Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

   Insert

   hM3D (Gq)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Hyg-mEGFP

   Plasmid

   #191847

   Purpose

   Expresses the monomeric form of EGFP

   Depositor

   Bertrand Collet

   Article

   Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1436 pcDNA3 Flag HA

   Plasmid

   #10792

   Purpose

   pcDNA3 vector with added Flag, HA tags

   Depositor

   William Sellers

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3/HA-SUMO3 (Sentrin 2)

   Plasmid

   #17361

   Purpose

   Mammalian expression of human SUMO3 with HA tag

   Depositor

   Edward Yeh

   Article

   Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

   Insert

   SUMO3 (SUMO3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. L444P GBA pcDNA3.1

   Plasmid

   #188582

   Purpose

   Ectopic expression L444P GBA

   Depositor

   Anthony Schapira

   Article

   Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

   Insert

   GBA (GBA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L444P

   Promoter

   CMV

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Flag-RNF5

   Plasmid

   #122043

   Purpose

   express RNF5

   Depositor

   Ze'ev Ronai

   Insert

   RNF5 (RNF5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5-FRT-TO-Nsp16-HF

   Plasmid

   #157725

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp16-HF (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pCDNA3 Flag MKK7B2Jnk2a2

   Plasmid

   #19727

   Depositor

   Roger Davis

   Article

   Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

   Insert

   pCDNA3 FlagMKK7B2Jnk2a2 (MAPK9, Map2k7 Human, Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-SARS-Spike

    Plasmid

    #145031

    Purpose

    Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV Spike
    

    Use

    Tags

    C9 and CD5 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 16, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA5-FRT-TO-Nsp16

    Plasmid

    #157723

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp16 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. K44A HA-dynamin 2 pcDNA3.1

    Plasmid

    #34685

    Depositor

    Sandra Schmid

    Insert

    dynamin 2ba
    

    Use

    Tags

    HA (variant)

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-Flag-Med25

    Plasmid

    #64771

    Purpose

    Mammalian protein expression of flag-tagged Med25

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25 (MED25 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA4-GFP-Vinculin

    Plasmid

    #107153

    Purpose

    mammalian expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    (His)10-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.3 Gag-imCherry

    Plasmid

    #208882

    Purpose

    codes for Gag from HIV-1 with an internal mCherry fluorescent protein

    Depositor

    Raphael Gaudin

    Article

    Diana Brychka et al bioRxiv

    Insert

    HIV-1 Gag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Cyto-CaNAR2

    Plasmid

    #64729

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2-NES
    

    Use

    Tags

    Cerulean3, Nuclear export signal (NES), and YPet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3 MKK7b1(K149A)

    Plasmid

    #14674

    Depositor

    Roger Davis

    Article

    Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

    Insert

    MKK7b1(K149A) (Map2k7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lys-149Ala

    Promoter

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-VEGFR3-V5/HIS

    Plasmid

    #205618

    Purpose

    Expression of human VEGFR3 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    VEGFR3 receptor tyrosine kinase (FLT4 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    T28M, S455R- Please see depositor comment

    Promoter

    CMV

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ELP-TEMP/pcDNA3.1(-)

    Plasmid

    #178434

    Purpose

    Genetically encoded ratiometric fluorescent thermometer

    Depositor

    Takeharu Nagai

    Article

    Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

    Insert

    ELP-TEMP
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Xiap-Myc

    Plasmid

    #11833

    Depositor

    Guy Salvesen

    Article

    Takahashi et al J Biol Chem. 1998 Apr 3. 273(14):7787-90

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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