We narrowed to 2,067 results for: ENDOG

1. LLP788_Traptavidin-EZH2-FL-CO

   Plasmid

   #211781

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to polycomb repressive complex subunit EZH2, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-EZH2
   

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect EZH2 e…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. HR-TERT-promotor

   Plasmid

   #71398

   Purpose

   Homologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Promoter

   Endogenous TERT promotor

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLP784_SnoopCatcher-DNMT3A-CO

   Plasmid

   #211777

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Spy-EZH2
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect EZH2 e…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUF1-dCas9-Sir2a

   Plasmid

   #122510

   Purpose

   Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.

   Depositor

   Lubin Jiang

   Article

   Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

   Insert

   dCas9-Sir2a
   

   Use

   CRISPR

   Tags

   3*FLAG

   Mutation

   dCas9(D10A,H840A)

   Promoter

   PfHsp86

   Available Since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LLP787_SnoopCatcher-EZH2-FL-CO

   Plasmid

   #211780

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-DNMT3A
   

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LLP780_SpyCatcher-KRAB-CO

   Plasmid

   #211773

   Purpose

   SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SpyCatcher-KRAB
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect KRAB e…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Th-tdTomato

   Plasmid

   #159460

   Purpose

   Targeting vector to TH gene locus for induction of P2A-fused tdTomato, with floxed PGK-puroR drug resistant cassette

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Insert

   P2A-tdTomato
   

   Use

   Cre/Lox

   Tags

   P2A

   Expression

   Mammalian

   Promoter

   Endogenous TH

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTriEX-Antenna-GDI Cdc42

   Plasmid

   #101681

   Purpose

   "Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

   Depositor

   Klaus Hahn

   Article

   Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

   Insert

   Cdc42
   

   Tags

   6xHis and FRET Antenna (mCerulean-cpVenus)

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

   Plasmid

   #204288

   Purpose

   yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

   Depositor

   Linda Huang

   Article

   Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

   Insert

   pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
   

   Tags

   mRuby3

   Expression

   Yeast

   Promoter

   pHis3

   Available Since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKnockinDonor-humanCTCF-miniAID-mClover3

    Plasmid

    #136883

    Purpose

    knockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCF

    Depositor

    Chunliang Li

    Article

    Hyle et al Nucleic Acids Res. 2019 Jul 26;47(13):66

    Insert

    CTCF-knockin-homology arm-miniAID-mClover3
    

    Tags

    miniAID-mClover3

    Expression

    Mammalian

    Promoter

    no promoter

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP779_αGCN4-VP64-CO

    Plasmid

    #211772

    Purpose

    SunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-VP64
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect VP64 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LLP790_αGCN4-EZH2-FL-CO

    Plasmid

    #211784

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SnoopCatcher-KRAB
    

    Tags

    2xOLLAS

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LLP789_αGCN4-DNMT3A-CO

    Plasmid

    #211783

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-DNMT3A
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Sox17(2A-phiC31o)

    Plasmid

    #140762

    Purpose

    Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.

    Depositor

    Hideyuki Okano

    Article

    Serizawa et al Development. 2019 Nov 4;146(21). pii: de

    Insert

    phiC31o
    

    Use

    Mouse Targeting

    Promoter

    Endogenous Sox17

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pONSY-mCherry

    Plasmid

    #111874

    Purpose

    This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Sox1(2A-Cre)

    Plasmid

    #140760

    Purpose

    Targeting vector to Sox1 gene locus for induction of P2A-fused Cre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(WT) sequence.

    Depositor

    Hideyuki Okano

    Article

    Serizawa et al Development. 2019 Nov 4;146(21). pii: de

    Insert

    Cre
    

    Use

    Mouse Targeting

    Promoter

    Endogenous Sox1

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Nanog-CreER targeting construct

    Plasmid

    #59720

    Purpose

    Cre-ER knockin targeting construct for mouse endogenous Nanog locus

    Depositor

    Jacob Hanna

    Article

    Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

    Insert

    creERT2
    

    Use

    Mouse Targeting

    Promoter

    mNanog

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pONSY-Venus

    Plasmid

    #111875

    Purpose

    This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Venus
    

    Use

    Capsaspora owczarzaki

    Tags

    Venus

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJ207 Delta-cpc-KanR

    Plasmid

    #51468

    Purpose

    Deletes the cpc operon in Synechocystis and confers kanamycin resistance

    Depositor

    Anastasios Melis

    Article

    Kirst et al Biochim Biophys Acta. 2014 Oct;1837(10):

    Insert

    Replacing the cpc operon with kanamycin resistance
    

    Use

    Synthetic Biology

    Promoter

    Synechocystis endogenous cpc-operon promoter

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEFU6-EGFP_shPKA-Cα

    Plasmid

    #114142

    Purpose

    Expression of shRNA for knocking down endogenous PKA-Cα. EGFP is also expressed.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    shRNA expression for knock down mouse PKA-Cα
    

    Expression

    Mammalian

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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