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167,150 results

Showing: 1801 - 1820 of 167150 results

  1. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  3. TFORF2329

    Plasmid
    #143186
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CIZ1 (CIZ1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143186-plasmid-map-sequence-id-397832

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  4. pFA6a-HaloTag-KanMX6

    Plasmid
    #87029
    Purpose
    Tag S. pombe genes in C terminal with HaloTag
    Depositor
    Julien Berro
    Insert
    HaloTag
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87029-plasmid-map-sequence-id-173001

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  6. pTFE3.1.0-gDNA

    Plasmid
    #112473
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFE3
    Depositor
    Kevin White
    Insert
    TFE3 (TFE3 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112473-plasmid-map-sequence-id-230504

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  7. pTFE3-donor

    Plasmid
    #112377
    Purpose
    CRISPR donor plasmid to tag human transcription factor TFE3 with GFP
    Depositor
    Kevin White
    Insert
    TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
    Use
    CRISPR
    Mutation
    hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112377-plasmid-map-sequence-id-229742

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  9. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

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  10. pCBC-DT1T2

    Plasmid
    #50590
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, U626t plus, U629p, T2
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50590-plasmid-map-sequence-id-210724

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  14. pET His6 MBP TEV LIC cloning vector (1M)

    Plasmid
    #29656
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis, MBP, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only
    29656-plasmid-map-sequence-id-237947

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Showing: 1801 - 1820 of 167150 results