We narrowed to 3,255 results for: actin

1. TK-GFP-Nluc-P2A-neo-TK

   Plasmid

   #134896

   Purpose

   Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Insert

   TK-GFP-Nluc-P2A-Neo-TK
   

   Use

   Cryptosporidium expression

   Promoter

   Cryptosporidium actin and enolase

   Available Since

   Nov. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MultiMate-Rainbow

   Plasmid

   #206252

   Purpose

   Expression of 7 fluorescently labelled proteins in mammalian cells. Can be used to generate recombinant baculovirus particles.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   H2B (human); Actin, Tubulin (B. taurus); mito mCherry, GST mTagBFP1 (Synthetic); CyOFP1 (E. quadricolor); Ctnnb1 (mouse)
   

   Use

   Recombinant baculovirus production (bac-to-bac)

   Tags

   iRFP713

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHLmMBP-3

   Plasmid

   #72345

   Purpose

   Mammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Tags

   6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

   Expression

   Mammalian

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Blast-mNeon-ACTB

   Plasmid

   #207751

   Purpose

   Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-SMS2-TEV-Twin-Strep

   Plasmid

   #202529

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SGMS2 (SGMS2 Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TGA)

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pExpreS2-1-C3-PA-10H-SIII

   Plasmid

   #175443

   Purpose

   HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Tags

   3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

   Expression

   Insect

   Promoter

   Fused Actin-HSP70

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Puro-mNeon-ACTB

   Plasmid

   #207752

   Purpose

   Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIG-FGFRdn

   Plasmid

   #80431

   Purpose

   Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos

   Depositor

   Aixa Morales

   Article

   Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

   Insert

   Fibroblast growth factor receptor 1 (FGFR1 Chicken)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains aa 1–425

   Promoter

   beta-actin promoter

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pBa-KIF5C 559-tdTomato-FKBP

   Plasmid

   #64211

   Purpose

   KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

   Insert

   Kif5c (Kif5c Rat)
   

   Tags

   FKBP and tdTomato

   Expression

   Mammalian

   Mutation

   motor domain aa1-559

   Promoter

   chicken Beta Actin

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBa-tdTomato-flag-BicD2 594-FKBP

    Plasmid

    #64205

    Purpose

    Bicaudal D protein aa1-594 (excluding start Met) with N-terminal tandem dimer Tomato and flag tags and C-terminal FKBP

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    Bicaudal D protein (Bicd2 Mouse)
    

    Tags

    FKBP, flag, and tdTomato

    Expression

    Mammalian

    Mutation

    no start methionine, aa1-594

    Promoter

    chicken Beta Actin

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHLmMBP-6

    Plasmid

    #72346

    Purpose

    Mammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP

    Expression

    Mammalian

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQCXIH BRGI-Flag

    Plasmid

    #19148

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pCAX-mScarlet-FBP17

    Plasmid

    #178366

    Purpose

    Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells

    Depositor

    Erik Dent

    Article

    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

    Insert

    Formin Binding Protein 17 (FNBP1 Human)
    

    Expression

    Mammalian

    Mutation

    400 Glutamic Acid changed to Aspartic acid (403 A…

    Promoter

    Chicken Beta Actin

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MultiMate 5 colors

    Plasmid

    #206266

    Purpose

    Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
    

    Use

    Recombinant baculovirus production, cre acceptor…

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBa-eGFP-flag-BicD2 594-FKBP

    Plasmid

    #63569

    Purpose

    Bicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBP

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    Bicaudal D protein (Bicd2 Mouse)
    

    Tags

    FKBP, eGFP, and flag

    Expression

    Mammalian

    Mutation

    no start methionine, aa1-594

    Promoter

    chicken Beta Actin

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG668 lifeAct-pFAST

    Plasmid

    #172866

    Purpose

    Expresses LifeAct-pFAST in mammalian cells (actin labeling)

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    LifeAct-pFAST
    

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cas12f-GE ver4.0

    Plasmid

    #176543

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver4.0
    Cas12f-GE ver4.0
    

    Expression

    Mammalian

    Promoter

    U6 and chicken β-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-mitf

    Plasmid

    #69801

    Purpose

    Used to make mitf knockout porcine cell line

    Depositor

    Jianguo Zhao

    Article

    Wang et al Sci Rep. 2015 Aug 21;5:13348. doi: 10.10

    Inserts

    mitf-sgRNA
    SpCas9
    

    Expression

    Mammalian

    Promoter

    Chicken Beta-Actin and U6

    Available Since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHLmMBP-2

    Plasmid

    #72344

    Purpose

    Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP

    Expression

    Mammalian

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cas12f-GE ver3.0

    Plasmid

    #176508

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver3.0
    Cas12f-GE ver3.0
    

    Expression

    Mammalian

    Promoter

    U6 and chicken β-actin

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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