8,811 results

1. pENTR-GFP-MDC1 (770-7)

   Plasmid

   #26284

   Depositor

   Eric Campeau

   Insert

   Mediator of DNA damage checkpoint 1 (MDC1 Human)
   

   Use

   Entry vector

   Tags

   GFP

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHes1(2.5k)-luc

   Plasmid

   #43806

   Depositor

   Ryoichiro Kageyama

   Article

   Takebayashi et al J Biol Chem. 1994 Feb 18;269(7):5150-6.

   Insert

   Hes1 Promoter (Hes1 Mouse)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Contains the murine Hes1 promoter

   Promoter

   Hes1 Promoter

   Available Since

   March 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLv-CMV-MyoD-ER(T)

   Plasmid

   #26809

   Depositor

   Jeffrey Chamberlain

   Article

   Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

   Insert

   MyoD-ER(T) (Myod1 Mouse)
   

   Use

   Lentiviral

   Available Since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pDONR223-PDGFRA

   Plasmid

   #23892

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PDGFRA (PDGFRA Human)
   

   Use

   Gateway donor vector

   Mutation

   M260I

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti-EF1a-dCas9-KRAB-Puro

   Plasmid

   #99372

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-KRAB-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-GFP

   Plasmid

   #74165

   Purpose

   Mammalian expression of EGFP

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   GFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1

   Plasmid

   #72266

   Purpose

   Express gene of interest under EF-1 promoter

   Depositor

   Kazuhiro Oka

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124429

   Purpose

   Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(TEV cleavage seq) T2A mCherry
   

   Expression

   Mammalian

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28:GFP

   Plasmid

   #60733

   Purpose

   pET28 driving expression of GFP, biobrick part Bba_E0040

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Pam-FL

    Plasmid

    #42570

    Depositor

    Vijaya Ramesh

    Article

    Santos et al J Neurosci Res. 2006 Feb 1;83(2):222-32.

    Insert

    human MYCBP2 (MYCBP2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Pam-FL clone lacks alternatively spliced exons 52…

    Promoter

    CMV

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSET FlipSuc-90u delta1

    Plasmid

    #14475

    Depositor

    Wolf Frommer

    Article

    Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

    Insert

    FlipThuE W283A
    

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    N192D, W283A, 13 amino acids deleted from linkers

    Promoter

    T7

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-EGFP-C1-HsRCK

    Plasmid

    #25033

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    Rck (DDX6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCXB-EBNA1

    Plasmid

    #41857

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    EBNA-1 (EBNA-1 human herpesvirus 4)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCM433

    Plasmid

    #15670

    Depositor

    Christopher Marx

    Article

    Marx et al BMC Res Notes. 2008 Feb 26;1:1. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector

    Available Since

    Aug. 31, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. SpyTag003-sfGFP

    Plasmid

    #133454

    Purpose

    Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-sfGFP
    

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCA24N-p50-ligase

    Plasmid

    #87742

    Purpose

    IPTG-inducible expression of p50-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis and human p50/NF-kB (amino acids 40-366)

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Cre-IRES2-GFP

    Plasmid

    #26646

    Purpose

    Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

    Depositor

    Anjen Chenn

    Article

    Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    GFP and IRES2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET30-2-GAPDH

    Plasmid

    #83910

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-C1 mCherry-NLS

    Plasmid

    #58476

    Purpose

    Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Insert

    3X NLS with 3X stop codons
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV mGAD65(delE1) GFP WPRE SV40pA

    Plasmid

    #177316

    Purpose

    To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

    Depositor

    Hirokazu Hirai

    Article

    Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

    Insert

    mGAD65(delE1) promoter, GABAergic interneurons specific promoter
    

    Use

    AAV

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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