We narrowed to 2,892 results for: Rho;

1. pAAV-hSyn-soCoChR-mCardinal

   Plasmid

   #107713

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. hSynapsin promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Insert

   soCoChR-mCardinal
   

   Use

   AAV

   Tags

   KA2 and mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-GFP-EBA175-Flag

   Plasmid

   #154970

   Purpose

   Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   GFP and signal peptide (residues 1 to 32) from TG…

   Expression

   Mammalian

   Mutation

   insert codes for EBA175 residues 1284 to 1462

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pODN-AAVS1-mR2-CAAX

   Plasmid

   #183871

   Purpose

   Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-CAAX

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   CMV

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV hSyn1 CaRhAC T2A tDimer

   Plasmid

   #101722

   Purpose

   - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

   Depositor

   Thomas Oertner

   Article

   Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

   Inserts

   CatRhAC
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mutation

   E497K,C569D

   Promoter

   human Synapsin1 promotor and ribosomal skip seque…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-GFP-TRIM28

   Plasmid

   #185010

   Purpose

   Encodes gRNA for mouse TRIM28 along with Cas9 with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   TRIM28 sgRNA (Trim28 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-HypaCas9-mR2-TUBA1B_sgRNA

   Plasmid

   #183888

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   TUBA1B sgRNA spacer (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJS699

   Plasmid

   #168779

   Purpose

   Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

   Depositor

   Timothy Whitehead

   Article

   Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

   Insert

   Spike RBD (S betacoronavirus)
   

   Use

   Tags

   Cmyc

   Expression

   Mutation

   N343Q

   Promoter

   GAL1

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 20XUAS IVS Jaws mCherry_tr

   Plasmid

   #111549

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

   Depositor

   Vivek Jayaraman

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws_mCherry_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Opto-GPR27

   Plasmid

   #106013

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR27; Opto-GPR27; B3)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR27 (GPR27 Human, Bovine)
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMKO shRNA Bad

    Plasmid

    #17237

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bad shRNA (BAD Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)

    Plasmid

    #114101

    Purpose

    fusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

    Insert

    Gaussia luciferase, M23; Volvox Channelrhodopsin 1; EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-Prom1 D-5

    Plasmid

    #184017

    Purpose

    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.

    Depositor

    Peter Stoilov

    Article

    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

    Insert

    Prom1 SV8(-Ex19a) D-5 (Prom1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of exon 19a (amino acids 696-701), and d…

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. 10XUAS IVS Jaws mVenus_tr

    Plasmid

    #111553

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

    Depositor

    Vivek Jayaraman

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws_mVenus_tr
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. 13XLexAop2 IVS Jaws mCherry_tr

    Plasmid

    #111551

    Purpose

    Optogenetics in Drosophila: LexAop2-induced expressionof Jaws mCherry

    Depositor

    Vivek Jayaraman

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws_mCherry_trafficked
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-C/EBPbZIP (pGEX-bZIPa)

    Plasmid

    #12435

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

    Insert

    68 a.a. fragment of C/EBP alpha (CEBPA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

16. mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

    Plasmid

    #173866

    Purpose

    Mammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Opto-GPR150

    Plasmid

    #106050

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR150; Opto-GPR150; E4)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR150 (GPR150 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-45b-NFYC-IDR-mEGFP

    Plasmid

    #185015

    Purpose

    Bacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    NFYC IDR (Nfyc Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-EF1alpha-soCoChR-GFP

    Plasmid

    #107709

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMKO shRNA Bmf

    Plasmid

    #17236

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bmf shRNA (BMF Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only