We narrowed to 3,255 results for: actin

1. pME-Lifeact-EGFP-3xHA (JDW 1322)

   Plasmid

   #224494

   Purpose

   Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Liefact-EGFP-3xHA
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

   Plasmid

   #132686

   Purpose

   AAV plasmid encoding mouse ACY1 with C-terminal flag tag

   Depositor

   Jonathan Long

   Article

   Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

   Insert

   Acylase I (Acy1, Acy1 Mouse)
   

   Use

   AAV

   Tags

   Flag

   Promoter

   Chicken b-actin

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-SMS1-TEV-Twin-Strep

   Plasmid

   #202528

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SGMS1 (SGMS1 Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TAA)

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Puro-mScarlet-ACTB

   Plasmid

   #207754

   Purpose

   Donor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. mIFP12-sEspin-C-18

   Plasmid

   #56262

   Purpose

   Localization: Actin Bundling, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   sEspin
   

   Tags

   mIFP12

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pGBT-R16

   Plasmid

   #31827

   Depositor

   Stephen Ekker

   Article

   Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

   Insert

   mini Tol2 ITR
   

   Use

   Cre/Lox; Gene trap

   Tags

   mRFP

   Mutation

   mRFP lacks start codon

   Promoter

   beta Actin

   Available Since

   Sept. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pS23.U6<ActB.mus_C-term-KI_gRNA

   Plasmid

   #207408

   Purpose

   gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   ActB
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pExpreS2-1-CR-PA-10H-SIII

   Plasmid

   #175447

   Purpose

   HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Tags

   BiP Secretion Peptide and Thrombin-Protein A-10Hi…

   Expression

   Insect

   Promoter

   Fused Actin-HSP70

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ArpC3-FLAG_pLib

   Plasmid

   #173680

   Purpose

   ArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

   Depositor

   Roberto Dominguez

   Article

   Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

   Insert

   ArpC3 (ARPC3 Human)
   

   Tags

   TEV cleavage site and FLAG tag

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ArpC1_pLib

    Plasmid

    #173678

    Purpose

    ArpC1 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC1 (ARPC1B Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMADM-alpha

    Plasmid

    #36890

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    Neo
    ATG (T)
    GFP
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, deleted…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP

    Plasmid

    #89061

    Purpose

    AAV-gRNA targeting the murine Ldlr gene

    Depositor

    William Lagor

    Article

    Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

    Insert

    EmGFP
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    Chicken beta actin with partial CMV enhancer

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG470 LifeAct-iFAST

    Plasmid

    #130821

    Purpose

    Expresses LifeAct-iFAST in mammalian cells (actin labeling)

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    LifeAct-iFAST
    

    Tags

    myc-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. LK4-Ets2

    Plasmid

    #44606

    Depositor

    Robert Oshima

    Article

    Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

    Insert

    Ets2 (Ets2 Mouse)
    

    Expression

    Mammalian

    Promoter

    human Beta-Actin

    Available Since

    May 13, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pMini-CAAGs::RFP-DR48

    Plasmid

    #132640

    Purpose

    Fluorescent reporter to quantify elicitation of the strand annealing mediated repair pathway

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    mRFP
    

    Expression

    Mammalian

    Mutation

    Contains a 92bp insertion interrupting the open …

    Promoter

    Chicken B-actin

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. ArpC2_pLib

    Plasmid

    #173679

    Purpose

    ArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC2 (ARPC2 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. exon L-firefly

    Plasmid

    #81006

    Purpose

    luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

    Depositor

    Richard Baines

    Article

    Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

    Insert

    paralytic (para Fly)
    

    Tags

    firefly luciferase

    Expression

    Insect

    Mutation

    spanning exon 24 to exon 26, termination codon in…

    Promoter

    P-AC5 (Actin 5C)

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGFP AH2 tensin

    Plasmid

    #50514

    Purpose

    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Article

    Pankov et al J Cell Biol. 2000 Mar 6;148(5):1075-90.

    Insert

    Tensin (TNS1 Chicken)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    AA659--761, including actin homology 2 domain of …

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. 1184_pAAV-U6-ApoB-gRNA2-CB-EmGFP

    Plasmid

    #89062

    Purpose

    AAV-gRNA targeting the murine Apob gene

    Depositor

    William Lagor

    Article

    Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

    Insert

    EmGFP
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    Chicken beta actin with partial CMV enhancer

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-Lifeact-mRuby2-3xMyc (JDW 1246)

    Plasmid

    #242554

    Purpose

    Gateway middle entry clone containing Lifeact-mRuby2-3xMyc; Red F-actin reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mRuby2
    

    Use

    Gateway subcloning

    Available Since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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