We narrowed to 7,177 results for: tet-on

1. pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

   Plasmid

   #160570

   Purpose

   A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   sgRNA:scaffold tetraloop MS2 aptamer SAM
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

   Plasmid

   #121804

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

   Plasmid

   #121808

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GB1-His-TET1-878-911-L900A(pL91)

   Plasmid

   #124395

   Purpose

   E.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-L900A (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   L900A

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GB1-His-TET1-878-911-T898E(pL91)

   Plasmid

   #124394

   Purpose

   E.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-T898E (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   T898E

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. GB1-His-TET1-878-911-T898A(pL91)

   Plasmid

   #124393

   Purpose

   E.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-T898A (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   T898A

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. GB1-His-TET1-878-911-L897A(pL91)

   Plasmid

   #124392

   Purpose

   E.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-L897A (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   L897A

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. GB1-His-TET1-878-911-I894A(pL91)

   Plasmid

   #124391

   Purpose

   E.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-I894A (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   I894A

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-Tet-off-uniRapR-mCherry-Vav2(DPZ)

   Plasmid

   #91874

   Purpose

   Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Insert

   Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2 (VAV2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLacO1-BCD7-tetR-ssrA P42U18C45T13 v40-5a (497)

    Plasmid

    #70733

    Purpose

    plasmid for building 5n1

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Insert

    tetR-ssrA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQacR-BCD7-tetR-ssrA P68U18C45T13 v40-5 (569)

    Plasmid

    #70739

    Purpose

    plasmid for building 5n2

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Insert

    tetR-ssrA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

    Plasmid

    #221235

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tet-off Full length Tau (0N4R, P301L/V337M)

    Plasmid

    #188573

    Purpose

    Dox-repressible expression of the 0N4R Tau isoform

    Depositor

    Franz-Ulrich Hartl

    Article

    Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

    Insert

    0N4R Tau (MAPT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P301L and V337M

    Promoter

    pTight TRE

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid

    #183411

    Purpose

    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    Tet-on 3G
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP

    Plasmid

    #199360

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-SUMOEu-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

    Plasmid

    #184395

    Purpose

    Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Firefly luciferase beta-globin
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On 3G

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199363

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

    Plasmid

    #199351

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

    Plasmid

    #184396

    Purpose

    Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase beta-globin
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On 3G

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

    Plasmid

    #184397

    Purpose

    Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Firefly luciferase beta-globin-PTC39
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    PTC in beta-globin at amino acid position 39

    Promoter

    Tet-On 3G

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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