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pC1-HyPerFLEX-ERlum

Plasmid

#238941

Purpose

Mammalian expression of HyPerFLEX targeted to the luminal side of the endoplasmic reticulum membranes

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-ERlum (Calreticulin signal sequence-HyPerFLEX-KDEL)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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NanoBRET Assay Vector, NanoLuc-MEK1

Plasmid

#238578

Purpose

Express NanoLuc-MEK1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

MEK1 (MAP2K1 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-puro_UCOE_TRE3VG_mCherry-Responder

Plasmid

#230053

Purpose

It presents the UCOE sequence and the TRE3VG inducible promoter allowing a more stable dox-dependent inducible activation of mCherry though the OPTi-OX platform across differentiation of hiPSCs

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

UCOE

Expression

Mammalian

Promoter

TRE3VG

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSIG2

Plasmid

#226909

Purpose

MoClo-compatible vectors for dsRNA production in E. coli

Depositor

Li Hung Chen

Article

Wu et al Plant Methods. 2025 Jul 11;21(1):96. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-DeltaER

Plasmid

#238416

Purpose

Expresses GRP78 lacking the ER signal peptide in mammalian cells

Depositor

Amy Shiu Lee

Article

Liu et al J Biol Chem. 2025 Feb 28;301(4):108369.

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

The first 18 amino acids which is the ER signal p…

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLX313_PELO_WT

Plasmid

#228938

Purpose

Constitutive expression of wild-type human PELO

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO (PELO Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLViN-iRFP670-α-cateninA+ΔβH

Plasmid

#229707

Purpose

Lentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

amino acids 670-673, RAIM--> GSGS

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLViN-iRFP670-α-cateninΔβH

Plasmid

#229706

Purpose

Lentiviral expression of iRFP670-alpha-catenin-delta-beta-H in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PX459-CTNNA1(α-catenin CRISPR KO)

Plasmid

#229708

Purpose

Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.8D2Q.Tau2N4R

Plasmid

#226398

Purpose

Expresses his-tagged, 3C-cleavable 8D2Q mutant 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

His.3C

Expression

Bacterial

Mutation

8D2Q.Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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