We narrowed to 14,241 results for: Cas9

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xCas9(3.6)-ABE(7.10)

Plasmid

#108387

Purpose

Mammalian expression vector for xCas9(3.6)-ABE

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.6)-ABE(7.10)

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

Plasmid

#71828

Purpose

Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

Depositor

Vlatka Zoldos

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

BACH2-sgRNA8 (BACH2 S. pyogenes, Synthetic, Human)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

U6

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

Plasmid

#222992

Purpose

Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNA500

Plasmid

#149576

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-evoCjCas9-ABE8e_gRNA

Plasmid

#202561

Purpose

hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

AAV

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pH-Ubi-miniSdd7-nCas9-PBE

Plasmid

#204856

Purpose

For cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd7-nSpCas9(D10A)-UGI

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Promoter

ZmUbi-1, OsU3, 35S

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA2

Plasmid

#99735

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pJ607-CMV-GFP-2A-Cas9

Plasmid

#154830

Purpose

GFP-2A-Cas9 (CHO codon-optimized)

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

GFP-2A-Cas9
HygR

Use

CRISPR

Expression

Mammalian

Mutation

Codon-optimized (Cricetulus griseus)

Promoter

CMV and SV40 promoter

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pB[nos-Cas9 PUb-ZsGreen]

Plasmid

#169011

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Expression

Insect

Promoter

Drosophila melanogaster nanos

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA P1

Plasmid

#80943

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA P1 for Pd-l1 binding.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pQdCas9.luxR(mut)-sggfp

Plasmid

#236185

Purpose

The plasmid pQdCas9.luxR(mut)-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the GFP gene. Additionally, this plasmid contains a mutation in the luxR gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

Quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene

Promoter

Quorum sensing promoter

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-nCas9-UdgX

Plasmid

#163566

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

Plasmid

#163561

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-HF-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pUbi_dCas9-BFP-KRAB-gRNA

Plasmid

#221503

Purpose

CRISPRi machinery fish codon optimized expressing plasmid

Depositor

Alessandro Ori

Insert

dCas9-tagBFP-KRAB

Use

Fish expression

Mutation

D10A and H839A point mutations

Promoter

Zebrafish Ubiquitin promoter

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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OA-986F (AAEL003877-dCas9-VPR)

Plasmid

#183993

Purpose

Expresses dCas9-VPR under the Ae. aegypti polyubiquitin promoter

Depositor

Omar Akbari

Article

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

AAEL003877-dCAS9-VPR

Use

CRISPR

Tags

dCas9-VPR

Expression

Insect

Promoter

AAEL003887

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pACT1:Cas9-GFP, U6:sgTK

Plasmid

#122852

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgTK

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA M3

Plasmid

#80936

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

Plasmid

#148859

Purpose

Mammalian Expression of HsXRN1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsXRN1-sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-evoSpCas9-NLS-6xHis

Plasmid

#207390

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAC153-dual-dCas9VP96-sgExpression

Plasmid

#48239

Purpose

Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP96

Expression

Mammalian

Mutation

D10A H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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