We narrowed to 7,283 results for: Tet-on

1. pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA)

   Plasmid

   #231603

   Purpose

   Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-4x(6xAAA) under CMV promoter

   Depositor

   Marvin Tanenbaum

   Article

   Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

   Insert

   Tornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA)
   

   Expression

   Mammalian

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized+8xAAA

   Plasmid

   #231604

   Purpose

   Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-8xAAA under CMV promoter

   Depositor

   Marvin Tanenbaum

   Article

   Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

   Insert

   Tornado-1xALFATag-5xSunTag_codon_optimized-8xAAA
   

   Expression

   Mammalian

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag-Pseudoknot

   Plasmid

   #231593

   Purpose

   Express circular RNA translation reporter with 2xALFATag-10xSunTag-Pseudoknot under H1 promoter

   Depositor

   Marvin Tanenbaum

   Article

   Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

   Insert

   Tornado-2xALFATag-10xSunTag-Pseudoknot sequence
   

   Expression

   Mammalian

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+

   Plasmid

   #225649

   Purpose

   tetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

   Depositor

   Andre Sanches Ribeiro

   Article

   Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

   Insert

   tetR/tetA, araBAD and LacO3O1 in tandem conformation
   

   Tags

   mCherry

   Expression

   Bacterial

   Promoter

   tetA, araBAD and LacO3O1 promoters

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

   Plasmid

   #177360

   Purpose

   AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Inserts

   tdTomato
   Chimeric guide for SaCas9
   

   Use

   AAV

   Tags

   P2A and a tetracycline-controlled transactivator …

   Expression

   Mammalian

   Promoter

   Synapsine promoter and U6 promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

   Plasmid

   #177365

   Purpose

   AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Inserts

   tdTomato
   Chimeric guide for SaCas9
   

   Use

   AAV

   Tags

   P2A and a tetracycline-controlled transactivator …

   Expression

   Mammalian

   Promoter

   CMV and U6 promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUW-tetO-mCh-Gata2-del-N-ZF(287-342)

   Plasmid

   #193068

   Purpose

   doxycycline-inducible expression of mouse Gata2 without the N-terminal zinc finger (amino acids 287-342), in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

   Insert

   mCh-Gata2-del-N-ZF(287-342)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUW-tetO-mCh-Gata2-del-C-ZF(343-379)

   Plasmid

   #193069

   Purpose

   doxycycline-inducible expression of mouse Gata2 without the C-terminal zinc finger (amino acids 343-379), in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

   Insert

   mCh-Gata2-del-C-ZF(343-379)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUW-tetO-mCh-Gata2-del-N-Terminal(1-235)

   Plasmid

   #193067

   Purpose

   doxycycline-inducible expression of mouse Gata2 without the N-terminal (amino acids 1-235), in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

   Insert

   mCh-Gata2-del-N-Terminal(1-235)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔCARD

    Plasmid

    #194608

    Purpose

    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus CARD Domain

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus Sequence Encoding Met 1 to Lys 176 (Casp9 Mouse)
    

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Deletion of N-terminal CARD domain and changed Cy…

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tet-Inducible Human Caspase-9 Alpha C287A Mutant

    Plasmid

    #194709

    Purpose

    Inducible Expression of Human Caspase-9 Alpha C287A Mutant

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    Human Caspase-9 C287A Mutant (CASP9 Human)
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine 287 to Alanine to eliminate prot…

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tet-inducible 3xFlag Mouse C325A Caspase-9 Mutant

    Plasmid

    #194501

    Purpose

    Inducible Expression of N-Terminal 3X FLAG Mouse Caspase-9 C325A Mutant

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    Caspase-9 C325A Mutant (Casp9 Mouse)
    

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Changed Cysteine 325 to Alanine to eliminate prot…

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid

    #193318

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4MYC N-terminal tagging
    

    Tags

    4MYC

    Expression

    Bacterial

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid

    #193317

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    

    Tags

    4FLAG

    Expression

    Bacterial

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag

    Plasmid

    #186710

    Purpose

    N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

    Depositor

    Paula Tamagnini

    Article

    Mascia et al Green Chemistry, 24 (2022):6156

    Insert

    alr4766
    

    Use

    Synthetic Biology

    Tags

    His-Tag (6His)

    Promoter

    Ptrc.x.tetO2

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLB37_PB_pGK-H2B-mIFP-T2A-rTetR-HDAC4-zeo

    Plasmid

    #179440

    Purpose

    Expresses rTetR-HDAC4(human) for gene silencing, integration by Piggybac

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    H2B-mIFP-T2A-rTetR-HDAC4
    

    Expression

    Mammalian

    Promoter

    pGK

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. TetO-FUW-FLAG-NLS-EnR-MCS_N-term

    Plasmid

    #136884

    Purpose

    Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    engrailed repressor domain (en Fly)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TetO-FUW-FLAG-NLS-VP64-MCS_N-term

    Plasmid

    #136886

    Purpose

    Doxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    VP64 activator domain
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. TetO-FUW-FLAG-NLS-KRAB-MCS_N-term

    Plasmid

    #136888

    Purpose

    Doxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    KRAB repressor domain
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

    Plasmid

    #160570

    Purpose

    A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNA:scaffold tetraloop MS2 aptamer SAM
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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