We narrowed to 4,947 results for: mcs

1. Dynamin I-K44A-non-tagged

   Plasmid

   #32695

   Depositor

   Robert Lefkowitz

   Article

   Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

   Insert

   Dynamin I (Dnm1 Rat)
   

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   CMV

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

2. HSC1-D4Z4-GiP

   Plasmid

   #58542

   Purpose

   Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter

   Depositor

   James Ellis

   Article

   Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

   Inserts

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   D4Z4 repeat, partial
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   Human EF1-alpha

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-TSV (linearized in VP2)

   Plasmid

   #70032

   Purpose

   pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region

   Depositor

   Mariet Feltkamp

   Article

   van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

   Insert

   TSV full genome, linearized in VP2 gene
   

   Use

   Cloning vector

   Expression

   Bacterial

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 782 pMEActMEK

   Plasmid

   #31166

   Depositor

   Nathan Lawson

   Article

   Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

   Insert

   MEK (MAP2K1 Human)
   

   Use

   Entry vector

   Mutation

   Ser218Glu Ser222Asp; activated MEKK

   Available Since

   Aug. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pJL1-EPA-DNNNS-DQNRT

   Plasmid

   #128397

   Purpose

   In vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Exotoxin A
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   242DNNNS246 and 384DQNRT388 engineered glycosylat…

   Promoter

   T7

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-uTEV1-ACBD5

   Plasmid

   #171008

   Purpose

   HiLITR protease with ACBD5 C-terminal targeting information (peroxisome)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-ACBD5(tmd)
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. (517) SB KRAB-SadCas9 U6-gStop

   Plasmid

   #163023

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Promoter

   Tet-On

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5A0-T

   Plasmid

   #92016

   Purpose

   Expresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease

   Depositor

   Brian Pfleger

   Article

   Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

   Inserts

   TALE targeting lac operator
   Tobacco Etch Virus Protease
   

   Use

   TALEN

   Tags

   5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

   Mutation

   S219V

   Promoter

   J23102 Promoter (from Anderson promoter library) …

   Available Since

   Jan. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-uTEV1-SQStmd

   Plasmid

   #171009

   Purpose

   HiLITR protease with SQS C-terminal targeting information (ER)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-SQS(tmd)
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET_RGG-RGG_withTEVsite

    Plasmid

    #124940

    Purpose

    Expression of RGG-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    with TEV site between RGG domains

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-EF1a-tetR-T2A-tSapphire

    Plasmid

    #176312

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    T-sapphire
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET_RGG-RFP-RGG

    Plasmid

    #124944

    Purpose

    Exoression of RGG-RFP-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His and mCherry

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Pmyo-3::tag:SL1(G20A)

    Plasmid

    #81114

    Purpose

    Expression of exogenous SL1(G20A) in C. elegans muscle

    Depositor

    Xiao Liu

    Article

    Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

    Insert

    sls-1.2 (sls-1.2 Nematode)
    

    Tags

    illumina-adapter

    Expression

    Worm

    Mutation

    G20A

    Promoter

    promoter of myo-3

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. 360 pCR kdrb

    Plasmid

    #13067

    Depositor

    Nathan Lawson

    Article

    Covassin et al Proc Natl Acad Sci U S A. 2006 Apr 25. 1

    Insert

    kdrb (kdr Zebrafish)
    

    Available Since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pMS1088

    Plasmid

    #80159

    Purpose

    pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

    Depositor

    Marcelo Sousa

    Article

    Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

    Insert

    HisSUMO-Cys-4xNuG2-Ald
    

    Tags

    HisSUMO

    Promoter

    T7

    Available Since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

    Plasmid

    #182354

    Purpose

    Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

    Plasmid

    #182356

    Purpose

    Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI-TPI_WT-xrRNA-4MS2-4H

    Plasmid

    #108370

    Purpose

    Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type TPI reporter with 4MS2 binding sites downstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJL1-PorA-4xDQNAT

    Plasmid

    #128392

    Purpose

    In vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    PorA
    

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJL1-TTc-4xDQNAT

    Plasmid

    #128393

    Purpose

    In vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin fragment C domain
    

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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