We narrowed to 23,171 results for: crispr

1. pGTag Hygro BFP nLAP -1

   Plasmid

   #194324

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(BFP)
   

   Use

   Bacterial cloning vector

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGTag Hygro EGFP nLAP -1

   Plasmid

   #194321

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(EGFP)
   

   Use

   Bacterial cloning vector

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGTag Hygro BFP nLAP -0

   Plasmid

   #194323

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(BFP)
   

   Use

   Bacterial cloning vector

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGTag Hygro EGFP nLAP -0

   Plasmid

   #194320

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(EGFP)
   

   Use

   Bacterial cloning vector

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGTag Hygro BFP nLAP -2

   Plasmid

   #194325

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(BFP)
   

   Use

   Bacterial cloning vector

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGTag Hygro BFP nLAP +1

   Plasmid

   #194318

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(BFP)
   

   Use

   Bacterial cloning vector

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGTag Hygro BFP nLAP +2

   Plasmid

   #194319

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(BFP)
   

   Use

   Bacterial cloning vector

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGTag Hygro EGFP nLAP +1

   Plasmid

   #194306

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(EGFP)
   

   Use

   Bacterial cloning vector

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGTag Hygro EGFP nLAP +2

   Plasmid

   #194307

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(EGFP)
   

   Use

   Bacterial cloning vector

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pICSL1P4_AtU626Ter26

    Plasmid

    #191772

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICSL1P7_AtU626Ter26

    Plasmid

    #191775

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICSL1P2_AtU626Ter26

    Plasmid

    #191770

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pICSL1P5_AtU626Ter26

    Plasmid

    #191773

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pICSL1P6_AtU626Ter26

    Plasmid

    #191774

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NAX_CAGGS_CAS9_VPR_mcherry

    Plasmid

    #167894

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

16. pCJH026_proD_Cas12c-multiplexing_CAM

    Plasmid

    #183073

    Purpose

    Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSB700 lenti gRNA Hygro_zhang2.0

    Plasmid

    #167915

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSB700 lenti gRNA EYFP_zhang2.0

    Plasmid

    #167914

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NAX_CAGGS_CAS9_VPR_iRFP713

    Plasmid

    #167892

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NAX_CAGGS_CAS9_VPR_Hygro

    Plasmid

    #167891

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. NAX_CAGGS_CAS9_VPR_EBFP2

    Plasmid

    #167889

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NAX_CAGGS_CAS9_VPR_Blasto

    Plasmid

    #167888

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEc-crRNA2

    Plasmid

    #170089

    Purpose

    encodes E. coli type I-E single spacer array

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    E. coli type I-E single spacer array
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    J23119

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RPL13A_PQR_mScarlet_NOL_LoxP_PQR_zeo_PQR_MCS_RPL13A-3'UTR

    Plasmid

    #165043

    Purpose

    Repair template for CAS9 complex genome editing

    Depositor

    Brian Chen

    Article

    Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

    Insert

    RPL13A (RPL13A Human)
    

    Expression

    Bacterial

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBzCas13b-gRNA-2

    Plasmid

    #164859

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-2
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBzCas13b-gRNA-3

    Plasmid

    #164860

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-3
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. OA-1050R (Ubiq-dCasRx)

    Plasmid

    #132417

    Purpose

    Express dCasRx under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    dCasRx
    

    Expression

    Insect

    Promoter

    Ubiq

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. OA-1050L (UASt-CasRx)

    Plasmid

    #132418

    Purpose

    Express CasRx under UASt promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Inserts

    CasRx
    UASt+hsp70
    

    Expression

    Insect

    Promoter

    UASt

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSM-dCas9::Ap

    Plasmid

    #154307

    Purpose

    Shuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers.

    Depositor

    I-Son Ng

    Article

    Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti

    Plasmid

    #138312

    Purpose

    Lenti expression of AcrIIA14, C-terminus

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA14, C terminus
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-Himar-dCas9-mammalian

    Plasmid

    #138414

    Purpose

    Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Tags

    3xFLAG, 6xHis, and SV40 NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFETCh_ZHX3_iso1

    Plasmid

    #128239

    Purpose

    Donor vector for 3' FLAG tag of human ZHX3_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZHX3_iso1 Homology arms
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFETCh_SSX2B

    Plasmid

    #128241

    Purpose

    Donor vector for 3' FLAG tag of human SSX2B

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    SSX2B Homology arms
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFETCh_ZNF326_iso1

    Plasmid

    #128236

    Purpose

    Donor vector for 3' FLAG tag of human ZNF326_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF326_iso1 Homology arms
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFETCh_ZBTB49_iso1

    Plasmid

    #128240

    Purpose

    Donor vector for 3' FLAG tag of human ZBTB49_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZBTB49_iso1 Homology arms
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAW228

    Plasmid

    #113242

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Expression

    Bacterial

    Mutation

    TTGGTAG->tAggtag

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAW227

    Plasmid

    #113241

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Expression

    Bacterial

    Mutation

    TTGGTAG->Atggtag

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMAZ.1.0-gDNA

    Plasmid

    #112455

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MAZ

    Depositor

    Kevin White

    Insert

    MAZ (MAZ Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTHRB.1.0-gDNA

    Plasmid

    #112429

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor THRB

    Depositor

    Kevin White

    Insert

    THRB (THRB Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. dSp-Rta 100-175

    Plasmid

    #99667

    Purpose

    Expresses dSp Cas9 fused to truncated RTA activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated RTA activation domain (101-175)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. dSp-Rta 50-175

    Plasmid

    #99665

    Purpose

    Expresses dSp Cas9 fused to truncated RTA activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated RTA activation domain (51-175)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAW218

    Plasmid

    #113240

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Expression

    Bacterial

    Mutation

    AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAW217

    Plasmid

    #113239

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Expression

    Bacterial

    Mutation

    GGTAG->ggtaC

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAW215

    Plasmid

    #113237

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Expression

    Bacterial

    Mutation

    GGTAG->ggAag

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAW214

    Plasmid

    #113236

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Expression

    Bacterial

    Mutation

    GGTAG->gCtag

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAW213

    Plasmid

    #113235

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Expression

    Bacterial

    Mutation

    GGTAG->Cgtag

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAW211

    Plasmid

    #113233

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGA

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Expression

    Bacterial

    Mutation

    GGTAG->AACGA

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAW216

    Plasmid

    #113238

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Expression

    Bacterial

    Mutation

    GGTAG->ggtTg

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pSPgRNA

    Plasmid

    #47108

    Purpose

    Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    SPgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. lenti dCAS-VP64_Blast

    Plasmid

    #61425

    Purpose

    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Lentiviral Prep

    Insert

    dCAS9(D10A, N863A)-VP64_2A_Blast
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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