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We narrowed to 62,913 results for: cloning

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Showing: 1861 - 1880 of 62913 results

  9. STING-V1

    Plasmid
    #124262
    Purpose
    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus1
    Expression
    Mammalian
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    124262-plasmid-map-sequence-id-241476

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  10. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    107943-plasmid-map-sequence-id-207614

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  13. pcDNA3.1-ccdB-Nanoluc

    Plasmid
    #87067
    Purpose
    Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Available since
    March 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13 Articles
    87067-plasmid-map-sequence-id-192710

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  20. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-498727

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Showing: 1861 - 1880 of 62913 results