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Showing: 2821 - 2850 of 3549 results

  1. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid
    #176261
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Expression
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176261-plasmid-map-sequence-id-346586

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  2. Bxb1Donor_G315T_ADRB2_LucReporter

    Plasmid
    #129793
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant G315T of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Sriram Kosuri
    Article
    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    G315T
    Promoter
    N/A
    Available since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129793-plasmid-map-sequence-id-255170

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  3. Bxb1Donor_W99A_ADRB2_LucReporter

    Plasmid
    #129789
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Sriram Kosuri
    Article
    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    W99A
    Promoter
    N/A
    Available since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129789-plasmid-map-sequence-id-255165

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  4. Bxb1Donor_N51L_ADRB2_LucReporter

    Plasmid
    #129790
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant N51L of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Sriram Kosuri
    Article
    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    N51L
    Promoter
    N/A
    Available since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129790-plasmid-map-sequence-id-255172

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  8. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73037
    Purpose
    Express Nluc and copGFP dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    73037-plasmid-map-sequence-id-136876

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  9. pCDH-EF1S-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73032
    Purpose
    Express fluorescent and luminescence dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated EF1
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73032-plasmid-map-sequence-id-136029

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  10. pDEST26-hTICAM1-Nluc

    Plasmid
    #197634
    Purpose
    Human TRIF protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TICAM1 (TICAM1 Human)
    Use
    Tags
    NanoLuciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197634-plasmid-map-sequence-id-417624

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  11. pcDNA3.1-EOLA1/CXORF40A

    Plasmid
    #180776
    Purpose
    Expresses EOLA1/CXORF40A in mammalian cells
    Depositor
    Douglas Feldman
    Insert
    EOLA1 (EOLA1 Human)
    Use
    Tags
    SBP-Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    180776-plasmid-map-sequence-id-356523

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  13. pSVOA-delta5-mcfos-luc

    Plasmid
    #22244
    Depositor
    Ananda Roy
    Insert
    c-fos (Fos Mouse)
    Use
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only

  14. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

    Plasmid
    #197264
    Purpose
    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197264-plasmid-map-sequence-id-389660

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  16. pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

    Plasmid
    #197265
    Purpose
    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197265-plasmid-map-sequence-id-389723

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  22. pINSPECT_SigP:NLuc

    Plasmid
    #197263
    Purpose
    Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197263-plasmid-map-sequence-id-389724

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  23. pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73040
    Purpose
    Express Nluc and copGFP dual reporter in mammalian cells
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK (phosphoglycerate kinase 1)
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73040-plasmid-map-sequence-id-136039

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  24. pDEST26-Nluc-hTRAF3

    Plasmid
    #197645
    Purpose
    Human CAP1 protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TRAF3 (TRAF3 Human)
    Use
    Tags
    NanoLuciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197645-plasmid-map-sequence-id-392624

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  29. TRUPATH Triple (bidirectional) GasS

    Plasmid
    #196055
    Purpose
    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196055-plasmid-map-sequence-id-385866

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Showing: 2821 - 2850 of 3549 results