We narrowed to 6,273 results for: nls

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RNAPII_CTD27-52_N2A_S1966C

Plasmid

#98682

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A and S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

N1775A, N1782A, S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc NES-mCE

Plasmid

#82463

Purpose

Expression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt Mouse, HIV)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pTSK450

Plasmid

#34552

Depositor

James McNally

Insert

MS2-CP-NLS

Tags

mCherry

Expression

Yeast

Promoter

Met25

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_FL_WT

Plasmid

#98651

Purpose

Expresses MBP-tagged full length FUS

Depositor

Nicolas Fawzi

Article

Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgRNA

Plasmid

#124844

Purpose

Vector for Cre-dependent expression of SaCas9

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SaCas9, gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS and NLS-3xHA

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD

Plasmid

#98669

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMIIS928

Plasmid

#238211

Purpose

Expresses TaTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Tags

HA-NLS

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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PB-CAG-CasRX-P2A-EGFP-BGH PolyA

Plasmid

#154004

Purpose

Express CasRx

Depositor

Hui Yang

Article

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

CasRx

Use

CRISPR

Promoter

CAG

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Hs.cargo(Cas9)

Plasmid

#174864

Purpose

Expresses human Peg10 cargoRNA encoding SpCas9

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

SpCas9 flanked by HsPeg10 UTRs

Tags

HA-SV40 NLS and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJ4M_hnRNPA2_FL_WT

Plasmid

#139109

Purpose

Express FL hnRNPA2 with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

none

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pC035 - dLwCas13a-msfGFP

Plasmid

#91925

Purpose

Expresses catalytically inactive LwCas13a for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

dLwCas13a

Use

CRISPR

Tags

NLS-3xHA and NLS-msfGFP

Expression

Mammalian

Mutation

R488A and R1060A

Promoter

EF-1a

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pC015 - dLwCas13a-NF

Plasmid

#91905

Purpose

Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

dLwCas13a

Use

CRISPR

Tags

NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

Expression

Mammalian

Mutation

R474A and R1046A

Promoter

CAG

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCR1316

Plasmid

#111126

Purpose

Expresses FANCA.

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCA (FANCA Human)

Use

Lentiviral

Tags

HA

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-bArrestin2-TevC-P2A-TdTomato

Plasmid

#89869

Purpose

expresses iTango bArrestin2-TevC component and TdTomato bicistronically

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

bArrestin2-TevC-P2A-TdTomato

Use

Synthetic Biology

Expression

Mammalian

Mutation

R173H in TdTomato (please see depositor comments …

Promoter

CMV

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16

Plasmid

#89870

Purpose

expresses DRD2-iTango component in mammalian cells

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

DRD2-V2tail-TevN-BLITz1-TetR-VP16

Expression

Mammalian

Mutation

short isoform of DRD2 (deletion of 242-270)

Promoter

CMV

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pJRH029

Plasmid

#171060

Purpose

Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

Gag-spyCas9

Use

CRISPR and Lentiviral

Tags

2x SV40 NLS and 3x FLAG tag

Expression

Mammalian

Promoter

CAG

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pdCas9-humanized

Plasmid

#44246

Purpose

Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dead Cas9 with 3X NLS

Use

CRISPR and Retroviral; Vector

Tags

3x NLS

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

MSCV LTR promoter

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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dCas9 plasmid

Plasmid

#100091

Purpose

dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Type

Empty backbone

Use

CRISPR

Tags

3XFlag-NLS-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pC034 - LwCas13a-msfGFP-2A-Blast

Plasmid

#91924

Purpose

Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR and Lentiviral

Tags

NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMIIS1011

Plasmid

#238212

Purpose

Expresses ParTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

ParTasR

Tags

HA-NLS

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-FLEX-eNpHR-EYFP

Plasmid

#89876

Purpose

To generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

eNpHR=EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

TetO

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pVE13300

Plasmid

#137715

Purpose

Expresses impLbCas12a in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

Insert

impLbCas12a

Tags

3xHA, NLS, and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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xCas9(3.7)-BE4

Plasmid

#108381

Purpose

Mammalian expression vector for xCas9(3.7)-BE4

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.7)-BE4

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_empty

Plasmid

#203811

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCR1446

Plasmid

#111129

Purpose

Expresses FANCF

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCF (FANCF Human)

Use

Lentiviral

Tags

HA

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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xCas9(3.7)-BE3

Plasmid

#108380

Purpose

Mammalian expression vector for xCas9(3.7)-BE3

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.7)-BE3

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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cacnb2a (rat) in pMT2 vector

Plasmid

#107424

Purpose

Expresses Calcium channel beta2a auxillary subunit in mammalian cells

Depositor

Annette Dolphin

Article

Wyatt et al J Physiol. 1998 Jul 15;510 ( Pt 2):347-6

Insert

calcium channel beta2a auxillary subunit (Cacnb2 Rat)

Expression

Mammalian

Promoter

Ad MLP

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPERretro-Sirt7 shRNA2

Plasmid

#53145

Purpose

shRNAi vector for Sirt7

Depositor

Katrin Chua

Article

Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

Insert

Sirt7

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
pSUPERretro-Sirt7 shRNA1

Plasmid

#53144

Purpose

shRNAi vector for Sirt7

Depositor

Katrin Chua

Article

Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

Insert

Sirt7

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
pYPQ220-Ultra

Plasmid

#176889

Purpose

Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

Insert

AsCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Plant

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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