We narrowed to 7,393 results for: Trac

1. Fast-GCaMP-EF20

   Plasmid

   #52645

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

   Insert

   Fast-GCaMP-EF20
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D362N/D366N mutated from GCaMP3

   Promoter

   T7

   Available Since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pEGFP Tensin2

   Plasmid

   #105298

   Purpose

   To study localization and function of Tensin2

   Depositor

   Kenneth Yamada, David Critchley

   Article

   Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

   Insert

   Tensin2 (TNS2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   lacks N-terminal C1 domain

   Promoter

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB

   Plasmid

   #171427

   Purpose

   Expression of HaloTag N58-(GGS)X6-FRB in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. UAS:Zebrabow-V

   Plasmid

   #118218

   Purpose

   For broad or variegated gal4 inducible expression of Brainbow in zebrafish

   Depositor

   Albert Pan, Alex Schier

   Article

   Pan et al Development. 2013 Jul;140(13):2835-46. d

   Insert

   Brainbow 1.0 L
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. FKBP-AP180-GFP

   Plasmid

   #186577

   Purpose

   Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.

   Depositor

   Stephen Royle

   Article

   Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

   Insert

   AP180 (Snap91 Rat)
   

   Use

   Tags

   FKBP N-terminal GFP C-terminal

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-V96 ELP

   Plasmid

   #67853

   Purpose

   GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

   Depositor

   Andrew MacKay

   Article

   Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

   Insert

   GFP fused to ELP (VPGVG) repeated 96 times
   

   Use

   Tags

   GFP fused to N-terminal of ELP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAWP78-PVCpnf_pvc13-Ad5RGDPK7_Δpvc10

   Plasmid

   #198449

   Purpose

   Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions); also contains truncated spike tip protein (Pvc10).

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-Ad5RGDPK7_Δpvc10
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pN-PITCh-FAID

   Plasmid

   #127885

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   GFP-Puro-H3F-AID (PPP2CA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21b-LaG9(S151C)

   Plasmid

   #172490

   Purpose

   Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   LaG9(S151C)
   

   Use

   Tags

   6xHis, PPX cleavage site, and pelB leader sequenc…

   Expression

   Bacterial

   Mutation

   S151C

   Promoter

   T7

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)

    Plasmid

    #92401

    Purpose

    Constitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

    Depositor

    Periklis Pantazis

    Article

    Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

    Inserts

    Dendra2
    Cerulean
    

    Use

    Zebrafish expression

    Tags

    H2B tag and membrane tag

    Expression

    Mutation

    Promoter

    bactin2

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid

    #74709

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pDEINO3

    Plasmid

    #179487

    Purpose

    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

    Depositor

    Bogumil Karas

    Article

    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

    Insert

    TetR/A
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. untargeted Plk1 FRET sensor Myt1 substrate

    Plasmid

    #45217

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    untargeted Plk1 FRET sensor Myt1 substrate
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    t7

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. Fast-GCaMP-EF16

    Plasmid

    #52642

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D431N/D433N/D435N/E442A mutated from GCaMP3

    Promoter

    T7

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pPBpuro-COX2-EBFP2

    Plasmid

    #184720

    Purpose

    Exogenous COX2

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    COX2-EBFP2 (PTGS2 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CENP-B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45214

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    CENP-B targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    t7

    Available Since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. Fast-GCaMP-EF19

    Plasmid

    #52644

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF19
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T328D/T330D mutated from GCaMP3

    Promoter

    T7

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

    Plasmid

    #31477

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 T2A mTagBFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    F13Y + S111Y on I-AniI = Y2 "reo" (res…

    Promoter

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTS2365-Tier1-PhCMV-TetR

    Plasmid

    #169566

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven tetracycline repressor protein class B from transposon Tn10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid

    #212120

    Purpose

    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    

    Use

    Tags

    His6 and SUMO-TAG35

    Expression

    Bacterial

    Mutation

    E35TAG (within SUMO encoding sequence)

    Promoter

    araC and lpp

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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